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Increased N-Acetyltaurine in the Skeletal Muscle After Endurance Exercise in Rat

  • Conference paper
Taurine 10

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 975))

Abstract

Taurine is metabolized to a novel metabolite, N-acetyltaurine (NAT), through N-acetylation with acetate. Furthermore, NAT production increases when the endogenous production of acetate is elevated in some situations, such as alcohol catabolism and endurance exercise. We have previously reported that both the serum concentration and urinary excretion of NAT from humans were increased after endurance exercise, and that NAT was secreted by cultured skeletal muscle cells exposed to both acetate and taurine. The present study evaluated the hypothesis that NAT is synthesized in the skeletal muscle after endurance exercise. Normal rats were loaded to a transient treadmill running until exhaustion. Serum, skeletal muscle, and liver were collected immediately after the exercise. The NAT concentration in the plasma and in the soleus muscle from the exercised rats was significantly increased compared to that in the samples from the sedentary control rats. There was a significant positive correlation in the NAT concentration between the plasma and soleus muscle. The NAT concentration in the liver was unchanged after the endurance exercise. These results confirm that the significantly increased NAT in both the serum and urine after endurance exercise is derived from NAT synthesis in the skeletal muscle.

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Abbreviations

ACS2:

Acetyl-CoA synthetase 2

EX group:

Exercise group

IS:

Internal standard

NAT:

N-acetyltaurine

PDH:

Pyruvate dehydrogenase

SED group:

Sedentary group

TCA:

Trichloroacetic acid

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Acknowledgments

This study was supported in part by Kakenhi grants (25750334 (Miyazaki 2013–2015)) from the Japan Society for the Promotion of Science.

Author information

Authors and Affiliations

  1. Tokyo Medical University Ibaraki Medical Center, Ibaraki, Japan

    Teruo Miyazaki, Yasushi Matsuzaki & Akira Honda

  2. University of Tsukuba, Ibaraki, Japan

    Yuho Nakamura, Takaki Mizushima & Hajime Ohmori

  3. Seitoku University, Chiba, Japan

    Kei Ebina

  4. Fukuoka University, Fukuoka, Japan

    Song-Gyu Ra

  5. Sojo University, Kumamoto, Japan

    Keisuke Ishikura

Authors
  1. Teruo Miyazaki
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  2. Yuho Nakamura
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  3. Kei Ebina
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  4. Takaki Mizushima
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  5. Song-Gyu Ra
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  6. Keisuke Ishikura
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  7. Yasushi Matsuzaki
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  8. Hajime Ohmori
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  9. Akira Honda
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Corresponding author

Correspondence to Teruo Miyazaki .

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Editors and Affiliations

  1. Department of Life Sciences, University of Seoul, Seoul, South Korea

    Dong-Hee Lee  & Ha Won Kim  & 

  2. College of Medicine, University of South Alabama, Mobile, Alabama, USA

    Stephen W. Schaffer

  3. Department of Developmental Neurobiology, NY State Institute for Basic Research in Developmental Disabilities, New York, USA

    Eunkyue Park

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Miyazaki, T. et al. (2017). Increased N-Acetyltaurine in the Skeletal Muscle After Endurance Exercise in Rat. In: Lee, DH., Schaffer, S.W., Park, E., Kim, H.W. (eds) Taurine 10. Advances in Experimental Medicine and Biology, vol 975. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1079-2_33

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