Summary
A possible interaction of salmon-calcitonin with opioid systems was studied in isolated tissues. Neurogenic contractions were elicited by electrical stimulation in guinea-pig ileum myenteric plexus-longitudinal muscle strips, rabbit vas deferens and mouse vas deferens.
Bremazocine inhibited neurogenic contractions in all three tissues (presumably through κ-receptors) [D-Pen2, D-Pen5]enkephalin and [Met5]enkephalin inhibited contractions in mouse vas deferens (presumably through δ-receptors), and [D-Ala2, N-Me-Phe4, Gly5-ol]enkephalin (DAMGO) inhibited contractions in guinea-pig ileum and mouse vas deferens (presumably through μ-receptors). All inhibitory effects were concentration-dependent. Salmon-calcitonin 0.1 IU/ml increased the effect of bremazocine in guinea-pig ileum and rabbit vas deferens and also increased the effects of [D-Pen2, D-Pen5]enkephalin and [Met5]enkephalin in mouse vas deferens. In contrast, salmon-calcitonin up to 0.4 IU/ml did not change the effect of bremazocine in mouse vas deferens and the effect of DAMGO in guinea-pig ileum and mouse vas deferens.
It is concluded that salmon-calcitonin enhances agonist effects at opioid κ- and δ- but not at opioid μ-receptors. The level of this interaction remains to be elucidated. The interaction may be the basis of the analgesic effect of salmon-calcitonin in vivo.
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Martín, M.I., Alfaro, M.J., Goicoechea, C. et al. In vitro study of the interaction of salmon calcitonin with μ, δ and κ opioid agonists. Naunyn-Schmiedeberg's Arch Pharmacol 347, 324–328 (1993). https://doi.org/10.1007/BF00167452
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DOI: https://doi.org/10.1007/BF00167452