Abstract
The possibility of the involvement of cytokines in the genetic predisposition to various diseases has been suggested by a large variety of studies. However, the study of potential disease linkage of cytokine genes has been hampered by a lack of sufficiently polymorphic markers at the restriction fragment length polymorphism (RFLP) level. We have investigated the distribution, the length polymorphism, the informativeness, and the efficiency of analysis, of simple-sequence tandem repeats in the mouse cytokine genes. Highly polymorphic sequences have been identified in the IL-1β, IL-1ra, IL-2, IL-4, IL-6, IL-7, and IFN-γ genes. The utility and the value of these sequences as gene markers is exemplified by mapping the IL-7 gene to mouse chromosome 3 close to pgk-1ps3 and Car-2 loci and the IFN-γ gene to chrrmosome 10 near the pg locus. Advantages of short tandemly repeated sequences as genetic markers are discussed in comparison with RFLPs.
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Jacob, C.O., Mykytyn, K. & Tashman, N. DNA polymorphism in cytokine genes based on length variation in simple-sequence tandem repeats. Immunogenetics 38, 251–257 (1993). https://doi.org/10.1007/BF00188801
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DOI: https://doi.org/10.1007/BF00188801