Abstract
The Duffy blood group antigens are carried by the erythrocyte membrane glycoprotein gpD, which has a molecular weight of 35–45 kDa and which has been recently cloned. In this report, we have determined, at the nucleic acid level, the molecular basis for the blood group Fya/Fyb polymorphism. The gpD cDNAs isolated by reverse transcription/polymerase chain reaction (RT-PCR) from Fy(a+b−) and Fy(a−b+) donors differed by only one base susbstitution (G131A) changing Gly to Asp at position 44 of the gpD protein. When expressed in simian Cos-7 cells, the Fy(a+b−) and Fy(a-b+) gpD cDNA produce cell surface proteins that react with the anti-Fya and anti-Fyb antisera, respectively, demonstrating that they represent the FY *A and FY *B alleles of the Duffy blood group locus. The G131A nucleotide substitution has been correlated with a BanI restriction site polymorphism, which has allowed us to develop a method for the DNA typing of the main Duffy blood group antigens, by means of PCR/ restriction fragment length polymorphisms.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barnwall JH, Nicholls ME, Rubinstein P (1989) In vitro evaluation of the role of the Duffy blood group in erythrocyte invasion by Plasmodium vivax. J Exp Med 169:1795–1799
Bennett PR, Le Van Kim C, Colin Y, Warwick R, Cherif-Zahar B, Fisk NM, Cartron JP (1993) Prenatal determination of fetal RhD type by DNA amplification. N Engl J Med 329:607–610
Chaudhuri A, Polyakova J, Zbrzezna V, Williams K, Gulati S, Pogo AO (1993) Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite. Proc Natl Acad Sci USA 90: 10793–10797
Chaudhuri A, Zbrzezna V, Polyakova J, Pogo AO, Hesselgessr J, Horuk R (1994) Expression of the Duffy antigen in K562 cells. J Biol Chem 269: 10793–10797
Donahue RP, Bias WB, Renwick JH, McKusick VA (1968) Probable assignment of the Duffy blood group locus to chromosome 1 in man. Proc Natl Acad Sci USA 61:949–955
Le Van Kim C, Mouro I, Brossard Y, Chavinie J, Cartron JP, Colin Y (1994) PCR-based determination of Rhc and RHE status of fetuses at risk of Rhc and RhE hemolytic disease. Br J Haematol 88: 193–195
Lozano EM, Grace O, Romanowski V (1993) Isolation of RNA from whole blood for reliable use in RT-PCR amplification. Trends Genet 9:296
Miller LH, Mason SJ, Clyde DF, McGinnis MH (1976) The resistance factor to Plasmodium vivax in blacks. The Duffy blood group phenotype, FyFy. N Engl J Med 295:302–305
Mollison PL, Engelfriet CP, Contreras M (1993) Blood transfusion in clinical medicine, 9th edn. Blackwell, Oxford
Mouro I, Colin Y, Cherif-Zahar B, Cartron JP, Le Van Kim C (1993) Molecular genetic basis of the human RH blood group system. Nature Genet 5:62–65
Neote K, Mak JY, Kolakowski LF, Schall TJ (1994) Functional and biochemical analysis of the cloned Duffy antigen: identity with the red blood cell chemokine receptor. Blood 84:44–52
Nichols ME, Rubinstein P, Barnwell J, Rodriguez de Cordoba S, Rosenfield R (1987) A new human blood group specificity define by a murine monoclonal antibody. Immunogenetics and association with susceptibility to Plasmodium vivax. J Exp Med 166:776–785
Riwom S, Janvier D, Navenot JM, Benbunan M, Muller JY, Blachard D (1994) Production of a new murine monoclonal antibody with Fy6 specificity and characterization of the immunopurified N-alycosylated Duffy-active molecule. Vox Sang 66:61–67
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tournamille, C., Le Van Kim, C., Gane, P. et al. Molecular basis and PCR-DNA typing of the Fya/fyb blood group polymorphism. Hum Genet 95, 407–410 (1995). https://doi.org/10.1007/BF00208965
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00208965