Summary
Endogenous immunoglobulin-G was localised in ultrathin frozen sections of human term placenta by use of an indirect immuno electron-histochemical methodology. Immunoreactivity of endogenous IgG to rabbit anti-human immunoglobulin-G antibody was visualised by use of protein-A — colloidal gold complex. Gold marked the syncytiotrophoblast in both coated and uncoated regions of the apical plasmalemma, in vesicles and multivesicular bodies, and in vesicles near the basal plasmalemma. Immunoreactivity was also seen in the interstitial space between the trophoblast and the fetal endothelial layer as well as in various types of vesicles within the endothelial cells. No immunoreactivity was seen in the intercellular clefts of the endothelium. The pattern of localisation observed is consistent with receptor-mediated uptake of immunoglobulin-G into the syncytiotrophoblast of the human placenta followed by release into the interstitial space and then vesciular transport through the endothelium.
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References
Ashley CA (1965) Study of the human placenta with the electron microscope. Functional implications of a canal system in the trophoblast. Arch Pathol 80:377–390
Bundgaard M, Frokjaer-Jensen J, Crone C (1979) Endothelial plasmalemma vesicles as elements in a system of branching invaginations from the cell surface. Proc Natl Acad Sci USA 76:5439–6442
Clough G (1982) The steady-state transport of cationized ferritin by endothelial cell vesicles. J Physiol (Lond) 328:389–401
Contractor SF, Eaton BM, Stannard PJ (1983) Uptake and fate of exogenous immunoglobulin G in the perfused human placenta. J Reprod Immunol 5:265–273
Dunn WA, Connolly TP, Hubbard AL (1986) Receptor-mediated endocytosis of epidermal growth factor by rat hepatocytes: Receptor pathway. J Cell Biol 102:24–36
Geuze HJ, Slot JW, Ley PA van der, Scheffer RCT (1981) Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections. J Cell Biol 89:653–665
Geuze HJ, Slot JW, Strous GJAM (1983) Intracellular site of asialoglycoprotein receptor-ligand uncoupling: double-label immunoelectron microscopy during receptor-mediated endocytosis. Cell 32:277–287
Geuze HJ, Slot JW, Strous GJAM, Peppard J, von Figura K, Hasilik A, Schwartz AL (1984) Intracellular receptor sorting during endocytosis: Comparative immunoelectron microscopy of multiple receptors in rat liver. Cell 37:195–204
Gitlin D, Kumate J, Urrusti J, Morales C (1964) The selectivity of the human placenta in the transfer of plasma proteins from mother to fetus. J Clin Invest 43:1938–1951
Goldenthal KL, Hedman K, Chen JW, August JT, Vihko P, Pastan I, Willingham MC (1988) Pre-lysosomal divergence of Alpha-2-macroglobulin and Transferrin: a kinetic study using a monoclonal antibody against a lysosomal membrane glycoprotein (LAMP-1). J Histochem Cytochem 36:391–400
Griffiths GD, Kershaw D, Booth AG (1985) Rabbit peroxidaseantiperoxidase complex (PAP) as a model for the uptake of immunoglobulin G by the human placenta. Histochem J 17:867–881
Hopkins CR (1983) The importance of the endosome in intracellular traffic. Nature 304:684–685
Hopkins CR, Miller K, Beardmore JM (1985) Receptor-mediated endocytosis of transferrin and epidermal growth factor receptors. A comparison of constitutive and ligand-induced uptake. J Cell Sci [Suppl] 3:173–186
Johnson PM, Brown PJ (1981) Fcψ receptors in the human placenta. Placenta 2:355–370
King BF (1982) Absorption of peroxidase-conjugated immunoglobulin G by human placenta: an in vitro study. Placenta 3:395–406
Kuhn LC, Kraehenbuhl JP (1982) The sacrificial receptor — translocation of polymeric IgA across epithelia. Trends Biochem Sci 7:299–302
Lin CT (1980) Immunoelectron microscopic localization of immunoglobulin G in human placenta. J Histochem Cytochem 28:339–346
Pearse BMF (1982) Coated vesicles from human placenta carry ferritin, transferrin and immunoglobulin G. Proc Natl Acad Sci USA 79:451–455
Renkin EM (1985) Capillary transport of macromolecules: pores and other endothelial pathways. J Appl Physiol 58:315–325
Simionescu N, Simionescu M, Palade GE (1973) Permeability of muscle capillaries to exogenous myoglobin. J Cell Biol 57:424–452
Slot JW, Geuze HJ (1985) A new method of preparing gold probes for multiple-labeling cytochemistry. Eur J Cell Biol 38:87–93
Strous GJAM, Willemsen R, van Kerkhof P, Slot JW, Geuze HJ, Lodish HF (1983) Vesicular stomatitis virus glycoprotein, albumin and transferrin are transported to the cell surface via the same golgi vesicles. J Cell Biol 97:1815–1822
Tokuyasu KT (1978) A study of positive staining of ultrathin frozen sections. J Ultrastruct Res 63:287–307
Tokuyasu KT, Singer SJ (1976) Improved procedures for immunoferritin labelling of ultrathin frozen sections. J Cell Biol 71:894–906
Wileman T, Harding C, Stahl P (1985) Review Article: Receptormediated endocytosis. Biochem J 232:1–14
Wood GW, Bjerrum K, Johnson B (1982) Detection of IgG bound within human trophoblast. J Immunol 129:1479–1484
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Leach, L., Eaton, B.M., Firth, J.A. et al. Immunogold localisation of endogenous immunoglobulin-G in ultrathin frozen sections of the human placenta. Cell Tissue Res. 257, 603–607 (1989). https://doi.org/10.1007/BF00221471
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DOI: https://doi.org/10.1007/BF00221471