Summary
The morphological and phenotypical features of multicellular complexes formed by follicular dendritic cells and lymphocytes (FDC-LC) isolated from human hyperplastic tonsils and adenoids are described. FDC-LC obtained with this procedure were morphologically and immuno-phenotypically heterogeneous. In one type of FDCLC, probably obtained from germinal centers, the lymphocytes exhibited ultrastructural features of centroblasts and centrocytes. In a second type, likely derived from follicular mantles, the enclosed lymphocytes were small in size and characterized by a condensed chromatin pattern. Similar heterogeneity was observed by immuno-phenotypical analysis, which revealed a prevalence of IgD+, CD3-, MT2+ small lymphocytes in a high proportion of FDC-LC. Both types of FDC-LC contained desmoplakin immunoreactivity in a typical punctate pattern corresponding to intercellular junctions when tested with a specific antibody. These findings confirm the importance of FDC in maintaining the follicular structure and also suggest that the different zones forming lymphoid follicles (mantle zone and germinal center) are formed by lymphocytes gathered in single “domains” by cytoplasmic processes of FDC. These domains have strong resistance to mechanical stress, such as that used in isolation procedures. FDC-LC have also been maintained as organized multicellular clusters for short periods (more than 48 h) in agarose gel cultures.
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Carbone A, Manconi R, Poletti A, Volpe R, Santi L (1985) S-100 protein immunostaining in cells of dendritic morphology within reactive germinal centers by ABC immunoperoxidase method. Virchows Arch (Pathol Anat) 406:27–32
Carbone A, Poletti A, Manconi R, Gloghini A, Volpe R (1987) Heterogeneous in situ immunophenotyping of follicular dendritic cells in malignant lymphomas of B-cell origin. Cancer 60:2919–2926
Chilosi M, Pizzolo G, Fiore-Donati L, Bofill M, Janossy G (1983) Routine immunofluorescent and histochemical analysis of bone marrow involvement of lymphoma/leukemia. The use of cryostat sections. Br J Cancer 48:763–775
Chilosi M, Pizzolo G, Caligaris-Cappio F, Ambrosetti A, Morittu L, Bonetti F, Fiore-Donati L, Janossy G (1985) Immunohistochemical demonstration of follicular dendritic cells in bone marrow involvement of B-chronic lymphocytic leukemia. Cancer 56:328–332
Chilosi M, Lestani M, Pizzolo G, Fiore-Donati L (1985b) Concerning monocytoid cells. Am J Clin Pathol 884:573–574
Chilosi M, Menestrina F, Lestani M, Scarpa A, Bonetti F, Pizzolo G, Fiore-Donati L (1986) Growth fraction analysis of heterogeneous cell populations using a combined enzyme-histochemical and immunohistochemical technique with Ki67 monoclonal antibody [Abstr]. Basic Appl Histochem 30:55
Chilosi M, Menestrina F, Lestani M, Bonetti F, Scarpa A, Caligaris-Cappio F, Pizzolo G, Perini A, Fiore-Donati L (1987) Hyaline-vascular type of Castleman disease (angiofollicular lymph node hyperplasia) with monotypic plasma cells. An immunohistochemical study with monoclonal antibodies. Histol Histopathol 2:49–55
Coico RF, Bhogal BS, Thorbecke GJ (1983) Relationship of germinal center in lymphoid tissue to immunologic memory. VI.Transfer of B cell memory with lymph node cells fractionated according to their receptors for peanut agglutinin. J Immunol 131:2254–2257
Cormann N, Lesage F, Heinen E, Schaaf-Lafontaine N, Kinet-Denoel C, Simar LJ (1986) Isolation of follicular dendritic cells from human tonsils and adenoids. V. Effect on lymphocyte proliferation and differentiation. Immunol Lett 14:29–35
Gerdes J, Stein H, Mason DY, Ziegler A (1983) Human dendritic reticulum cells of lymphoid follicles: their antigenic profile and their identification as multinucleated giant cells. Virchows Arch (Cell Pathol) 42:161–172
Heinen E, Lilet-Leclercq C, Mason DY, Stein H, Boniver J, Radoux D, Kinet-Denoel C, Simar LJ (1984) Isolation of follicular dendritic cells from human tonsils and adenoids. II. Immunocytochemical characterization. Eur J Immunol 14:267–273
Janossy G, Pinching AJ, Bofill M, Weber J, McLaughlin JE, Ornstein M, Ivory K, Harris JRW, Favrot M, Mac-donald BurnsDC (1985) An immunohistological approach to persistent lymphadenopathy and its relevance to acquired immune deficiency syndrome. Clin Exp Immunol 59:257–266
Kojima M, Imai Y (1973) Genesis and function of germinal center. GANN 15:1–24
Kraal G, Weissman IL, Butcher EC (1982) Germinal center B cells: antigen specificity and changes in heavy chain class expression. Nature 298:377–379
Lea T, Vartdal F, Davies C, Ugelstad J (1985) Magnetic monosized polymer particle for fast and specific fractionation of human mononuclear cells. Scand J Immunol 22:207–216
Lilet-Leclercq C, Radoux D, Heinen E, Kinet-Denoel C, Defraigne JO, Houben-Defresne MP, Simar LJ (1984) Isolation of follicular dendritic cells from human tonsils and adenoids. I. Procedure and morphological characterization. J Immunol Methods 66:235–244
Manconi PE, Ennas MG, Murru MR, Cadeddu G, Tore G, Lantini MS (1984) Epithelial-like cells containing lymphocytes (nurse cells) in human adenoids and tonsils. Thymus 6:351–357
Mandel TE, Phipps RP, Abbot A, Tew JG (1980) The follicular dendritic cell: long term antigen retention during immunity. Immunol Rev 53:29–59
Nossal GJV, Abbott A, Mitchell J, Lummus Z (1968) Antigens in immunity XV. Ultrastructural features of antigen capture in primary and secondary lymphoid follicles. J Exp Med 127:277–290
Pizzolo G, Chilosi M (1984) Double immunostaining of tissue sections by monoclonal antibodies using phycoerythrin labelling and haptenated reagents. Am J Clin Pathol 82:44–47
Poppema S, Hollema H, Visser L, Vos H (1987) Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections. Am J Pathol 127:418–429
Stein H, Gerdes J, Mason DY (1982) The normal and malignant germinal centre. Clin Haematol 11:531–559
Tew JG, Phipps RP, Mandel TE (1980) The maintenance and regulation of the humoral immune response: persisting antigen and the role of follicular antigen-binding dendritic cells as accessory cells. Immunol Rev 53:175–201
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Supported in part by: Ministero Pubblica Istruzione, Italy, and A.I.R.C., Milano, Italy
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Ennas, M.G., Chilosi, M., Scarpa, A. et al. Isolation of multicellular complexes of follicular dendritic cells and lymphocytes: Immunophenotypical characterization, electron microscopy and culture studies. Cell Tissue Res. 257, 9–15 (1989). https://doi.org/10.1007/BF00221628
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DOI: https://doi.org/10.1007/BF00221628