Abstract
We have used the polymerase chain reaction to amplify cDNA from expressed bovine major histocompatibility complex class I genes. Sequences obtained from transmembrane and cytoplasmic domains were used to identify the number of expressed alleles. Data from three animals suggest that there are four major expressed alleles, representing the products of two (or more) loci. We have also demonstrated the presence of an alternatively spliced mRNA, which has been observed in five animals. The alternative splicing removes exon 7 (the major site of class I phosphorylation), which predicts a truncated molecule with a cytoplasmic portion 16 amino acids shorter than usual. This phenomenon was detected for only a single class I allele within each individual.
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Badley, J. E., Bishop, G. A., St. John, T., and Frelinger, J. A.: A simple, rapid method for the purification of Poly A + RNA. Biotechniques 6: 114–116, 1988
Bensaid, A., Naessons, J., Kemp, S. J., Black, S. J., Shapiro, S. Z., and Teale, A. J.: An immunochemical analysis of class I (BoLa) molecules on the surface of bovine cells. Immunogenetics 27: 139–144, 1988
Bensaid, A., Kaushal, A., MacHugh, N. D., Shapiro, S. Z., and Teale, A. J.: Biochemical characterization of activation-associated bovine class I MHC antigens. Anim Genet 20: 241–255, 1989
Bensaid, A., Kaushal, A., Baldwin, C. L., Clevers, H., Young, J. R., Kemp, S. J., MacHugh, N. D., Toye, P. G., and Teale, A. J.: Identification of expressed bovine class I MHC genes at two loci and demonstration of physical linkage. Immunogenetics 33: 247–254, 1991
Brown, P., Spooner, R. L., and Clark, A. J.: Cloning and characterization of a BoLa class I cDNA clone. Immunogenetics 29: 58–60, 1989
Bull, R. W.: Joint Report of the Third International bovine Antigen (BoLa) Workshop, Helsinki, 1986. Anim Genet 20: 109–132, 1989
Dominski, Z. and Kole, R.: Selection of splice sites in pre-mRNAs with short internal exons. Mol Cell Biol 11: 6075–6083, 1991
Ellis, S. A., Palmer, M. S., and McMichael, A. J.: Human trophoblast and the choriocarcinoma cell line BeWo express a truncated HLA class I molecule. J Immunol 144: 731–735, 1990
Ennis, P. D., Jackson, A. P., and Parham, P.: Molecular cloning of bovine class I MHC cDNA. J Immunol 141: 642–651, 1988
Ennis, P. D., Zemmour, J., Salter, R. D., and Parham, P.: Rapid cloning of HLA-A, B cDNA by using the polymerase chain reaction: frequency an nature of errors produced in amplification. Proc Natl Acad Sci USA 87: 2833–2837, 1990
Epstein, H.: Origin of the domestic animals of Africa. Africana Publishing Corporation, NY, 1971
Glass, E. J. and Spooner, R. L.: Parasite-accessory cell interactions in Theileriosis. Eur J Immunol 20: 2491–2497, 1990
Grossberger, D., Hein, W., and Marcuz, A.: Class IMHC cDNA clones from sheep thymus: alternative splicing could make a longer cytoplasmic tail. Immunogenetics 32: 77–87, 1990
Guild, B. C. and Strominger, J. L.: Human and murine class I MHC antigens share conserved serrine 335, the site of HLA phosphorylation in vivo. J Biol Chem 259: 9235–9240, 1984
Gussow, D., Rein, R. S., Meijer, I., de Hoog, W., Seemann, G. H. A., Hochstenbach, F. M., and Ploegh, H. L.: Isolation, expression and the primary structure of HLA-Cwl and HLA-Cw2 genes: evolutionary aspects. Immunogenetics 25: 313–322, 1987
Kemp, S. J., Spooner, R. L., and Teale, A. J.: A comparative study of MHC antigens in East African and European cattle breeds. Anim Genet 19: 17–29, 1988
Kemp, S. J., Tucker, E. M., and Teale, A. J.: A bovine monoclonal antibody detecting a class I BoLA antigen. Anim Genet 21: 153–160, 1990
Krangel, M. S.: Secretion of HLA-A and-B antigens via an alternative RNA splicing pathway. J Exp Med 163: 1173–1190, 1986
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685, 1970
Lalanne, J.-L., Transy, C., Guerin, S., Darche, S., Meuhen, P., and Kourilsky, P.: Expression of class I genes in the mouse MHC: identification of eight distinct mRNAs in DBA/2 mouse liver. Cell 41: 469–478, 1985
Lawlor, D. A., Dichel, C. D., Hauswirth, W. W., and Parham, P.: Ancient HLA genes from 7500 year old archeological remains. Nature 349: 785–787, 1991
Lew, A. M., Margulies, D. H., Maloy, D. H., Lillehoj, W. L., McCluskey, J., and Coligan, J. E.: Alternative protein products with carboxyl termini from a single class I gene, H-2K b. Proc Natl Acad Sci USA 83: 6084–6088, 1986
Lew, A. M., McCluskey, J., Maloy, W. L., Margulies, D. H., and Coligan, J. E.: Multiple class I molecules generated from single genes by alternative splicing of pre-mRNAs. Immunol Res 6: 117–132, 1987
Maniatis, T., Fritsch, E. F., and Sambrook, J.: Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, 1982
Matzinger, P., Zamoyska, R., and Waldmann, H.: Self tolerance is H-2 restricted. Nature 308: 738–741, 1984
Mizuno, S., Ng, J., Dupont, B., and Yang, S. Y.: The cloning, exon shuffling, and expression of the HLA Cw1 gene. In B. Dupont (ed.): Immunobiology of HLA, vol 2. pp. 138–140, Springer-Verlag, New York, 1989
Parham, P., Lomen, C. E., Lawlor, D. A., Way, J. P., Holmes, N., Coppen, H. L., Salter, R. D., Wan, A. M., and Ennis, P. D.: Nature of polymorphism in HLA-A,-B and-C molecules. Proc Natl Acad Sci USA 85: 4005–4009, 1988
Robinson, M. A. and Kindt, T. J.: Major histocompatibility complex antigens and genes. In W. E. Paul (ed.): Fundamental Immunology. pp. 489–539, Raven Press, New York, 1989
Sanger, F., Nicklen, S., and Coulson, A. R.: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467, 1977
Spooner, R. L. and Brown, C. G. D.: Bovine lymphocyte antigens (BoLa) of bovine lymphocytes and derived lymphoblastoid lines transformed by Theileria parva and Theileria anulata. Parasite Immunol 2: 163–174, 1980
Stroynowski, I.: Molecules related to class I MHC antigens. Annu Rev Immunol 8: 501–530, 1990
Toye, P. G., MacHugh, N. D., Bensaid, A. M., Alberti, S., Teale A. J., and Morrison, W. I.: Transfection into mouse L cells of genes encoding 2 serologically and functionally distinct bovine class I MHC molecules from a MHC-homozygous animal: evidence for a second class I locus in cattle. Immunology 70: 20–26, 1990
Transy, C., Lalanne, J.-L., and Kourilsky, P.: Alternative splicing in the 5’ moiety of the H-2Kd gene transcript. EMBO J 3: 2383–2386, 1984
Ulker, N., Lewis, K. D., Hood, L. E., and Stroynowski, I.: Activated T cells transcribe an alternatively spliced mRNA encoding a soluble form of Qa-2 antigen. EMBO J 9: 3839–3847, 1990
Vogel, J. M., Morse, R. Y., and Goodenow, R. S.: A novel H-2K splice form: predictions for other alternative H-2 splicing events. Immunogenetics 29: 33–43, 1989
Yewdell, J., Bennink, J., and Hosaka, Y.: Cells process exogenous proteins for recognition bycytotoxic T lymphocytes. Science 239: 637–640, 1988
Zuniga, M. C. and Hood, L. E.: Clonal variation in cell surface display of an H-2 protein lacking a cytoplasmic tail. J Cell Biol 102: 1–10, 1986
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Correspondence to: S. A. Ellis.
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Ellis, S.A., Braem, K.A. & Morrison, W.I. Transmembrane and cytoplasmic domain sequences demonstrate at least two expressed bovine MHC class I loci. Immunogenetics 37, 49–56 (1992). https://doi.org/10.1007/BF00223544
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DOI: https://doi.org/10.1007/BF00223544