Summary
Sex reversed mice heterozygous for the X-linked Tfm mutation are mosaics with respect to the Tfm locus. In the androgen-dependent striated urethral muscle, nuclei coding for the intact testosterone receptor protein and nuclei coding for the defective Tfm receptor protein are incorporated in the same multinucleate muscle fibres. The intact testosterone receptor complex can thus be expected to enter the Tfm nuclei. Our measurements show that the fibre diameters of the mosaic muscle form a homogeneous population, intermediate in size between induced male and non-inducible Tfm phenotypes. By contrast, the nuclear size shows a bimodal distribution, the subpopulations corresponding to Tfm and wild type nuclei. The results indicate that the Tfm nuclei are not activated by the intact testosterone receptor complex.
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References
Attardi B, Ohno S (1974) Cytosol androgen receptor from kidney of normal and testicular feminized (Tfm) mice. Cell 2:205–212
Boguth W, Langendorff H, Tonutti E (1951) Zellkerngröße als Indikator der Funktionsbeziehungen Hypophyse-Nebennierenrinde. Med. Welt 20:408–414
Britten RJ, Davidson EH (1969) Gene regulation for higher cells: A theory. Science 165:349–357
Cattanach BM, Pollard CE, Hawkes SG (1971) Sex reversed mice: XX and XO males. Cytogenetics 10:319–337
Daeves K, Beckel A (1958) Großzahl-Methodik und Häufigkeitsanalyse, 2. Aufl., Weinheim, p 143
Drews U (1975) Direct and mediated effects of testosterone: The development of intersexes in sex reversed mosaic mice, heterozygous for testicular feminisation. Anat Embryol 146:325–340
Drews U (1978) Cell interactions in the sex organs of sex reversed mice heterozygous for testicular feminization. In: Russell LB (ed) Genetic mosaics and chimeras in mammals, Plenum Publ Co, pp 183–193
Drews U, Alonso-Lozano V (1974) X-inactivation pattern in the epididymis of sex-reversed mice heterozygous for testicular feminization. J Embryol Exp Morphol 32:217–225
Drews U, Drews U (1975) Metabolic cooperation between Tfm and wild-type cells in mosaic mice after induction of DNA synthesis. Cell 6:475–479
Fetzer S, Hillebrecht J, Muschke HE, Tonutti E (1955) Hypophysäre Steuerung der interstitiellen Zellen des Rattenovariums, quantitativ betrachtet am Zellkernvolumen. Z Zellforsch 43:404–420
Gehring U, Tomkins GM (1974) Characterization of a hormone receptor defect in the androgen-insensitivity mutant. Cell 3:59–64
Lyon MF, Hawkes SG (1970) X-lined gene for testicular feminization in the mouse. Nature (Lond) 227:1217–1219
Mainwaring WJP (1978) Androgen receptors and biologic responses: a survey. In: O'Malley BW, Birnbaumer L (eds) Receptors and hormone action. New York, Vol II, pp 105–120
Muschke HE (1953) Histometrische Untersuchungen am Rattenhoden nach Hypophysektomie und nach Choriongonadotropinzufuhr. Endokrinologie 30:281–294
Ohno S, Lyon MF (1970) X-linked testicular feminization in the mouse as a non-inducible regulatory mutation of the Jacob-Monod type, Clin Genet 1:121–127
Pfanzagl J (1978) Allgemeine Methodenlehre der Statistik, Vol II, Walter de Gruyter, Berlin, pp 41–44
Tukey JW (1977) Exploratory data analysis. Addison-Wesley, London
Venable JH (1966) Constant cell populations in normal, testosterone-deprived and testosterone-stimulated levator ani muscles. Am J Anat 119:263–270
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Thiedemann, K.U., Drews, U. Nuclei in testicular feminization (Tfm) are not activated by intact testosterone receptor complexes: A morphometric study in striated urethral muscle of mosaic mice. Cell Tissue Res. 212, 127–138 (1980). https://doi.org/10.1007/BF00234039
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DOI: https://doi.org/10.1007/BF00234039