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Inhibition of human breast cancer colony formation by anticalmodulin agents: Trifluoperazine, W-7, and W-13

  • Original Articles
  • Breast Cancer Trifluoperazines
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Summary

The effects of anticalmodulin agents, namely trifluoperazine (TFP) and two naphthalene sulfonamide derivatives (W-7 and W-13), were tested on the growth of a human breast cancer cell line (MDA-MB-231) using a soft agar clonogenic assay. The results of this in vitro study reveal that TFP, W-7, and W-13 had the ability to inhibit the colony formation from this cell line. The inhibitory effect was greater when the cancer cells were exposed to these agents continuously than when the cells were exposed to the drugs for 1 h. The IC50 values for TFP, W-7, and W-13 in continuous exposure were about 18, 30, and 38 μM, respectively, whereas the corresponding values for 1-h exposure were 50, 53, and 70 μM, respectively. These findings suggest that anticalmodulin agents can inhibit the growth of human cancer cells at relatively low concentrations in vitro. Whether effective antitumor concentrations of these drugs can be achieved in vivo remains a subject for further study.

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Authors and Affiliations

  1. Department of Pharmacology, University of Saskatchewan, S7N OWO, Saskatoon, Saskatchewan, Canada

    Jiann-Wu Wei & Robert A. Hickie

  2. Division of Oncology, College of Medicine, University of Saskatchewan, S7N OWO, Saskatoon, Saskatchewan, Canada

    David J. Klaassen

Authors
  1. Jiann-Wu Wei
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  2. Robert A. Hickie
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  3. David J. Klaassen
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Wei, JW., Hickie, R.A. & Klaassen, D.J. Inhibition of human breast cancer colony formation by anticalmodulin agents: Trifluoperazine, W-7, and W-13. Cancer Chemother. Pharmacol. 11, 86–90 (1983). https://doi.org/10.1007/BF00254251

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  • DOI: https://doi.org/10.1007/BF00254251

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