Summary
Renal glomerular structures have been studied during the initial phases of renal and glomerular hypertrophy in streptozotocin diabetic rats. — After four days of diabetes significant increases were found in the following absolute structural quantities: the tuft volume (25%), the surface area (42%) and volume (46%) of peripheral basement membrane, and capillary luminal volume (29%). No further changes in these quantities took place over the succeeding 47 days. The geometry of the capillaries, however, changed over this period: at 4 days the total capillary length had increased significantly (32%) with an unchanged average cross sectional area, whereas at 47 days the length had reverted to normal, but average cross sectional area had increased (30%). The increased surface of filtering area may be the morphological counterpart of increased glomerular filtration rate. The changes in glomerular volume were thus accompanied by changes in glomerular structural composition which after a few weeks returned to normal, in balance with the increased volume.
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Seyer-Hansen K, Hansen J, Gundersen HJG (1980) Renal hypertrophy in experimental diabetes: A morphometric study. Diabetologia 18: 501–505
Østerby R, Gundersen HJG (1975) Glomerular size and structure in diabetes mellitus. I. Early abnormalities. Diabetologia 11: 225–229
Kroustrup JP, Gundersen HJG, Østerby R (1977) Glomerular size and structure in diabetes mellitus. III. Early enlargement of the capillary surface. Diabetologia 13: 207–210
Østerby R, Gundersen HJG (1978) Sampling problems in the kidney. In: Miles RE, Serra J (eds) Lecture notes in biomathematics, vol 23. Springer, Berlin Heidelberg New York, p 185–191
Weibel ER (1973) Stereological techniques for electron microscopic morphometry. In: Hayat MA (ed) Principles and techniques of electron microscopy, vol 3. van Nostrand Reinhold, New York, p 237–296
Jensen EB, Gundersen HJG, Østerby R (1979) Determination of membrane thickness distribution from orthogonal intercepts. J Microsc 115: 19–33
Weibel ER, Knight BW (1964) A morphometric study on the thickness of the pulmonary air-blood barrier. J Cell Biol 21: 367–384
Diem K, Lentner C (1970) Scientific tables, 7th ed. Geigy, Basel, p 171
Sargent F, Weinman KP (1966) Physiological individuality. In: Weyer EM (ed) The biology of human variation, Annals of the New York Academy of Sciences, vol 134. New York, p 696–719
Price RG, Spiro RG (1977) Studies on the metabolism of the renal glomerular basement membrane. J Biol Chem 252: 8597–8602
Cohen MP, Klein CV (1979) Glomerulopathy in rats with streptozotocin diabetes. Accumulation of glomerular basement membrane analogous to human diabetic nephropathy. J Exp Med 149: 623–631
Spiro RG, Spiro MJ (1971) Effect of diabetes on the biosynthesis of the renal glomerular basement membrane: Studies on the glycosyltransferase. Diabetes 20: 641–648
Cohen MP, Khalifa A (1977) Effect of diabetes and insulin on rat renal glomerular protocollagen hydroxylase activities. Biochim Biophys Acta 496: 88–94
Seyer-Hansen K (1976) Renal hypertrophy in streptozotocin-diabetic rats. Clin Sci Mol Med 51: 551–555
Rasch R (1979) Prevention of diabetic glomerulopathy in streptozotocin diabetic rats by insulin treatment: Kidney size and glomerular volume. Diabetologia 16: 125–128
Rasch R (1979) Prevention of diabetic glomerulopathy in streptozotocin diabetic rats by insulin treatment. Glomerular basement membrane thickness. Diabetologia 16: 319–324
Rasch R (1979) Prevention of diabetic glomerulopathy in streptozotocin diabetic rats by insulin treatment: The mesangial regions. Diabetologia 17: 243–248
Mogensen CE (1972) Kidney function and glomerular permeability to macromolecules in juvenile diabetes. Dan Med Bull 19 [Suppl 3]: 1–38
Hirose K, Østerby R (1979) A strong structure-function relationship between glomerular filtration rates and filtration surface areas in short-term diabetics (Abstract). In: Waldhäusl W, Alberti KGMM (eds) International Congress Series no 481. Excerpta Medica, Amsterdam, p 94
Mogensen CE (1975) Increased kidney size and glomerular filtration rate in untreated juvenile diabetes: Normalization by insulin-treatment. Diabetologia 11: 221–224
G0tzsche O, Gundersen HJG, Østerby R (1979) Reversibility and non-reversibility of renal morphological changes in early experimental diabetes after pancreatic transplantation (Abstract). Acta Endocrinol [Suppl] (Kbh) 227: 27–28
Shea SM (1979) Glomerular hemodynamics and vascular structure. Microvasc Res 18: 129–143
Steffes MW, Brown DM, Basgen JM, Matas AJ, Mauer SM (1979) Glomerular basement membrane thickness following islet transplantation in the rat. Lab Invest 41: 116–118
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Østerby, R., Gundersen, H.J.G. Fast accumulation of basement membrane material and the rate of morphological changes in acute experimental diabetic glomerular hypertrophy. Diabetologia 18, 493–500 (1980). https://doi.org/10.1007/BF00261706
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DOI: https://doi.org/10.1007/BF00261706