Summary
The effect of High energy shock waves (HESW) on the viability and proliferation of normal human bone marrow cells was evaluated. A dose dependent increase in cytotoxicity with an increase in the number of HESW was demonstrated. In general 700 HESW immediately reduced the cell viability of bone marrow cells by 50%. The CFU-GM assay provides a method to evaluate the effect of HESW on the proliferative capacity of bone marrow. Following HESW treatment the colony forming ability of trypan blue excluding cells also felt in a dose dependent fashion, but some variation in sensitivity was noted. By comparing the sensitivity of various cells, the cells of normal human bone marrow were felt to be less sensitive to HESW effects than those of other tissue cultured cells or malignant cell lines.
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References
Chang TT, Gulati S, Chou TC, Colvin M, Clarkson B (1987) Comparative cytotoxicity of various drug combinations for human leukemic cells and normal hematopoietic precursors. Cancer Res 47:119–122
Pike BL, Robinson WA (1970) Human bone marrow culture in agar-gel. J Cell Physiol 76:77–84
Russo P, Hoston WDW, Fair WR (1985) Suppression of in vitro and in vivo tumor growth by high-energy shock waves. Surg Forum 36:646
Russo P, Stephenson RA, Mies C, Huryk R, Heston WDW, Melamed MR, Fair WR (1986) High energy shock waves suppress tumor growth in vitro and vivo. J Urol 135:626–628
Yang CR, Badalament R, Traganos F, Bueti C, Huryk R, Heston WDW, Fair WR (in press) High energy shock waves — effects on cell kinetics.
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Fellow at Memorial Sloan-Kettering Cancer Center supported in part by Veterans General Hospital-Taichung, Taichung, Taiwan, Republic of China
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Yang, C., Heston, W.D.W., Gulati, S. et al. The effect of High energy shock waves (HESW) on human bone marrow. Urol. Res. 16, 427–429 (1988). https://doi.org/10.1007/BF00280023
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DOI: https://doi.org/10.1007/BF00280023