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Two biochemical indices of mouse bone formation are increased, in vivo, in response to calcitonin

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Summary

In a series of four studies, adult female Swiss-Webster mice were used to measure the effects of salmon calcitonin on two biochemical indices of local and systematic bone formation: (1) skeletal alkaline phosphatase activity—in serum and in extracts of calvaria and tibiae, and (2) calvarial collagenase-digestible protein synthesis—measured, acutely, in vitro. Subcutaneous calcitonin doses ranged from 50 to 400 mU/mouse/day (0.95–18.1 U/kg/day), and treatment schedules were continuous (daily) for 2–14 days, acute, or intermittent (2 days/week for 6 weeks). The effects of calcitonin on these bone formation indices (skeletal alkaline phosphatase and collagenase-digestible protein synthesis) were biphasic with respect to dose and treatment time, being increased in response to short-term, low-dose treatment, but not long-term, continuous treatment. The effects of long-term intermittent calcitonin treatment were dose-dependent increases in skeletal alkaline phosphatase in calvaria and serum (r=0.948, P< 0.02, and r=0.960, P< 0.01, respectively).

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Authors and Affiliations

  1. Department of Medicine and Biochemistry, Loma Linda University, and Research Service (151),the Jerry L. Pettis Memorial Veterans' Hospital, 11201 Benton Street, 92357, Loma Linda, California, USA

    John R. Farley, Susan L. Hall, Sandra Herring & Nanine M. Tarbaux

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  1. John R. Farley
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  2. Susan L. Hall
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  3. Sandra Herring
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  4. Nanine M. Tarbaux
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Farley, J.R., Hall, S.L., Herring, S. et al. Two biochemical indices of mouse bone formation are increased, in vivo, in response to calcitonin. Calcif Tissue Int 50, 67–73 (1992). https://doi.org/10.1007/BF00297300

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  • DOI: https://doi.org/10.1007/BF00297300

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