Summary
The reaction of neural structures of the medial basal hypothalamus (MBH) to its complete deafferentation was studied in male rats by means of enzyme-histochemical and histoautoradiographic methods. Particular attention was paid to nerve cells of the arcuate nucleus and to the tanycytes. The metabolic activity of these cells increased upon the whole.
According to the indices of metabolic activity in normal conditions and following deafferentation, the authors distinguish among the ependymal cells of the recessus infundibularis in rats, α-tanycytes, which correspond to the ependymal lining at the level of the arcuate nucleus, and β-tanycytes, which correspond to the ependyma of the median eminence. In normal conditions both were marked by a sufficiently high level of metabolism, which did not exclude the possibility of protein synthesis. Following deafferentation, α-tanycytes seemed most “reactive”. The most active elements among the β-tanycytes were the ependymal cells of the lateral part of the median eminence (β1-tanycytes).
The metabolic peculiarities of the nerve cells of the arcuate nuclei and the tanycytes, revealed in normal conditions and after deafferentation, are discussed in connection with the modern concepts of the role of these cells in hypothalamic-hypophyseal transmission.
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References
Akmayev, I.G., Réthelyi, M., Majorossy, K.: Changes induced by adrenalectomy in nerve endings of the hypothalamic median eminence (zona palisadica) in the albino rat. Acta biol. Acad. Sci. hung. 18, 187–200 (1967).
Anand Kumar, T.C.: Sexual differences in the ependyma lining the third ventricle in the area of the anterior hypothalamus of adult rhesus monkeys. Z. Zellforsch. 90, 28–36 (1968).
Arko, H., Kivalo, E., Rinne, U.K.: Hypothalamo-neurohypophysial neurosecretion after the extirpation of various endocrine glands. Acta endocr. (Kbh.) 42, 293–299 (1963).
Babichev, V.N.: Interaction of hypothalamic areas taking part in the regulation of gonadotropic function of the hypophysis in female rats. Probl. endocr. 17, 82–85 (1971) [Russ.]
Bargmann, W.: Das neurosekretorische Zwischenhirn-Hypophysensystem und seine synaptischen Verknüpfungen. J. Neuro-Viscer. Relat., Suppl. 9, 64–77 (1969).
Bargmann, W.: Die funktionelle Morphologie des endokrinen Regulationssystems. In: Handbuch der Allgemeinen Pathologie, Bd. VIII, T. I, S. 1–106 (hrsgeg. von H.W. Altmann et al.). Berlin-Heidelberg-New York: Springer 1971.
Bargmann, W., Lindner, E., Andres, K.H.: Über Synapsen an endokrinen Epithelzellen und die Definition sekretorischer Neurone. Untersuchungen am Zwischenlappen der Katzenhypophyse. Z. Zellforsch. 77, 282–298 (1967).
Bargmann, W., Scharrer, E.: The site of origin of the hormones of the posterior pituitary. Amer. Sci. 39, 255–259 (1951).
Björklund, A., Falck, B., Hromek, F., Owman, Ch., West, K.A.: Identification and terminal distribution of the tubero-hypophyseal monoamine fibre systems in the rat by means of stereotaxic and microspectrofluorimetric techniques. Brain Res. 17, 1–23 (1970).
Bock, R., Aus der Mühlen, K.: Beiträge zur funktionellen Morphologie der Neurohypophyse. I. Über eine „gomoripositive“ Substanz in der Zona externa infundibuli beidseitig adrenalektomierter weißer Mäuse. Z. Zellforsch. 92, 130–148 (1968).
Bock, R., Goslar, H.-G.: Enzymhistochemische Untersuchungen an Infundibulum und Hypophysenhinterlappen der normalen und beidseitig adrenalektomierten Ratte. Z. Zellforsch. 95, 415–428 (1969).
Brinkmann, H., Bock, R.: Quantitative Veränderungen „Gomori-positiver“ Substanzen in Infundibulum und Hypophysenhinterlappen der Ratte nach Adrenalektomie und Kochsalz- oder Durstbelastung. J. Neuro-Viscer. Relat. 32, 48–64 (1970).
Christ, J.: Zur Anatomie des Tuber cinereum beim erwachsenen Menschen. Dtsch. Z. Nervenheilk. 165, 340–408 (1951).
Clementi, F., Ceccarelli, B.: Fine structure of rat hypothalamic nuclei. In: The hypothalamus (ed. by L. Martini, M. Motta, F. Fraschini). New York: Academic Press Inc. 1970.
Colmant, H. J.: Über die Wandstruktur des dritten Ventrikels der Albinoratte. Histochemie 11, 40–61 (1967).
Egdahl, R.H.: Cerebral cortical inhibition of pituitary-adrenal secretion. Endocrinology 68, 574–581 (1961).
Feldberg, W., Fleischhauer, K.: Penetration of bromophenol blue from the perfused cerebral ventricles into the brain tissue. J. Physiol. (Lond.) 150, 451–462 (1960).
Feldman, S., Sarne, Y.: Effect of cortisol on single cell activity in hypothalamic islands. Brain Res. 23, 67–75 (1970).
Goebel, F.-D.: Neue Ergebnisse zur Morphologie neurosekretorischer Systeme im Hypothalamus und in der Neurohypophyse der normalen und adrenalektomierten Maus. Diss. Bonn. 1968.
Goslar, H.-G., Bock, R.: Zur Spaltbarkeit verschiedener Naphtol-Carbonsäureester durch Esterasen im Tanycytenependym des III. Ventrikels der Wistarratte. Histochemie 21, 353–365 (1970).
Goslar, H.-G., Bock, R.: Histochemische Eigenschaften der unspezifischen Esterasen im Tanycytenependym des III. Ventrikels, im Subfornicalorgan und im Subcommissuralorgan der Wistarratte. Histochemie 28, 170–182 (1971).
Halász, B.: Neurohormonal mechanisms controlling trophic hormone secretion of the anterior pituitary. J. Neuro-Viscer. Relat., Suppl. 9, 329–361 (1969).
Halász, B., Pupp, L.: Hormone secretion of the anterior pituitary gland after physical interruption of all nervous pathways to the hypophysiotrophic area. Endocrinology 77, 553–562 (1965).
Halász, B., Réthelyi, M., Szentágothai, J.: Examens électromicroscopiques sur l'éminence médiane isolée (désafférentée neuralement). Arch. Anat. (Lausanne) 51, 289–298 (1968).
Halász, B., Slusher, M.A., Gorski, R.A.: Adrenocorticotrophic hormone secretion in rats after partial or total deafferentation of the medial basal hypothalamus. Neuroendocrinology 2, 43–55 (1967).
Hild, W., Takenaka, T., Walker, F.: Electrophysiological properties of ependymal cells from the mammalian brain in tissue culture. Exp. Neurol. 11, 493–501 (1965).
Horstmann, E.: Die Faserglia des Selachiergehirns. Z. Zellforsch. 39, 588–617 (1954).
Ishii, S.: Classification and identification of neurosecretory granules in the median eminence. Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich 1971, p. 119–141. Basel: Karger 1972.
Knowles, Sir F.: Neuronal properties of neurosecretory cells. Neurosecretion. IV. Int. Symp. on Neurosecretion (ed. by F. Stutinsky), p. 8–19. Berlin-Heidelberg-New-York: Springer 1967.
Knowles, Sir F.: Ependymal secretion, especially in the hypothalamic region. J. Neuro-Viscer. Relat., Suppl. 9, 97–110 (1969).
Knowles, Sir F.: Secretory cells in the ependyma. In: Memoirs of the Society for Endocrinology No 19 “Subcellular organization and function in endocrine tissues”, p. 875–878 (ed. by H. Heller and K. Lederis). London-New York: Cambridge Univ. Press 1971.
Knowles, Sir F., Anand Kumar, T.C.: Structural changes, related to reproduction, in the hypothalamus and in the pars tuberalis of the Rhesus monkey. Part I. The hypothalamus. Part II. The pars tuberalis. Phil. Trans. B 256, 357–375 (1969).
Knowles, Sir F., Vollrath, L.: Neurosecretory innervation of the pituitary of the eels, Anguilla and Conger. I. The structure and ultrastructure of the neuro-intermediate lobe under normal and experimental conditions. II. The structure and innervation of the pars distalis at different stages of the life-cycle. Phil. Trans. B 250, 311–342 (1966).
Kobayashi, H., Ishii, S.: The median eminence as storage site for releasing factors and other biologically active substances. Excerpta Med. Int. Congr. Ser. No 184, Progress in Endocrinology, p. 548–554 (1968/1969).
Kobayashi, H., Matsui, T.: Fine structure of the median eminence and its functional significance. In: Frontiers in Neuroendocrinology, p. 3–46 (ed. by W.F. Ganong and L. Martini). Oxford: Univ. Press, Inc. 1969.
Leonhardt. H.: Über ependymale Tanycyten des III. Ventrikels beim Kaninchen in elektronenmikroskopischer Betrachtung. Z. Zellforsch. 74, 1–11 (1966).
Leonhardt, H.: Zur Frage einer intraventrikulären Neurosekretion. Eine bisher unbekannte nervöse Struktur im IV. Ventrikel des Kaninchens. Z. Zellforsch. 79, 172–184 (1967).
Leonhardt, H.: Bukettförmige Strukturen im Ependym der Regio hypothalamica des III. Ventrikels beim Kaninchen. Zur Neurosekretions- und Rezeptorenfrage. Z. Zellforsch. 88, 297–317 (1968).
Leonhardt, H.: Ependym. In: Zirkumventrikuläre Organe und Liquor (Bericht über das Symposium in Schloß Reinhardsbrunn, 1968), S. 177–190, Herausgeg. von G. Sterba. Jena: VEB Gustav Fischer 1969.
Leonhardt, H., Eberhardt, H.G.: Dye transport from the median eminence to the hypothalamic wall. A model. In: Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich 1971, p. 335–341. Basel: Karger 1972.
Leveque, T.F., Stutinsky, F., Porte, A., Stoeckel, M.-E.: Morphologie fine d'une différenciation glandulaire du récessus infundibulaire chez le rat. Z. Zellforsch. 69, 381–394 (1966).
Löfgren, F.: New aspects of the hypothalamic control of the adenohypophysis. Acta morph. neerl.-scand. 2, 220–229 (1959).
Löfgren, F.: The influndibular recess, a component in the hypothalamo-adenohypophyseal system. Acta morph. neerl.-scand. 3, 55–78 (1960a).
Löfgren, F.: Hypothalamus-Adenohypophyse, eine hodologische Studie. Acta neuroveg. (Wien) 21, 395–405 (1960b).
Luppa, H., Feustel, G.: Zur Kennzeichnung der Esteraseaktivität in den Tanycyten des Recessus infundibuli der Ratte. Acta histochem. (Jena) 35, 198–199 (1970).
Matsuda, K., Kendall, J.W., Jr., Duyck, C., Greer, M.A.: Neural control of ACTH secretion: effect of acute decerebration in the rat. Endocrinology 72, 845–852 (1963).
Matsui, T.: Fine structure of the median eminence of the rat. J. Fac. Sci. (Tokyo) 4, 71–96 (1966).
Mazzuca, M.: Structure fine des cellules du noyau infundibulaire et de leurs afférences chez le cobaye. J. Microscopie 7, 135–144 (1968).
Mulder, A.H., Geuze, J.J., De Wied, D.: Studies on the subcellular localization of corticotrophin releasing factor (CRF) and vasopressin in the median eminence of the rat. Endocrinology 87, 61–79 (1970).
Nowakowski, H.: Infundibulum und Tuber cinereum der Katze. Dtsch. Z. Nervenheilk. 165, 261–339 (1951).
Oksche, A., Zimmermann, P., Oehmke, H.-J.: Morphometric studies of tubero-eminential systems controlling reproductive functions. In: Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich 1971, p. 142–153. Basel: Karger 1972.
Palkovits, M.: Angaben und Hilfsmittel zur Auswertung von Kernvariationsuntersuchungen Z. mikroskop.-anat. Forsch. 67, 343–355 (1961).
Rinne, U.K.: Ultrastructure of the median eminence of the rat. Z. Zellforsch. 74, 98–122 (1966).
Rinne, U.K.: Effect of adrenalectomy on the ultrastructure and catecholamine fluorescence of the nerve endings in the median eminence of the rat. In: Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich 1971, p. 164–170. Basel: Karger 1972.
Röhlich, P., Vigh, B., Teichmann, I., Aros, B.: Electron microscopy of the median eminence of the rat. Acta biol. Acad. Sci. hung. 15, 431–457 (1965).
Schachenmayr, W.: Über die Entwicklung von Ependym und Plexus chorioideus der Ratte. Z. Zellforsch. 77, 25–63 (1967).
Scharrer, B.: Principles of neuroendocrine communication. In: Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich 1971, p. 3–6. Basel: Karger 1972.
Scharrer, E.: The final common path in neuroendocrine integration. Arch. Anat. micr. Morph. exp. 54, 359–370 (1965).
Scott, D.E., Knigge, K.M.: Ultrastructural changes in the median eminence of the rat following deafferentation of the basal hypothalamus. Z. Zellforsch. 105, 1–32 (1970).
Stöhr, Ph.A.: Über quantitative Veränderungen „gomoripositiver“ Substanzen in Infundibulum und Hypophysenhinterlappen der Ratte nach beidseitiger Adrenalektomie. Z. Zellforsch. 94, 425–433 (1969).
Vigh-Teichmann, I., Vigh, B., Koritsánszky, S., Aros, B.: Liquorkontaktneurone im Nucleus infundibularis. Z. Zellforsch. 108, 17–34 (1970).
Weindl, A., Joynt, R.J.: The median eminence as a circumventricular organ. In: Brainendocrine interaction. Median eminence: Structure and function. Int. Symp. Munich 1971, p. 280–297. Basel: Karger 1972.
Wislocki, G.B., King, L.S.: The permeability of the hypophysis and hypothalamus to vital dyes, with a study of the hypophyseal vascular supply. Amer. J. Anat. 58, 421–472 (1936).
Wittkowski, W.: Zur Ultrastruktur der ependymalen Tanycyten und Pituicyten sowie ihre synaptische Verknüpfung in der Neurohypophyse des Meerschweinchens. Acta anat. (Basel) 67, 338–360 (1967).
Wittkowski, W.: Zur funktionellen Morphologie ependymaler und extraependymaler Glia im Rahmen der Neurosekretion. Elektronenmikroskopische Untersuchungen an der Neurohypophyse der Ratte. Z. Zellforsch. 86, 111–128 (1968).
Wittkowski, W.: Ependymokrinie und Rezeptoren in der Wand des Recessus infundibularis der Maus und ihre Beziehung zum kleinzelligen Hypothalamus. Z. Zellforsch. 93, 530–546 (1969).
Zambrano, D.: The arcuate complex of the female rat during the sexual cycle. An electron microscopic study. Z. Zellforsch. 93, 560–570 (1969).
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Akmayev, I.G., Fidelina, O.V., Kabolova, Z.A. et al. Morphological aspects of the hypothalamic-hypophyseal system. Z.Zellforsch 137, 493–512 (1973). https://doi.org/10.1007/BF00307226
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DOI: https://doi.org/10.1007/BF00307226