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Cellular subsets of the milky spots in the human greater omentum

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Summary

The cellular composition of the human milky spots was investigated on surgically removed specimens of the greater omentum of three 8-month-old infants operated on for neuroblastoma. Monoclonal antibodies and immunohistochemical methods for recognition of macrophages, B-lymphocytes and T-lymphocytes and toluidine-blue staining for mast cells were used. The mean number of cells in one milky spot amounted to 570±33. This cell population was composed of 47.5% macrophages, 29.1% B-lymphocytes, 11.7% T-lymphocytes and 6.1% mast cells. Since inflammation was absent in the material investigated, the numerical data found in the present paper could be regarded as representative cell levels of normal milky spots.

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References

  • Beelen RHJ, Fluitsma DM, Hoefsmit ECM (1980) Peroxidatic activity of monophagocytes developing in omentum milky spots. J reticuloendothel Soc 28:601–609

    Google Scholar 

  • Beelen RHJ, Eestermans IL, Dopp EA, Dijkstra CD (1988) Immunological characteristics of milky spots in the omentum of rats. Adv Exp Med Biol 237:745–750

    Google Scholar 

  • Dux K (1986) Proliferative activity of macrophages in the greater omentum of the mouse in relation to the early postnatal development of the vascular structures. J Leuk Biol 40:445–458

    Google Scholar 

  • Dux K, Janik P, Szaniawska B (1977) Kinetics of proliferation, cell differentiation, and IgM secretion in the omental lymphoid organ of B10/Sn mice following intraperitoneal immunization with sheep erythrocytes. Cell Immunol 32:94–109

    Google Scholar 

  • Dux K, Rouse RV, Kyewski B (1986) Composition of the lymphoid cell population from omental milky spots during the immune response in C57BL/Ka mice. Eur J Immunol 16:1029–1032

    Google Scholar 

  • Mandache E, Moldoveau E, Negoescu A (1987) Lymphatic folliclelike structure in the stimulated omental milky spots. Morphol Embryol 33:285–289

    Google Scholar 

  • Pallesen G, Plesner T (1987) The third international workshop and conference on human leukocyte differentiation antigens with an up-to-date overview of the CD nomenclature. Leukemia 1:231–234

    Google Scholar 

  • Riley JF (1953) The relationship of the mast cells to the blood vessels in the rat. J Path Bact 65:461–469

    Google Scholar 

  • Seifert E (1921) Zur Biologie des menschlichen großen Netzes. Arch Klin Chir 116:510–517

    Google Scholar 

  • Shimotsuma M, Kawata M, Hagiwara A, Takahashi T (1989) Milky spots in the human greater omentum: macroscopic and histological identification. Acta Anat 136:211–216

    Google Scholar 

  • Shimotsuma M, Hagiwara A, Takahashi T, Kawata M, Shields JW (1990) Surface structures and cell zonation in human omental milky spots. Lymphology 23:207–208

    Google Scholar 

  • Suzuki G (1976) Milky spot, as a source of ascitic mast cells in rat. J Kyoto Pref Univ Med 85:679–692

    Google Scholar 

  • Yong LC, Watkins SG, Wilhelm DL (1975) The mast cell: distribution and maturation in the peritoneal cavity of the adult rat. Pathology 7:307–318

    Google Scholar 

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Shimotsuma, M., Takahashi, T., Kawata, M. et al. Cellular subsets of the milky spots in the human greater omentum. Cell Tissue Res 264, 599–601 (1991). https://doi.org/10.1007/BF00319049

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