Summary
The cellular composition of the human milky spots was investigated on surgically removed specimens of the greater omentum of three 8-month-old infants operated on for neuroblastoma. Monoclonal antibodies and immunohistochemical methods for recognition of macrophages, B-lymphocytes and T-lymphocytes and toluidine-blue staining for mast cells were used. The mean number of cells in one milky spot amounted to 570±33. This cell population was composed of 47.5% macrophages, 29.1% B-lymphocytes, 11.7% T-lymphocytes and 6.1% mast cells. Since inflammation was absent in the material investigated, the numerical data found in the present paper could be regarded as representative cell levels of normal milky spots.
References
Beelen RHJ, Fluitsma DM, Hoefsmit ECM (1980) Peroxidatic activity of monophagocytes developing in omentum milky spots. J reticuloendothel Soc 28:601–609
Beelen RHJ, Eestermans IL, Dopp EA, Dijkstra CD (1988) Immunological characteristics of milky spots in the omentum of rats. Adv Exp Med Biol 237:745–750
Dux K (1986) Proliferative activity of macrophages in the greater omentum of the mouse in relation to the early postnatal development of the vascular structures. J Leuk Biol 40:445–458
Dux K, Janik P, Szaniawska B (1977) Kinetics of proliferation, cell differentiation, and IgM secretion in the omental lymphoid organ of B10/Sn mice following intraperitoneal immunization with sheep erythrocytes. Cell Immunol 32:94–109
Dux K, Rouse RV, Kyewski B (1986) Composition of the lymphoid cell population from omental milky spots during the immune response in C57BL/Ka mice. Eur J Immunol 16:1029–1032
Mandache E, Moldoveau E, Negoescu A (1987) Lymphatic folliclelike structure in the stimulated omental milky spots. Morphol Embryol 33:285–289
Pallesen G, Plesner T (1987) The third international workshop and conference on human leukocyte differentiation antigens with an up-to-date overview of the CD nomenclature. Leukemia 1:231–234
Riley JF (1953) The relationship of the mast cells to the blood vessels in the rat. J Path Bact 65:461–469
Seifert E (1921) Zur Biologie des menschlichen großen Netzes. Arch Klin Chir 116:510–517
Shimotsuma M, Kawata M, Hagiwara A, Takahashi T (1989) Milky spots in the human greater omentum: macroscopic and histological identification. Acta Anat 136:211–216
Shimotsuma M, Hagiwara A, Takahashi T, Kawata M, Shields JW (1990) Surface structures and cell zonation in human omental milky spots. Lymphology 23:207–208
Suzuki G (1976) Milky spot, as a source of ascitic mast cells in rat. J Kyoto Pref Univ Med 85:679–692
Yong LC, Watkins SG, Wilhelm DL (1975) The mast cell: distribution and maturation in the peritoneal cavity of the adult rat. Pathology 7:307–318
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Shimotsuma, M., Takahashi, T., Kawata, M. et al. Cellular subsets of the milky spots in the human greater omentum. Cell Tissue Res 264, 599–601 (1991). https://doi.org/10.1007/BF00319049
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DOI: https://doi.org/10.1007/BF00319049