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Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene

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Abstract

The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were made. A partial sequence of the N gene was able to classify our local isolates within three Tospovirus species previously described (Tomato spotted wilt virus, TSWV; tomato chlorotic spot virus, TCSV and groundnut ringspot virus, GRSV). With the sequence data currently available, we performed a cladistic phylogenetic analysis which gave a possible genealogy among members of the Tospovirus genus.

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Authors and Affiliations

  1. Instituto de Bioquímica y Biología Molecular (IBBM), Fac. Cs. Exactas, Universidad Nacional de La Plata, 47 y 115, 1900, La Plata, Argentina

    Dewey Ricardo, Semorile Liliana & Grau Oscar

  2. Departamento de Ciencia y Tecnología y Centro de Estudios e Investigaciones (CEI), Universidad Nacional de Quilmes, Argentina

    Semorile Liliana

  3. Museo de La Plata, 1900, La Plata, Argentina

    Crisci Jorge

  4. Centro de Investigaciones en Ciencias Agropecuarias (CICA), INTA-Castelar, Argentina

    Grau Oscar

  5. Instituto de Microbiología y Zoología Agrícola (IMYZA), CICA, INTA-Castelar, Argentina

    Dewey Ricardo

Authors
  1. Dewey Ricardo
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  2. Semorile Liliana
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  3. Crisci Jorge
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  4. Grau Oscar
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Ricardo, D., Liliana, S., Jorge, C. et al. Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene. Virus Genes 13, 255–262 (1996). https://doi.org/10.1007/BF00366986

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  • DOI: https://doi.org/10.1007/BF00366986

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