Skip to main content
SpringerLink
Log in

Detection of 2,5-hexanedione in the urine of persons not exposed to n-hexane

  • Short Communications
  • Published:
International Archives of Occupational and Environmental Health Aims and scope Submit manuscript

Summary

The neurotoxic n-hexane metabolite 2,5-hexanedione was detected by gas chromatography—mass spectrometry in urine samples from human subjects not exposed to n-hexane or related hydrocarbons. Quantitative determinations by multiple ion detection revealed an amount of 2,5-hexanedione ranging from 0.12 to 0.78 mg/l (0.45 ± 0.20 mg/l; \((0.45 \pm 0.20{\text{mg/l; }}\bar x \pm {\text{SD}}).\) The amounts of 2,5-hexanedione actually measured in urinary extracts depended on the pH employed for acid hydrolysis of the samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Abdel-Rahman MS, Hetland LB, Couri D (1976) Toxicity and metabolism of methyl n-butyl ketone. Am Ind Hyg Assoc J 37:95–102

    PubMed  Google Scholar 

  • Abou-Donia MB, Makkaway HM, Graham DG (1982) The relative neurotoxicities of n-hexane, methyl n-butyl ketone, 2,5-hexanediol, and 2,5-hexanedione following oral or intraperitoneal administration in hens. Toxicol Appl Pharmacol 62:369–389

    Article  PubMed  Google Scholar 

  • Baker TS, Rickert DE (1981) Dose-dependent uptake, distribution, and elimination of inhaled n-hexane in the Fischer-344 rat. Toxicol Appl Pharmacol 61:414–422

    Article  PubMed  Google Scholar 

  • Cianchetti C, Abbritti G, Perticoni G, Siracusa A, Curradi F (1976) Toxic polyneuropathy of shoe-industry workers. A study of 122 cases. J Neurol Neurosurg Psychiatry 39:1151–1161

    PubMed  Google Scholar 

  • De Caprio AP, O'Neill EA (1985) Altertions in rat axonal cytoskeletal proteins induced by in vitro and in vivo 2,5-hexanedione exposure. Toxicol Appl Pharmacol 78:235–247

    Article  PubMed  Google Scholar 

  • DiVincenco GD, Kaplan CJ, Dedinas J (1976) Characterization of the metabolites of methyl n-butyl ketone, methyl iso-butyl ketone, and methyl ethyl ketone in guinea pig serum and their clearance. Toxicol Appl Pharmacol 36:511–522

    Article  PubMed  Google Scholar 

  • Fedtke N, Bolt HM (1985) Methodological investigations on the determination of n-hexane metabolites in urine. Int Arch Occup Environ Health (in press)

  • Gelmont D, Stein RA, Mead JF (1981) Isoprene-the main hydrocarbon in human breath. Biochem Biophys Res Commun 99:1456–1460

    PubMed  Google Scholar 

  • Herskowitz A, Ishii N, Schaumburg H (1971) n-Hexane neuropathy. A syndrome occurring as a result of industrial exposure. New Engl J Med 285:82–85

    PubMed  Google Scholar 

  • Iwata M, Takeuchi Y, Hisanaga N, Ono Y (1983a) A study on biological monitoring of n-hexane exposure. Int Arch Occup Environ Health 51:253–260

    PubMed  Google Scholar 

  • Iwata M, Takeuchi Y, Hisanaga N, Ono Y (1983b) Changes of n-hexane metabolites in urine of rats exposed to various concentrations of n-hexane and its mixture with toluene or MEK. Int Arch Occup Environ Health 53:1–8

    PubMed  Google Scholar 

  • Kivits GAA, Ganguli-Swarttouw MACR, Christ EJ (1981) The composition of alkanes in exhaled air of rats as a result of lipid peroxidation in vivo. Effects of dietary fatty acids, vitamin E and selenium. Biochim Biophys Acta 665:559–570

    Article  PubMed  Google Scholar 

  • Krasavage WJ, O'Donoghue JL, DiVincenco GD, Terhaar CJ (1980) The relative neurotoxicity of methyl n-butyl ketone, n-hexane and their metabolites. Toxicol Appl Pharmacol 52:433–441

    Google Scholar 

  • Mendell JR, Saida K, Ganansia MF, Jackson DB, Weiss H, Gardier RW, Chrisman C, Allan N, Couri D, O'Neill J, Marks B, Hetland L (1974) Toxic polyneuropathy produced by methyl n-butyl ketone. Science 185:787–789

    Google Scholar 

  • Mutti A, Falzoi M, Lucertini S, Arfini G, Zignani M, Lombardi S, Franchini I (1984) n-Hexane metabolism in occupationally exposed workers. Br J Ind Med 41:533–538

    Google Scholar 

  • Perbellini L, Brugnone F, Faggionato G (1981a) Urinary excretion of the metabolites of n-hexane and its isomers during occupational exposure. Br J Ind Med 38:20–26

    PubMed  Google Scholar 

  • Perbellini L, Brugnone F, Silvestri R, Gafturi E (1981b) Measurement of the urinary metabolites of n-hexane, cyclohexane and their isomers by gas chromatography. Int Arch Occup Environ Health 48:99–106

    PubMed  Google Scholar 

  • Sagai M, Ichinose T (1980) Age-related changes in lipid peroxidation as measured by ethane, ethylene, butane and pentane in respired gases of rats. Life Sci 27:731–738

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abteilung Toxikologie und Arbeitsmedizin, Institut für Arbeitsphysiologie an der Universität Dortmund, Ardeystraße 67, 4600, Dortmund, Germany

    N. Fedtke & H. M. Bolt

Authors
  1. N. Fedtke
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. M. Bolt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fedtke, N., Bolt, H.M. Detection of 2,5-hexanedione in the urine of persons not exposed to n-hexane. Int. Arch Occup Environ Heath 57, 143–148 (1986). https://doi.org/10.1007/BF00381382

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00381382

Key words

Search

Navigation