Abstract
A seroepidemiological survey of Toxoplasma gondii infection in sheep and goats was conducted in the Madrid region of Spain. Sera were collected from 60 herds, for which farming management information and other relevant data for their characterization were also obtained through a questionnaire. The seroprevalence was 11.8% (64 out of 541), using the modified (2-mercaptoethanol) direct agglutination technique with a 1:64 cut-off titre. The relationship between seropositivity and the variables in the questionnaire was assessed by multivariate analysis. Four variables were found to be significantly associated with seroprevalence. Two of them, the presence of cats and a previous history of abortion outbreaks in the farm, were factors known to be linked with toxoplasmosis, indicating the validity of the serological data. Seropositivity was also related to a lack of replacements in the preceding year. Proximity to other farms appeared to be a protective factor negatively associated with seropositivity, probably because it was an indicator of proximity to an urban area and the availability of local sanitary facilities.
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Abbreviations
- AIDS:
-
acquired immune deficiency syndrome
- β:
-
logistic regression coefficient
- CI:
-
confidence interval
- MDAT:
-
modified direct agglutination test
- OR:
-
odds ratio
- p :
-
level of significance
- SE:
-
standard error
References
Blewett, D.A., 1983. The epidemiology of ovine toxoplasmosis. I. The interpretation of data for the prevalence of antibody in sheep and other host species. British Veterinary Journal, 139, 537–545
Blewett, D.A. and Watson, W.A., 1984. The epidemiology of ovine toxoplasmosis. III. Observations on outbreaks of clinical toxoplasmosis in relation to possible mechanisms of transmission. British Veterinary Journal, 140, 54–63
Buxton, D., 1991. Toxoplasmosis. In: W.B.Martin and I.D.Aitken (eds), Diseases of Sheep, 2nd edn (Blackwell Scientific, Edinburgh), 49–57
Dubey, J.P., 1994. Toxoplasmosis. Journal of the American Veterinary Medical Association, 205, 1593–1598
Dubey, J.P. and Beattie, C.P., 1988. Toxoplasmosis of Animals and Man (CRC Press, Boca Raton, FL)
Dubey, J.P. and Kirkbride, C.A., 1989. Enzootic toxoplasmosis in sheep in north-central United States. Journal of Parasitology, 75, 673–676
Dubey, J.P. and Kirkbride, C.A., 1990. Toxoplasmosis and other causes of abortions in sheep from north central United States. Journal of the American Veterinary Medical Association, 196, 287–290
Dubey, J.P., Emond, J.P., Desmonts, G. and Anderson, W.R., 1987. Serodiagnosis of postnatally and prenatally induced toxoplasmosis in sheep. American Journal of Veterinary Research, 48, 1239–1243
Hosmer, D.W. and Lemeshow, S., 1989. Model building strategies and methods for logistic regression. Applied Logistic Regression (Wiley, New York)
Kleinbaum, D.G., Kupper, L.L. and Morgenstern, H., 1982. Epidemiologic Research: Principles and Quantitative Methods (Lifetime Learning Publications, Belmont, CA)
Mainar, R.C., Adúriz J.J., de la Cruz, C., Domínguez, L. and Vázquez-Boland, J.A., 1994a. Seroprevalencia frente al virus de la Border Disease en pequeños rumiantes de la Comunidad de Madrid; posible impacto sobre la prolificidad. XIX Jornadas Cientificas de la SEOC, Burgos (Spain), 1994. (Sociedad Española de Ovinotecnia y Caprinotecnia), Abstracts book, p.60
Mainar, R.C., Cuesta, P., Méndez, I., Asensio, M.A., Dominguez, L. and Vázquez-Boland, J.A., 1994b. Bases para una planificatión en ganadería y sanidad animal: caracterización de la explotacíon ovina y caprina de la CAM mediante encuesta y análisis multivariante. XIX Jornadas Cientificas de la SEOC, Burgos (Spain), 1994. (Sociedad Española de Ovinotecnia y Caprinotecnia), Abstracts book, p.92
Martin, W.B., 1984. Epidemiological problems associated with some diseases of sheep. Proceedings of the Society of Veterinary Epidemiology and Preventive Veterinary Medicine, Edinburgh meeting, 1984, pp.1–5
Moreno, T., Martínez-Gómez, F., Hernández-Rodríguez, S., deSetefilla-Martínez-Cruz, M. and Martínez-Moreno, A., 1991. The seroprevalence of ovine toxoplasmosis in Cordoba, Spain. Annals of Tropical Medicine and Parasitology, 85, 287–288
Opel, U., Charleston, W.A.G., Pomroy, W.E. and Rommel, M., 1991. A survey of the prevalence of Toxoplasma infection in goats in New Zealand and a comparison of the latex agglutination and indirect fluorescence tests. Veterinary Parasitology, 40, 181–186
Patton, S., Johnson, S.S. and Puckett, K., 1990. Prevalence of Toxoplasma gondii antibodies in naive populations of dairy goats: compared titers using modified direct agglutination and indirect hemagglutination. Journal of Parasitology, 76, 74–77
Prettejohn, M.W.H., 1988. Sheep and goat health schemes: the ministry approach. Proceedings of the Society of Veterinary Epidemiology and Preventive Medicine, Edinburgh meeting, 1988, pp.93–99
Waltner-Toews, D., Mondesire, R. and Menzies, P., 1991. The seroprevalence of Toxoplasma gondii in Ontario sheep flocks. Canadian Veterinary Journal, 32, 734–737
Wilson, M., Ware, D.A. and Juranek, D.D., 1990. Serologic aspects of toxoplasmosis. Journal of the American Veterinary Medical Association, 196, 277–280
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Mainar, R.C., De La Cruz, C., Asensio, A. et al. Prevalence of agglutinating antibodies to Toxoplasma gondii in small ruminants of the Madrid region, Spain, and identification of factors influencing seropositivity by multivariate analysis. Vet Res Commun 20, 153–159 (1996). https://doi.org/10.1007/BF00385636
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DOI: https://doi.org/10.1007/BF00385636