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A successive histochemical staining for succinate dehydrogenase and “reversed”-ATPase in a single section for the skeletal muscle fibre typing

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Summary

A procedure is described which simplifies the classification of skeletal muscle fibres in that it allows a simultaneous evaluation of both the oxidative capacity and the intensity of “reversed” ATPase of the fibres, and thus enables to distinguish three fibre types — SO, FOG and FG — in one tissue section. After preincubation at pH 4.1–4.2 the cryostat section is incubated for succinate dehydrogenase (SDH) and subsequently for “reversed”-ATPase. This is followed by the fixation with neutral buffered formaldehyde. The results of typing of chicken, minipig and rabbit fibres in a single muscle section stained with this technique are identical to those obtained with the usual method based on a comparison of serial sections of which one is stained for SDH activity the other for “reversed”-ATPase activity.

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  1. Institute of Animal Physiology and Genetics, Czechoslovak Academy of Sciences, CS-277 21, Liběchov, Czechoslovakia

    V. Horák

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  1. V. Horák
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Horák, V. A successive histochemical staining for succinate dehydrogenase and “reversed”-ATPase in a single section for the skeletal muscle fibre typing. Histochemistry 78, 545–553 (1983). https://doi.org/10.1007/BF00496207

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  • DOI: https://doi.org/10.1007/BF00496207

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