Skip to main content
SpringerLink
Log in

A method for the comparison of morphometrical data on skeletal muscles in young rats of different ages and body weights

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

Training affects the properties of skeletal muscles as well as the body weight. Furthermore, the muscle weight and the cross-sectional area of the muscle fibers are related to the body weight. A method is therefore described which eliminates the influence of the body weight on the properties of skeletal muscles and thus allows the real training effects to be studied even if the animals to be compared are of different body weights. It is suggested that this method is useful also for morphometrical studies on organs other than muscle and on experimental situations other than training, e.g. for studies on growth.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barnard, R. J., Edgerton, V. R., Peter, J. B.: Effect of exercise on skeletal muscle—I. Biochemical and histochemical properties. J. appl. Physiol. 28, 762–766 (1970)

    Google Scholar 

  • Bloor, C. M., Leon, A. S.: Interaction of age and exercise on the heart and its blood supply. Lab. Invest. 22, 160–165 (1970)

    Google Scholar 

  • Crews, E. L. III, Fuge, K. W., Oscai, L. B., Holloszy, J. O., Shank, R. E.: Weight, food intake, and body composition: Effects of exercise and of protein deficiency. Amer. J. Physiol. 216, 359–363 (1969)

    Google Scholar 

  • Edgerton, V. R., Barnard, R. J., Peter, J. B., Gillespie, C. A., Simpson, D. R.: Overloaded skeletal muscles of a nonhuman primate (Galago senegalensis). Exp. Neurol. 37, 322–339 (1972)

    Google Scholar 

  • Exner, G. U., Staudte, H. W., Pette, D.: Isometric training of rats—effects upon fast and slow muscle and modification by an anabolic hormone (Nandrolone Decanoate).—I. Female rats. Pflügers Arch. 345, 1–14 (1973)

    Google Scholar 

  • Faulkner, J. A., Maxwell, L. C., Brook, D. A., Lieberman, D. A.: Adaptation of guinea pig plantaris muscle fibers to endurance training. Amer. J. Physiol. 221, 291–297 (1971)

    Google Scholar 

  • Kraus, H., Kirsten, R., Wolff, J. R.: Die Wirkung von Schwimm- und Lauftraining auf die celluläre Funktion und Struktur des Muskels. Pflügers Arch. 308, 57–79 (1969)

    Google Scholar 

  • Lieberman, D. A., Maxwell, L. C., Faulkner, J. A.: Adaptation of guinea pig diaphragm muscle to aging and endurance training. Amer J. Physiol. 222, 556–560 (1972)

    Google Scholar 

  • Maher, J. T., Goodman, A. L., Bowers, W. D., Hartley, L. H., Angelakos, E. T.: Myocardial function and ultrastructure in chronically hypoxic rats. Amer. J. Physiol. 223, 1029–1033 (1972)

    Google Scholar 

  • Müller, W.: Temporal progress of muscle adaptation to endurance training in hind limb muscles of young rats.—A histochemical and morphometrical study. Cell Tiss. Res. 156, 61–87 (1975a)

    Google Scholar 

  • Müller, W.: Isometric training of young rats—Effects upon hind limb muscles. Histochemical, morphometrical, and electron microscopical studies. Manuscript in preparation (1975b)

  • Pattengale, P. K., Holloszy, J. O.: Augmentation of skeletal muscle myoglobin by a program of treadmill running. Amer. J. Physiol. 213, 783–785 (1967)

    Google Scholar 

  • Peter, J. B.: Histochemical, biochemical, and physiological studies of skeletal muscle and its adaptation to exercise. In: Contractility of muscle cells and related processes. R. J. Podolsky, ed., p. 151–173. Englewood-Cliffs, N.J.: Prentice-Hall 1971

    Google Scholar 

  • Pitts, G. C., Bull, L. S., Wakefield, J. A.: Exercise with force feeding in the rat. Amer. J. Physiol. 227, 341–344 (1974)

    Google Scholar 

  • Reitsma, W.: Some structural changes in skeletal muscles of the rat after intensive training. Acta morph. neerl. scand. 7, 229–246 (1970)

    Google Scholar 

  • Tomanek, R. J., Cooper, R. R.: Ultrastructural changes in tenotomized fast- and slowtwich muscle fibers. J. Anat. (Lond.) 113, 409–424 (1972)

    Google Scholar 

  • Tomanek, R. J., Woo, Y. K.: Compensatory hypertrophy of the plantaris muscle in relation to age. J. Geront. 25, 23–29 (1970)

    Google Scholar 

  • Weibel, E. R.: Stereological principles for morphometry in electron microscopic cytology. Int. Rev. Cytol. 26, 235–302 (1969)

    Google Scholar 

  • Zíka, K., Lojda, Z., Kučera, M.: Activities of some oxidative and hydrolytic enzymes in musculus biceps brachii of rats after tonic stress. Histochemie 35, 153–164 (1973)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Universität Konstanz, Konstanz, Federal Republic of Germany

    Werner Müller

Authors
  1. Werner Müller
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Müller, W. A method for the comparison of morphometrical data on skeletal muscles in young rats of different ages and body weights. Histochemistry 43, 241–248 (1975). https://doi.org/10.1007/BF00499705

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00499705

Keywords

Search

Navigation