Summary
In 6 patients with acute myeloblastic leukaemia, daunorubicin was assayed in leukaemic cells from peripheral blood or bone marrow. The cells were separated from red blood cells and granulocytes by centrifugation on a Ficoll-Isopac gradient. The assay was performed by high performance liquid chromatography. Daunorubicin concentrations in peripheral leukaemic cells, 2 hours after the end of the infusion, were much higher than in plasma, the cell/plasma concentration ratio reaching about 350 and rising to almost 700 at 24 h. At that time, drug concentrations were even higher in the bone marrow leukaemic cells. The value of the assay of daunorubicin in cells as method for monitoring therapy is discussed.
References
Chervinsky DS, Wang JJ (1976) Uptake of adriamycin and daunomycin in L 1210 and human leukemia cells: A comparative study. J Med 7: 63–79
Noël G, Peterson C, Trouet A, Tulkens P (1978) Uptake and subcellular localization of daunorubicin and adriamycin in cultured fibroblasts. Eur J Cancer 14: 363–368
Paul C, Baurain R, Gahrton G, Peterson C (1980) Determination of daunorubicin and its main metabolites in plasma, urine and leukemic cells in patients with acute myeloblastic leukemia. Cancer Lett 9: 263–269
Peterson C, Baurain R, Trouet A (1980) The mechanism for cellular uptake, storage and release of daunorubicin. Biochem Pharmacol 29: 1687–1692
Ritter J, Durand F, Fière D, Brazier JL, Faucon G (1984) Daunorubicine et éléments figurés sanguins. Fixation et métabolisme. Thérapie 39: 131–137
Schneider YJ, Baurain R, Zenebergh A, Trouet A (1979) DNA-binding parameters of daunorubicin and doxorubicin in the conditions used for studying the interaction of anthracycline-DNA complexes with cells in vitro. Cancer Chemother Pharmacol 2: 7–10
Strauss JP, Kitchens RL, Patrizi VW, Frenkel EP (1980) Extraction and quantification of daunorubicin and doxorubicin in tissues. J Chromatogr 221: 139–144
Yamamoto K, Acton EM, Henry DW (1972) Antitumor activity of some derivatives of daunorubicin at the amino and methyl ketone functions. J Med Chem 15: 872–875
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Trillet, V., Lakhal, M., Lang, J. et al. Cellular pharmacokinetics of daunorubicin: Uptake by leukaemic cells in vivo and fate. Eur J Clin Pharmacol 29, 127–129 (1985). https://doi.org/10.1007/BF00547382
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DOI: https://doi.org/10.1007/BF00547382