Summary
The oxidation ofl-pipecolic acid to α-aminoadipic acid was studied in eight species of mammals using an assay system more sensitive than those previously employed. After percoll-gradient fractionation, activity was localized to the mitochondrial-enriched fractions in tissues from rabbit, guinea pig, pig, dog, and sheep, with guinea pig kidney cortex showing greatest specific activity. These results contrast with the peroxisomal oxidation ofl-pipecolic acid observed in macaques and man (Mihalik and Rhead 1989; Mihalik et al. 1989). Rats and mice had undetectable levels of both peroxisomal and mitochondriall-pipecolic acid oxidation. In the rat, peroxisomal oxidation activity was not induced by feeding with either clofibrate or clofibrate andl-pipecolic acid. Thus, among mammals, both the ability to oxidizel-pipecolic acid and the organellar location of this oxidation is species dependent.
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Mihalik, S.J., Rhead, W.J. Species variation in organellar location and activity ofl-pipecolic acid oxidation in mammals. J Comp Physiol B 160, 671–675 (1991). https://doi.org/10.1007/BF00571266
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DOI: https://doi.org/10.1007/BF00571266