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The “in vivo” and “in vitro” CO2-Equilibration curves of blood during acute hypercapnia and hypocapnia

II. Theoretical considerations

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Summary

The slope of the CO2-equilibration curve of arterial blood in the pH-logpCO2 diagram,d logpCO2/dpH, was calculated for “in vivo” condition from “in vitro” data assuming that the blood is diluted with interstitial fluid. This theoretical value is always lower than the measured “in vivo” slope. It is suggested that the buffer capacity of intercellular fibers of the connective tissue plays an important role. — The change of the “in vitro” base excess of blood, ΔBE, with “in vivo”pCO2 and respiratory pH, is derived graphically (from the Siggaard-Andersen pH-logpCO2 nomogram) and theoretically. Nonlinear relations are found in all cases. — The influence of additional non-respiratory pH changes on ΔBE is derived graphically. By use of a nomogram the respiratory and the smaller non-respiratory pH effect can clearly be distinguished and ΔBE can be read for any pH-pCO2 pair.

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  1. Physiologisches Institut der Deutschen Sporthochschule Köln, Carl-diem-Weg, D-5000, Köln 41, Germany

    Dieter Böning

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Böning, D. The “in vivo” and “in vitro” CO2-Equilibration curves of blood during acute hypercapnia and hypocapnia. Pflugers Arch. 350, 213–222 (1974). https://doi.org/10.1007/BF00587800

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  • DOI: https://doi.org/10.1007/BF00587800

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