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A cervical primary afferent input to vestibular nuclei as demonstrated by retrograde transport of wheat germ agglutinin-horseradish peroxidase in the rat

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Summary

Wheat germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) was microiontophoretically injected into the vestibular nuclear complex of the rat. Retrogradely labeled neurons were found in ipsilateral spinal ganglia C2-C3 only if the injection site was in the caudal part of the medial vestibular nucleus (MVN). Injections into rostral parts of the MVN, the superior, lateral and descending vestibular nuclei (SVN, LVN, DVN), the nucleus of the solitary tract (STN) and the reticular formation did not result in spinal ganglion labeling. Thus, the caudal part of the MVN appears to be the main vestibular termination site for rostral cervical primary afferents.

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Abbreviations

Cu:

cuneate nucleus

DVN:

descending vestibular nucleus

ECN:

external cuneate nucleus

g7:

genu of the facial nerve

icp:

inferior cerebellar peduncle

In:

intercalated nucleus

LVN:

lateral vestibular nucleus

mlf:

medial longitudinal fasciculus

MVN:

medial vestibular nucleus

PrH:

prepositus hypoglossi nucleus

Ro:

Roller's nucleus

sol:

solitary tract

SVN:

superior vestibular nucleus

12:

hypoglossal nucleus

References

  • Bakker DA, Richmond FJR, Abrahams VC, Courville J (1985) Patterns of primary afferent termination in the external cuneate nucleus from cervical axial muscles in the cat. J Comp Neurol 241:467–479

    Google Scholar 

  • Barany R (1906) Augenbewegungen durch Thoraxbewegungen ausgelöst. Zentrabi Physiol 20:298–302

    Google Scholar 

  • Brodal P, Dietrichs E, Bjaalie JG, Nordby T, Walberg F (1983) Is lectin-coupled horseradish peroxidase taken up and transported by undamaged as well as by damaged fibers in the central nervous system? Brain Res 278:1–9

    Google Scholar 

  • Cohen LA (1961) Role of eye and neck proprioceptive mechanisms in body orientation and motor coordination. J Neurophysiol 24: 1–11

    Google Scholar 

  • Fredrickson JM, Schwarz D, Kornhuber HH (1965) Convergence and interaction of vestibular and deep somatic afferents upon neurons in the vestibular nuclei of the cat. Acta Otolaryngol (Stockh) 61:168–188

    Google Scholar 

  • Grafstein B, Forman D (1980) Intracellular transport in neurons. Physiol Rev 60:1169–1283

    Google Scholar 

  • Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302

    Google Scholar 

  • Gurd JW (1977) Synaptic membrane glycoproteins: molecular identification of lectin receptors. Biochemistry 16:369–374

    Google Scholar 

  • Illing RB, Wässle H (1979) Visualisation of the HRP reaction product using the polarisation microscope. Neurosci Lett 13:7–11

    Google Scholar 

  • La Vail JH, Margolis TP (1987) The anterograde axonal transport of wheatgerm agglutinin as a model for transcellular transport in neurons. In: R. Sidney, M.A. Bisby (eds) Axonal transport. Alan R. Liss New York, pp 311–326

    Google Scholar 

  • Lindsay KW, Roberts TDM, Rosenberg JR (1976) Asymmetric tonic labyrinth reflexes and their interaction with neck reflexes in the decerebrated cat. J Physiol (Lond) 261:583–601

    Google Scholar 

  • Lipp HP, Schwegler H (1980) Improved transport of horseradish peroxidase after injection with a non-ionic detergent (nonidet P-40) into mouse cortex and observations on the relationship between spread at the injection site and amount of transported label. Neurosci Lett 20:49–54

    Google Scholar 

  • Liu CN (1956) Afferent nerves to Clarke's and the lateral cuneate nuclei in the cat. Arch Neurol Psychiatr 75:67–77

    Google Scholar 

  • Longet FA (1845) Sur les troubles qui surviennent dans l'équilibration, la station et la locomotion des animaux, après la section des parties molles de la nuque. Gaz Med (Paris) 13:565–567

    Google Scholar 

  • Magnus R (1924) Koerperstellung. Springer, Berlin

    Google Scholar 

  • Mehler WR, Rubertone JA (1985) Anatomy of the vestibular nucleus complex. In: Paxinos G (ed) The rat nervous system, Vol 2. Hindbrain and spinal cord. Academic Press, Sydney, pp 185–219

    Google Scholar 

  • Mesulam MM (1978) Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a noncarcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26:106–117

    Google Scholar 

  • Mesulam MM, Rosene DL (1979) Sensitivity in horseradish peroxidase neurohistochemistry: a comparative and quantitative analysis of nine methods. J Histochem Cytochem 27:763–773

    Google Scholar 

  • Neuhuber WL, Zenker W (1989) The central distribution of cervical primary afferents in the rat, with emphasis on proprioceptive projections to vestibular, perihypoglossal and upper thoracic spinal nuclei. J Comp Neurol 280:231–253

    Google Scholar 

  • Paxinos G, Watson CH (1986) The rat brain in stereotaxic coordinates, second edition, Academic Press, Sydney

    Google Scholar 

  • Pfaller K, Arvidsson J (1988) Central distribution of trigeminal and upper cervical primary afferents in the rat studied by anterograde transport of horseradish peroxidase conjugated to wheat germ agglutinin. J Comp Neurol 268:77–92

    Google Scholar 

  • Ranson SW, Davenport HK, Doles EA (1932) Intramedullary course of the dorsal-root fibers of the first three cervical nerves. J Comp Neurol 54:1–12

    Google Scholar 

  • Rubin AM, Liedgren SRC, Milne AC, Young JA, Fredrickson JM (1977) Vestibular and somatosensory interaction in the cat vestibular nuclei. Pfluegers Arch 371:155–160

    Google Scholar 

  • Sawchenko PE, Gerfen CR (1985) Plant lectins and bacterial toxins as tools for tracing neuronal connections. TINS 9:378–384

    Google Scholar 

  • Schwab ME, Javoy-Agid F, Agid Y (1978) Labeled wheat germ agglutinin (WGA) as a new, highly sensitive retrograde tracer in the rat brain hippocampal system. Brain Res 152:145–150

    Google Scholar 

  • Steindler DA (1982) Differences in labeling of axons of passage by wheat germ agglutinin after uptake by cut peripheral nerve versus injections within the central nervous system. Brain Res 250:159–167

    Google Scholar 

  • Trojanowski JQ (1983) Native and derivatized lectins for in vivo studies of neuronal connectivity and neuronal cell biology. J Neurosci Methods 9:185–204

    Google Scholar 

  • Trojanowski JQ, Gonatas NK (1983) A morphometric study of the endocytosis of wheatgerm agglutinin-horseradish peroxidase conjugates by retinal ganglion cells in the rat. Brain Res 272:201–210

    Google Scholar 

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  1. Institute of Anatomy, University of Zürich-Irchel, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland

    S. Bankoul & W. L. Neuhuber

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  1. S. Bankoul
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  2. W. L. Neuhuber
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Bankoul, S., Neuhuber, W.L. A cervical primary afferent input to vestibular nuclei as demonstrated by retrograde transport of wheat germ agglutinin-horseradish peroxidase in the rat. Exp Brain Res 79, 405–411 (1990). https://doi.org/10.1007/BF00608252

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  • DOI: https://doi.org/10.1007/BF00608252

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