Summary
Nine granular cell tumours were investigated with poly- or monoclonal antisera to neurone specific enolase (NSE), glial enolase (GE), S 100 protein, alpha-1-antichymotrypsin, lysozyme, laminin, neurofilament (NF), glial fibrillary acidic protein (GFAP), brain creatine kinase (CK), different cytokeratins (Keratin Dako, PKK1), tissue polypeptide antigen (TPA), carcinoembryonic antigen (CEA), desmin, myoglobin and leukocyte common antigen (LCA), using immunoperoxidase-methods on formalin fixed paraffin embedded sections.
While five tumours from adults show specific cytoplasmic staining for NSE and S 100, three congential tumours, two from the gingiva and one from palatine, show only a weak reaction for NSE, reflecting a possible origin from mature and immature Schwann cells, respectively. However, one subcutaneous tumour from near the clavicule of a ten year old girl differs from the other eight tumours by its specific cytoplasmic staining for alpha-1-antichymotrypsin only, supporting the view that there are granular cell tumours of histiocytic origin. In addition, the five adult NSE-S 100 tumours show strong laminin-immunostaining around the single small or syncytial granular cells, whereas pericellular laminin is not detectable in the histiocytic nor in the three congenital tumours.
None of the tumours shows any staining for lysozyme, epithelial, muscular, leukocyte, neurofilament or glial antigens.
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References
Abrikossoff AI (1926) Über Myome, ausgehend von der quergestreiften willkürlichen Muskulatur. Virchows Arch [Pathol Anat] 260:215–233
Abrikossoff AI (1931) Weitere Untersuchungen über Myoblastenmyome. Virchows Arch [Pathol Anat] 280:723–740
Armin A, Connelly EM, Rowden G (1983) An immunoperoxidase investigation of S-100 protein in granular cell myoblastomas: evidence for Schwann cell derivation. Am J Clin Pathol 79:37–44
Azzopardi JG (1956) Histogenesis of granular-cell “myoblastoma”. J Pathol Bacteriol 71:85–94
Bangle R (1953) An early granular-cell myoblastoma confined within a small peripheral myelinated. nerve. Cancer 6:790–793
Burns J, Dixon AJ, Woods JC (1980) Immunoperoxidase localisation of fibronectin in glomeruli of formalin fixed paraffin processed renal tissue. Histochem 67:73–78
Burston J, John R, Spencer H (1962) “Myoblastoma” of the neurohypophysis. J Pathol Bacteriol 83:455–461
Christ ML, Ozello L (1971) Myogenous origin of a granular cell tumor of the urinary bladder. Am J Clin Pathol 56:736–749
Cicero TJ, Ferendelli JA, Suntzeff V, Moore BW (1972) Regional changes in CNS levels of the S-100 and 14-3-2 proteins during development and aging of the mouse. J Neurochem 19:2119–2125
Dhillon AP, Rode J (1983) Immunohistochemical studies of S 100 protein and other neural characteristics expressed by granular cell tumour. Diagn Histopathol 6:23–28
Dixter CT, Konstat MS, Giunta JL, Schreier E, White GE (1975) Congenital granular-cell tumour of alveolar ridge and tongue. Oral Surg 40:270–277
Ekblom P, Miettinen M, Rapola J, Foidart J-M (1982) Demonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissues. Histochem 75:301–307
Enzinger FM, Lattes R, Torloni H (1969) Histological typing of soft tissue tumours, vol 3. In: International classification of tumours, World Health Organisation, Geneva
Feyrter F (1935) Über eine eigenartige Geschwulstform des Nervengewebes im menschlichen Verdauungsschlauch. Virchows Arch [Pathol Anat] 295:480–501
Fischer B (1928) see discussion Klinge F (1928)
Fisher ER, Wechsler H (1962) Granular cell myoblastoma - a misnomer. Electron microscopic and histochemical evidence concerning its Schwann cell derivation and nature (granular cell Schwannoma). Cancer 15:936–954
Fuhr AH, Krogh PHJ (1972) Congenital epulis of the newborn: centennial review of the literature and a report of a case. J Oral Surg 30:30–35
Fust JA, Custer RP (1949) On the neurogenesis of so-called granular cell myoblastoma. Am J Clin Pathol 19:522–535
Garancis JC, Komorowski RA, Kuzma JF (1970) Granular cell myoblastoma. Cancer 25:542–550
Gerbitz K-D, Deufel T, Summer J, Thallemer J, Wieland OH (1983) Brain specific proteins: creatine kinase BB isoenzyme is cochromatographed during preparation of neuron-specific enolase from human brain. Clin Chim Acta 133:233–239
Gerbitz K-D, Summer J, Thallemer J (1984) Brain-specific proteins: solid-phase immunobioluminiscence assay for neuron-specific enolase in human plasma. Clin Chem 30:382–386
Gilliet F, MacGee W, Stoian M, Delacrétaz J (1973) Zur Histogenese granuliertzelliger Tumoren. Der Hautarzt 24:52–57
Haisken W, Langer E (1962) Die submikroskopische Struktur des sogenannten Myoblastenmyoms (Lipidfibrom, granuläres Neurom). Frankf Z Pathol 71:600–616
Holthöfer H, Miettinen A, Paasivno R, Lehto V-P, Linder E, Alfthan O, Virtanen I (1983) Cellular origin and differentiation of renal carcinomas. A fluorescence microscopic study with kidney-specific antibodies, antiintermediate filament antibodies, and lectins. Lab Invest 49:317–326
Klinge F (1928) Über die sogenannten unreifen, nicht quergestreiften Myoblastenmyome. Verh Dtsch Ges Pathol 23:376–382
Lauche (1928) see discussion Klinge F (1928)
Lauche A (1944) Sind die sog. “Myoblastenmyome” Speicherzellgeschwülste? Virchows Arch [Pathol Anat] 312:335–345
Leroux R, Delarue J (1939) Sur trois cas de tumeurs à cellules granuleuses de la cavité buccale. Bull Assoc Franc Cancer 28:427–447
Matthews JB, Mason GI (1982) Oral granular cell myoblastoma: an immunohistochemical study. J Oral Pathol 11:343–352
McCormack LJ, Hazard JB, Dickson JA (1954) Malignant epithelioid neurilemoma (Schwannoma). Cancer 7:725–728
Meister P, Nathrath W (1981) Immunohistochemical characterization of histiocytic tumours. Diagn Histopathol 4:79–87
Miettinen M, Lehtonen E, Lehtola H, Ekblom P, Lehto V-P, Virtanen I (1984) Histogenesis of granular cell tumour - an immunohistochemical and ultrastructural study. J Pathol 142:221–229
Moscovic EA, Azar HA (1967) Multiple granular cell tumors (“myoblastomas”). Case report with electron microscospic observations and review of the literature. Cancer 20:2032–2047
Nakazato Y, Ishizeki J, Takahashi K, Yamaguchi H (1982) Immunohistochemical localisation of S-100 protein in granular cell myoblastoma. Cancer 49:1624–1628
Nathrath WBJ, Meister P (1982) Lysozyme (muramidase) and alpha-1-antichymotrypsin as immunohistochemical tumour markers. Acta histochem Suppl 25:69–72
Nathrath WBJ, Meyer U, Lamerz R, Bassermann R (1984) Tissue polypeptide Antigen (TPA) and Carcinoembryonales Antigen (CEA) in Mammacarzinomen und ihren axillären Lymphknotenmetastasen. Verh Dtsch Ges Path 68:100–103
Nathrath WBJ, Heidenkummer P, Björklund V, Björklund B (1985) Distribution of tissue polypeptide antigen (TPA) in normal human tissues: immunohistochemical study on unfixed, methanol-, ethanol- and formalin-fixed tissues. J Histochem Cytochem 33:99–109
Neumann E (1871) Ein Fall von congenitaler Epulis. Arch Heilk 12:189–190
Pearse AGE (1950) The histogenesis of granular-cell myoblastoma (? granular-cell perineural fibroblastoma). J Pathol Bacteriol 62:351–362
Permanetter W, Meister P (1984) Distribution of lysozyme (muramidase) and a-1-antichymotrypsin in normal and neoplastic epithelial tissues: a survey. Acta Histochem 74:173–179
Primus FJ, Sharkey RM, Hansen HJ, Goldenberg DM (1978) Immunoperoxidase detection of carcinoembryonic antigen. An overview. Cancer 42:1540–1545
Reibel J, Wewer U, Albrechtsen R (1985) The pattern of distribution of laminin in neurogenic tumors, granular cell tumors, and nevi of the oral mucosa. Acta path microbiol immunol scand Sect A 93:41–47
Rode J, Dhillon AP, Papadaki L (1982) Immunohistochemical staining of granular cell tumour for neurone specific enolase: evidence in support of a neural origin. Diagn Histopathol 5:205–211
Rode J, Dhillon AP, Doran JF, Jackson P, Thompson RJ (1985) PGP 9.5, a new marker for human neuroendocrine tumours. Histopathology 9:147–158
Schmechel D, Marangos PJ, Brightman M (1978) Neurone specific enolase is a marker for peripheral and central neuroendocrine cells. Nature 276:834–836
Schwechheimer K, Möller P, Schnabel P, Waldherr R (1983) Emphasis on peanut lectin as a marker for granular cells. Virchows Arch [Pathol Anat] 399:289–297
Shousha S, Lyssiotis T (1979) Granular cell myoblastoma: positive staining for carcinoembryonic antigen. J Clin Pathol 32:219–244
Skoglund LA, Holst E (1983) Granular cell tumor on the alveolar ridge in an adult patient. Report of a case. Int J Oral Surg 12:60–63
Slootweg P, de Wilde P, Vooijs P, Ramaekers F (1983) Oral granular cell lesions. An immunohistochemical study with emphasis on intermediate-sized filaments proteins. Virchows Arch [Pathol Anat] 402:35–45
Smolle J, Konrad K, Kerl H (1985) Granular cell tumors contain myelin-associated glycoprotein. Virchows Arch [Pathol Anat] 406:1–5
Sobel HJ, Marquet E (1974) Granular cells and granular cell lesions. In: Sommers SC (ed) Pathology annual, vol 9. Appleton-Century-Crofts, New York, pp 43–79
Stefansson K, Wollmann RL (1982) S-100 protein in granular cell tumors (granular cell myoblastomas). Cancer 49:1834–1838
Stefansson K, Wollmann R, Jerkovic M (1982a) S-100 protein in soft-tissue tumors derived from Schwann cells and melanocytes. Am J Pathol 106:261–268
Stefansson K, Wollmann RL, Moore BW (1982b) Distribution of S-100 protein outside the central nervous system. Brain Res 234:309–317
Sternberg (1928): see discussion Klinge F (1928)
Travis J, Garner D, Bowen J (1978) Human alpha-1-antichymotrypsin: purification and properties Biochemistry 17:5647–5656
Tapscott SJ, Bennett GS, Toyama Y, Kleinbart F, Holtzer H (1981) Intermediate filament proteins in the developing chick spinal cord. Develop Biol 86:40–54
Trojanowski JQ, Lee VM-Y (1983) Anti-neurofilament monoclonal antibodies: reagents for the evaluation of human neoplasms. Acta Neuropathol 59:155–158
Vanstapel M-J, Peeters B, Cordell J, Heyns W, De Wolf-Peeters C, Desmet V, Mason D (1985) Production of monoclonal antibodies directed against antigenic determinants common to the alpha- and beta-chain of bovine brain S-100 protein. Lab Invest 52:232–238
Vinores SA, Bonnin JM, Rubinstein LJ, Marangos PJ (1984): Immunohistochemical demonstration of neuron-specific enolase in neoplasms of the CNS and other tissues. Arch Pathol Lab Med 108:536–540
Wegelin C (1947) Die Natur der sog. Myoblastentumoren. Schweiz Z Allg Pathol Bakteriol 10:631–653
Weiser G (1978) Granularzelltumor (Granuläres Neurom Feyrter) and Schwannsche Phagen. Elektronenoptische Untersuchung von 3 Fällen. Virchows Arch [Pathol Anat] 380:49–57
Weiss SW, Langloss JM, Enzinger FM (1983): Value of S-100 protein in the diagnosis of soft tissue tumors with particular reference to benign and malignant Schwann cell tumors. Lab Invest 49:299–308
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This work was supported by a grant from the Wilhelm-Sander-Stiftung (82 007.2)
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Nathrath, W.B.J., Remberger, K. Immunohistochemical study of granular cell tumours. Vichows Archiv A Pathol Anat 408, 421–434 (1986). https://doi.org/10.1007/BF00707699
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DOI: https://doi.org/10.1007/BF00707699