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Effect of corticosteroids on the production of superoxide and hydrogen peroxide and the appearance of chemiluminescence by phagocytosing polymorphonuclear leukocytes

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Abstract

Human polymorphonuclear leukocytes were incubated with either methylprednisolone sodium succinate, hydrocortisone sodium succinate, or distilled water, and then latex spherules were added as target particles for phagocytosis. At low concentrations of these corticosteroids (0.04–0.22 mM), no effect was observed on O2 −· production, H2O2 production, or chemiluminescence. At high concentrations of these steroids (2.7 mM), a significant inhibition was observed in both O2 −· production and H2O2 production. At 2.7 mM, methylprednisolone sodium succinate significantly decreased chemiluminescence, whereas hydrocortisone sodium succinate was without effect on chemiluminescence.

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Authors and Affiliations

  1. Blood Coagulation Laboratory and Medical Division, The Memorial Hospital The Department of Medicine, University of Massachusetts Medical School, 01605, Worcester, Massachusetts

    Peter H. Levine, Judy C. Hardin & Kristine L. Scoon

  2. Department of Biochemistry and Pharmacology, Tufts University School of Medicine, 02111, Boston, Massachusetts

    Norman I. Krinsky

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  1. Peter H. Levine
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  2. Judy C. Hardin
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  3. Kristine L. Scoon
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  4. Norman I. Krinsky
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Levine, P.H., Hardin, J.C., Scoon, K.L. et al. Effect of corticosteroids on the production of superoxide and hydrogen peroxide and the appearance of chemiluminescence by phagocytosing polymorphonuclear leukocytes. Inflammation 5, 19–27 (1981). https://doi.org/10.1007/BF00910776

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