Abstract
The penetration route of ookinetes ofPlasmodium yoelii nigeriensis in the midgut of a mosquito,Anopheles omorii, was investigated by electron microscopy. Within 15–18 h after an infective blood meal, ookinetes could be seen in the midgut lumen in the process of entering the midgut wall, or lodged between the basement membrane and the basal lamina. The morphology of the ookinetes and their transformation into early oocysts were found to be similar to those previously reported. Ookinetes penetrated the midgut wall by the intercellular route; however, the intracellular occurrence of the parasite was also observed. Vacuoles appeared around the penetrating ookinetes during the penetration process, but no change in the electron density of the rhoptry-microneme complex was noted.
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Abbreviations
- bl :
-
Basal lamina
- bm :
-
basement membrane
- c :
-
cristalloid
- cj :
-
cellular junction
- d :
-
ductuli
- dm :
-
disrupted mitochondrion
- f :
-
pedicular fold
- ic :
-
intestinal content
- im :
-
intercellular membrane
- l :
-
labyrinth
- mtoc :
-
microtubule organizing centre
- mc :
-
midgut cell
- mv :
-
microvilli
- n :
-
nucleus
- o :
-
ookinete
- ow :
-
oocyst wall
- p :
-
pigment bar
- pm :
-
peritrophic membrane
- rm :
-
rhoptry-microneme complex
- v :
-
vacuole
References
Aikawa M (1988) Fine structure of the malaria parasites. In: Wernsdorfer WH, McGregor I (eds) Principle and practice of malariology. Churchill Livingstone, Edinburgh, pp 97–129
Arakawa R, Nakamura M, Syafruddin, Kamimura K, Ichimori K, Kurihara T (1989) Experimental transmission ofPlasmodium yoelii nigeriensis byAnopheles omorii (Diptera: Culicidae). Jpn J Sanit Zool 40:213–215
Billingsley PF (1990) The midgut ultrastructure of hematophagous insects. Anu Rev Entomol 35:219–248
Canning EU, Sinden RE (1973) The organization of the ookinete stage ofPlasmodium berghei and its transformation into an oocyst. Parasitology 67:29–40
Davies EE (1974) Ultrastructural studies on the early ookinete stage ofPlasmodium berghei nigeriensis and its transformation into an oocyst. Ann Trop Med Parasitol 68:283–290
Garnham PCC, Bird RG, Baker JR (1962) Electron microscope studies of the motile stage of malaria parasites: III. The ookinete ofHaemanoeba andPlasmodium. Trans R Soc Trop Med Hyg 56:187–194
Garnham PCC, Bird RG, Baker JR, Desser SS, El-Nahal HMS (1969) Electron microscopic studies on the motile stage of malaria parasites: VI. The ookinete ofPlasmodium berghei yoelii and its transformation into the early oocyst. Trans R Soc Trop Med Hyg 63:187–194
Maier WA, Becker-Feldmann H, Seitz HM (1987) Pathology of malaria-infected mosquitoes. Parasitol Today 3:216–218
Mehlhorn H, Peters W, Haberkorn A (1980) The formation of kinetes and oocysts inPlasmodium gallinaceum (Haemosporidia) and consideration on phylogenetic relationships between Haemosporidia, Piroplasmida and other coccidia. Protistologica 15:135–154
Meis JFGM, Ponnudurai T (1987) Ultrastructural studies on the interaction ofPlasmodium falciparum with the midgut epithelium ofAnophele stephensi mosquitoes. Parasitol Res 73:500–506
Meis JFGM, Pool G, Gemert GJ van, Lensen AHW, Ponnudurai T, Meuissen JHET (1989)Plasmodium falciparum ookinetes migrate intercellularly throughAnopheles stephensi midgut epithelium. Parasitol Res 76:13–19
Sinden RE (1984) The biology ofPlasmodium in the mosquito. Experientia 40:1330–1343
Stohler H (1957) Analyse des Infektionsverlaufes vonPlasmodium gallinaceum im Darme vonAedes aegypti. Acta Trop (Basel) 18:263–266
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Syafruddin, Arakawa, R., Kamimura, K. et al. Penetration of the mosquito midgut wall by the ookinetes ofPlasmodium yoelii nigeriensis . Parasitol Res 77, 230–236 (1991). https://doi.org/10.1007/BF00930863
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DOI: https://doi.org/10.1007/BF00930863