Summary
Changes in erythrocyte carbonic anhydrase (RBC-CA) activity due to physical exercise were investigated using a measuring apparatus based on the electrometric method.
-
1)
RBC-CA activities were slightly changed due to transient aerobic (20-min run) or anaerobic (200-m run) exercise in moderately trained subjects, but there were no fixed trends.
-
2)
In 3 weeks of weight training, there were no differences in RBC-CA activities between before training and after 1 day of recovery in both trained and untrained subjects. However, after 1 week of recovery, RBC-CA activities slightly decreased in both types of subjects. The trained subjects showed higher levels of RBC-CA activity than the untrained subjects before and after training.
-
3)
The rest values of RBC-CA activity were higher in trained subjects than in untrained subjects. It was evident that RBC-CA activities were higher in subjects (especially in long-distance runners, swimmers etc.) who had undergone long-term strenuous aerobic training.
Similar content being viewed by others
References
Anker N, Mondrup M (1977) Carbonic anhydrase isoenzyme B in erythrocytes of anemic and non-anemic uremic subjects. Clin Chim Acta 79: 341–347
Clark AM, Perrin DD (1951) A re-investigation of the question of activators of carbonic anhydrase. Biochem J 48: 495–503
Davis RP (1963) The measurement of carbonic anhydrase activity. In: Glick D (ed) Methods of biochemical analysis. Interscience, New York, 11: 307–327
Eng L-IL, Hollander L, Fudenberg HH (1967) Carbonic anhydrase and fetal hemoglobin in thyrotoxicosis. Blood 30: 442–448
Funakoshi S, Deutsch HF (1970) Human carbonic anhydrases. III. Immunochemical studies. J Biol Chem 245: 2852–2856
Jones NL, Jurkowski JE (1979) Body carbon dioxide storage capacity in exercise. J Appl Physiol: Respir Environ Exercise Physiol 46: 811–815
Keilin D, Mann T (1941) Activity of carbonic anhydrase within red blood corpuscles. Nature 148: 493–496
Little NG (1980) Erythrocyte carbonic anhydrase activity and physical work capacity. J Sports Med 20: 239–243
Lönnerholm G (1980) Carbonic anhydrase in the lung. Acta Physiol Scand 108: 197–199
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275
Maren TH (1963) The relation between enzyme inhibiton and physiological response in the carbonic anhydrase system. J Pharmacol Exp Ther 139: 140–153
Maren TH (1967) Carbonic anhydrase: chemistry, physiology, and inhibition. Physiol Rev 47: 595–781
McIntosh JEA (1968) Assay of carbonic anhydrase by titration at constant pH. Biochem J 109: 203–207
Meldrum NU, Roughton FJW (1933) Carbonic anhydrase. Its preparation and properties. J Physiol 80: 113–142
Mondrup M, Anker N (1974) Carbonic anhydrase isoenzyme B in the erythrocytes of uremic subjects. Clin Chim Acta 51: 141–146
Mondrup M, Anker N (1976) Carbonic anhydrase isoenzyme B in erythrocytes of subjects with different types of anemia. Clin Chim Acta 69: 463–469
Moynihan JB (1977) Carbonic anhydrase activity in mammalian skeletal and cardiac muscle. Biochem J 168: 567–569
Ohno H, Taniguchi N, Kondo T, Takakuwa E, Terayama K, Kawarabayashi T (1982) Effect of physical exercise on the specific activity of carbonic anhydrase isozyme in human erythrocytes. Experientia 38: 830–831
Ohno H, Hirata F, Terayama K, Kawarabayashi T, Doi R, Kondo T, Taniguchi N (1983) Effect of short physical exercise on the levels of zinc and carbonic anhydrase isoenzyme activities in human erythrocytes. Eur J Appl Physiol 51: 257–268
Philpot FJ, Philpot JSL (1936) A modified colorimetric estimation of carbonic anhydrase. Biochem J 30: 2191–2193
Rickli EE, Ghazanfar SAS, Gibbons BH, Edsall JT (1964) Carbonic anhydrases from human erythrocytes. J Biol Chem 239: 1065–1078
Ridderstråle Y (1979) Observations on the localization of carbonic anhydrase in muscle. Acta Physiol Scand 106: 239–240
Roughton FJW, Booth VH (1946) The manometric determination of the activity of carbonic anbydrase under varied conditions. Biochem J 40: 309–319
Roughton FJW, Dill DB, Darling RC, Graybiel A, Knehr CA, Talbott JH (1941) Some effects of sulfanilamide on man at rest and during exercise. Am J Physiol 135: 77–87
Scott DA, Mendive JR (1941) Observations on carbonic anhydrase. J Biol Chem 139: 661–674
Van Goor H (1948) Carbonic anhydrase, its properties, distribution and significance for carbon dioxide transport. Enzymologia 13: 73–164
Vasina ID (1952) Ugoinaya angidraza krovi u sportsmenov, v cb. Vrachebnyi kontrol v protsesse sportivnogo sovershenstvovaniya. Fizkultura i Sport. Gosudarstvennoe Izdatelstvo, Moskva pp 75–81
Wasserman K, Whipp BJ, Koyal SN, Beaver WL (1973) Anaerobic threshold and respiratory gas exchange during exercise. J Appl Physiol 35: 236–243
Wilbur KM, Anderson NG (1948) Electrometric and colorimetric determination of carbonic anhydrase. J Biol Chem 176: 147–154
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tokuda, S., Iiboshi, A., Suenaga, M. et al. Changes in erythrocyte carbonic anhydrase activity due to physical exercise. Europ. J. Appl. Physiol. 52, 249–254 (1984). https://doi.org/10.1007/BF01015204
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01015204