Abstract
Preimplantation diagnosis and embryo sexing offer great possibilities in the prevention of human diseases and in the field of animal production. These techniques involve blastomere isolation. Isolated blastomeres can grow in culture and develop as whole embryos. In this paper we describe, at the scanning electron microscope level, the characteristics of the plasma membrane surface of isolated blastomeres obtained from mouse embryos at the two-cell stage and of embryos grown to the 2/4- and 4/8- cell stages and compare them to control embryos grown in vitro. According to our results the in vitro manipulation of these embryos does not affect the surface characteristics of the plasma membrane in the early cleavage stages.
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References
Edwards RG, Hollands P: New advances in human embryology: Implications of the preimplantational diagnosis of genetic disease. Hum Reprod 1988;3:545–556
Nijs M, Camus M, Van Steirtehem AC: Evaluation of different biopsy methods of blastomeres from 2-cell mouse embryos. Hum Reprod 1988;3:999–1003
Gimenez C, Vidal F, Egozcue J: In vitro development of preimplantation mouse embryos derived from isolated blastomeres. Hum Reprod 1990;Abstracts II Joint ESCO-ESHRE Meeting:90
Tarkowski AK: Experiments on development of isolated blastomeres of mouse eggs. Nature 1959;184:1286–1287
Tarkowski AK: Experimental studies on regulation in the development of isolated blastomeres of mouse eggs. Acta Theriol 1959;3:191–267
Allen WR, Pashen RL: Production of monozygotic (identical) horse twins by embryo micromanipulation. J Reprod Fertil 1984;71:607–613
Willadsen SM: A method for culture of micromanipulated sheep embryos and its use to produce monozygotic twins. Nature 1979;277:298–300
Willadsen SM: Micromanipulation of the large domestic species.In Mammalian Egg Transfer, EC Adams (ed). Boca Raton, FL, CRC Press, 1982, pp 185–210
Thomson RS, Zamboni L: Phagocytosis of supernumerary spermatozoa of the mouse by two-cell mouse embryos. Anat Rec 1974;178:3
Ziomek CA: Cell polarity in the preimplantation mouse embryo.In The Mammalian Preimplantation Embryo (Regulation of Growth and Differentiation in Vitro), BD Bevister (ed). New York, Plenum, 1987, pp 23–41
Reeve WJD, Ziomek CA: Distribution of microvilli on dissociated blastomeres from mouse embryos: Evidence for surface polarization at compaction. J Embryol Exp Morphol 1981;62:339–350
Thomson RS, Smith DM, Zamboni L: Fertilization of mouse ova “in vitro”: An electron microscopic study. Fertil Steril 1974;25:222–249
Johnson MH, Ziomek CA: The foundation of two distinct cell lineages within the mouse morules. Cell 1981;24:27–80
Whittingham DG: Culture of mouse ova. J Reprod Fert (Suppl) 1971;14:7–21
Tsunoda Y, McLaren A: Effect of various procedures on the viability of mouse embryos containing half the normal number of blastomeres. J Reprod Fertil 1983;69:315–322
Tojo H, Ogita Z, Momose Y: Comparison of the “in vitro” development of mouse single blastomeres with and without the zona pellucida. Experientia 1985;41:108–109
Handyside AH: Distribution of antibody- and lectin-binding sites on dissociated blastomeres from mouse morulae: Evidence for polarization at compaction. J Embryol Exp Morphol 1980;69:99–116
Ziomek CA, Johnson MH: Cell surface interaction induces polarization of mouse 8-cell blastomeres at compaction. Cell 1980;21:935–942
Levy BV, Johnson MH, Goodall H, Maro B: The timing of compaction: Control of a major developmental transition in mouse early embryogenesis. J Embryol Exp Morphol 1986;95:213–237
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Ponsà, M., Nogués, C., Vidal, F. et al. Scanning electron microscope (SEM) study of mouse embryos obtained from isolated blastomeres. J Assist Reprod Genet 8, 279–285 (1991). https://doi.org/10.1007/BF01139785
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DOI: https://doi.org/10.1007/BF01139785