Abstract
The question was examined whether protracted asphyxiation is associated with a distinct macrophage subtype pattern in lung tissue. Immunohistochemical preparations of lung specimens were tested with the antibodies MRP8, MRP14, 27E10 and 25F9 in cases of protracted asphyxiation (n = 8) and in “control” groups (hanging:n = 6; peracute deaths:n = 9). MRP8 and MRP14 interstitial cell counts showed a doubling in protracted asphyxiation. Furthermore, clear increases of 27E10 and 25F9 cells (intravascular and interstitial) were found in protracted asphyxiation, both in the intravascular and interstitial compartments. At present the results look promising as to an additional diagnostic criterion for the differential diagnosis between acute and protracted asphyxiation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berg St (1963) Physiologisch-chemische Befunde im Leichenblut als Ausdruck des Todesgeschehens. Dtsch Z Gerichtl Med 54:136–142
Betz P, Beier G, Eisenmenger W (1994) Pulmonary giant cells and traumatic asphyxia. Int J Legal Med 106:258–261
Betz P, Nerlich A, Penning R, Eisenmenger W (1993) Pulmonary giant cells and their significance for the diagnosis of asphyxiation. Int J Legal Med 106:156–159
Bhardwaj R, Zotz C, Zwadlo-Klarwasser G, Roth J, Goebeler M, Mahnke K, Falk M, Meinardus-Hager G, Sorg C (1992) The calcium binding proteins MRPS and MRP14 form a membrane-associated heterodimer in a subset of monocytes/macrophages present in acute but absent in chronic inflammatory lesions. Eur J Immunol 22:1891–1897
Brinkmann B (1978) Vitale Reaktionen in der Lungenstrombahn bei Tod durch Strangulation. Z Rechtsmedizin 81:133–146
Brinkmann B, Püschel K (1981) Die Lunge als Erfolgsorgan der Strangulationsagonie. Z Rechtsmedizin 86:175–194
Hessian PA, Edgeworth J, Hogg N (1993) MRP 8 and MRP 14 two abundant Ca2+-binding proteins of neutrophils and monocytes. J Leukocyte Biol 53:197–204
Janssen W (1963) Riesenzellenbildung bei Erstickung. Dtsch Z Ges Gerichtl Med 54:200–210
Janssen W (1984) Forensic histopathology. Springer, Berlin Heidelberg New-York
Janssen W, Bärtschi G (1964) Vitale und supravitale Reaktionen der Alveolarzellen nach protrahiertem Sauerstoffmangel. Dtsch Z Ges Gerichtl Med 55:47–66
Mahnke K, Bhardwaj R, Sorg C (1995) Heterodimers of the calcium-binding proteins MRP8 and MRP14 are expressed on the surface of human moncytes upon adherence to fibronectin and collagen. Relation to TNF-a, IL-6, and superoxide production. J Leukoc Biol 57:63–71
Nishi K, Rand S, Fechner G, Brinkmann B (1988) Immunohistochemical localization of A, B, H, Le a and Le b antigens in human thymus using avidin-biotin complex technique. Acta Crim Jpn 54:217–223
Odnik K, Cerletti N, Brüggen J, Clerc RG, Tacsay L, Zwadlo G, Gerhards G, Schlegel R, Sorg C (1987) Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis. Nature 330:80–82
Reh H (1965) Die Verfettung der Alveolarepithelien beim plötzlichen Tod. Beitr Gerichtl Med 22:236–242
Roth J, Sorg C (1992) Die kalziumbindenden Proteine MRP8 und MRP14: Zwei neue Marker zur Monozytendifferenzierung in Entzündungen. Atemw Lungenkrkh 18:349–352
Sorg C (1991) Macrophages in acute in chronic inflammation. Chest Suppl 100:173–175
Sunderkötter C, Beil W, Roth J, Sorg C (1991) Cellular events associated with inflammatory angiogenesis in the mouse cornea. Am J Pathol 138:931–939
Zwadlo G, Brüggen J, Gerhards G, Schlegel R, Sorg C (1985a) Two calcium-binding proteins associated with specific stages of myeloid cell differentiation are expressed by subsets of macrophages in inflammatory tissues. Clin Exp Immunol 72:510–515
Zwadlo G, Bröcker E-B, v Bassewitz D-B, Feige U, Sorg C (1985b) A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes. J Immunol 134:1487–1492
Zwadlo G, Schlegel R, Sorg C (1986) A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflamatory tissues. J Immunol 137:512–518
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Du Chesne, A., Brinkmann, B., Cecchi-Mureani, R. et al. Macrophage subtype patterns in protracted asphyxiation. Int J Leg Med 109, 163–166 (1996). https://doi.org/10.1007/BF01225512
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01225512