Summary
Equine infectious anemia (EIA) virus replicated and produced antigen in cultures of horse leukocytes, equine embryonic spleen cells, and a line of equine dermal (ED) cells. A CPE was produced only in horse leukocyte cultures. Presence of the virus in spleen and dermal cell cultures was monitored frequently by inoculating fluids into leukocyte cultures where a CPE was produced or demonstrating specific EIA antigen by double immunodiffusion. Culture fluids from persistently infected equine dermal cells proved the most practicable source of antigen for quantity production purposes. A fraction precipitated from fluids with 8 per cent polyethylene glycol (MW 6000) was treated with diethyl ether to release the antigen. A semi-quantitative evaluation of antigen yield was performed using radial immunodiffusion.
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Malmquist, W.A., Barnett, D. & Becvar, C.S. Production of equine infectious anemia antigen in a persistently infected cell line. Archiv f Virusforschung 42, 361–370 (1973). https://doi.org/10.1007/BF01250717
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DOI: https://doi.org/10.1007/BF01250717