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Immunogenicity of free synthetic peptides corresponding to T helper epitopes of the influenza HA 1 subunit

Induction of virus cross reacting CD 4+ T lymphocytes in mice

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Summary

Four linear synthetic peptides corresponding to residues 12–29, 50–67, 121–138 and 131–147 of the HA 1 subunit of H 3 subtype influenza virus (NT/60/68) were tested for their capacity to elicit in vivo peptide-specific CD 4+ T cells cross reacting with whole virus.

By studying the in vitro peptide proliferative response of lymph node cells from mice sensitized in vivo with free peptides emulsified in complete or incomplete Freund adjuvant, it was found that region 12–29 could be recognized by CD 4+ T lymphocytes in the context of H-2k and H-2b, region 50–67 in association with H-2b and region 121–138 in the context of H-2d MHC molecules. Outbred OF 1 mice could recognize regions 50–67 and 121–138.

Peptides 50–67 and 121–138 are of potential interest for synthetic vaccine design since they induced in BALB/c (peptide 121–138) and OF 1 (both peptides) mice a CD 4+ T cell population that cross reacted with whole virus. The region 50–67 is of particular interest since only few substitutions have been found in this area in natural variants of the H 3 virus subtype.

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  1. Institut de Biologie Moleculaire et Cellulaire du CNRS, Strasbourg, France

    C. Schneider & M. H. V. Van Regenmortel

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  1. C. Schneider
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  2. M. H. V. Van Regenmortel
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Schneider, C., Van Regenmortel, M.H.V. Immunogenicity of free synthetic peptides corresponding to T helper epitopes of the influenza HA 1 subunit. Archives of Virology 125, 103–119 (1992). https://doi.org/10.1007/BF01309631

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  • DOI: https://doi.org/10.1007/BF01309631

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