Skip to main content
SpringerLink
Log in

Epidermal hyperplasias of northern pike(Esox lucius) associated with herpesvirus and C-type particles

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Northern pike from several locations in central Canada were observed to have two different types of hyperplastic epidermal lesions on the body and fins. One type of epidermal hyperplasia consisted of flat lesions, bluish-white in color with a granular or “gritty” appearance. Histological examination of this granular lesion showed the presence of grossly hypertrophied cells surrounded by normal sized epidermal cells. The enlarged nuclei in thin section contained many typical herpesvirus capsids measuring 100 nm in diameter. A large proportion of the cytoplasmic mass consisted of many dark staining inclusions in which were embedded numerous herpes-like virions. For this herpesvirus of pike the name esocid herpesvirus 1 is proposed in keeping with the herpesvirus nomenclature ofRoizman et al. (19).

The second type of epidermal hyperplasia was a smooth convex whitish translucent tissue mass consisting largely of a population of randomly oriented normal sized undifferentiated cells. Electron microscopic examination showed them to be associated with clusters of C-type retrovirus measuring 150 nm in diameter. The formation of these virus particles was by budding from the cytoplasmic membrane into the inter-cellular spaces.

Neither the herpesvirus nor the C-type particles could be isolated in fish cell cultures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Amin, O. M.: Lymphocystis disease in Wisconsin fishes. J. Fish Dis.2, 207–217 (1979).

    Google Scholar 

  2. Barile, M. F.: Mycoplasma contamination of cell cultures. In:Seligson, D., Hsiung, G. D., Green, R. H. (eds.), CRC Handbook Series in Clinical Laboratory Science Section H: Virology and Rickettsiology, Vol. 1, Part 2, 381–406. West Palm Beach: CRC Press 1978.

    Google Scholar 

  3. Buchanan, J. S., Madeley, C. R.: Studies onHerpesvirus scophthalami infection of turbotScophthalamus maximus (L.): ultrastructural observations. J. Fish Dis.1, 283–295 (1978).

    Google Scholar 

  4. Buchanan, J. S., Richards, R. H., Sommerville, C. S., Madeley, C. R.: A herpes-type virus from the turbotScophthalamus maximum (L.). Vet. Rec.102, 527–528 (1978).

    Google Scholar 

  5. Craighead, J. E., Kanich, R. E., Almeida, J. D.: Nonviral microbodies with viral antigenicity produced in cytomegalovirus-infected cells. J. Virol.10, 766–775 (1972).

    Google Scholar 

  6. Dahlberg, J. E.: Retraviridae: RNA tumor viruses. In:Seligson, D., Hsiung, G. D., Green, R. H. (eds.), CRC Handbook Series in Clinical Laboratory Science Section H: Virology and Rickettsiology Vol. 1, Part 1, 323–335. West Palm Beach: CRC Press 1978.

    Google Scholar 

  7. Dalton, A. J., Melnick, J. L., Bauer, H., Bundreau, G., Bentvelzen, P., Belognesi, D., Gallo, R., Graffi, A., Hagenau, F., Heston, W., Huebner, R., Todaro, G., Heine, U. I.: The case for a family of reverse transcriptase viruses: retraviridae. Intervirology4, 201 (1974).

    Google Scholar 

  8. Darlington, R. W., Moss, III. Howard, L.: The envelope of herpesvirus. Prog. Med. Virol.11, 16–45 (1969).

    Google Scholar 

  9. Fong, C. K. Y., Bia, F., Hsiug, G. D., Madore, P., Chang, P. W.: Ultrastructural development of guinea pig cytomegalovirus in cultured guinea pig embryo cells. J. gen. Virol.42, 127–140 (1979).

    Google Scholar 

  10. Iwasaki, Y., Furukawa, T., Plotkin, S., Koprowski, H.: Ultrastructural study on the sequence of human cytomegalovirus infection in human diploid cells. Arch. ges. Virusforsch.40, 311–324 (1973).

    Google Scholar 

  11. Kanich, R. E., Craighead, J. E.: Human cytomegalovirus infection of cultured fibroblasts. I. Cytopathic effects induced by an adapted and a wild strain. Lab. Invest.27, 263–272 (1972).

    Google Scholar 

  12. Kaplan, A. S.: The herpesviruses, 1–739. New York: Academic Press 1973.

    Google Scholar 

  13. Kelly, R. K., Nielsen, O., Mitchell, S. C., Yamamoto, T.: Characterization ofHerpesvirus vitreum isolated from hyperplastic epidermal tissue of walleye,Stizostedion vitreum vitreum (Mitchill). J. Fish Dis.6, 249–260 (1983).

    Google Scholar 

  14. Ljunberg, O.: Epizootiological and experimental studies of skin tumors in northern pike (Esox lucius L.) in the Baltic Sea. In:Homberger, F., Dawe, C. J., Scarpelli, D.G., Wellings, S., Jr. (eds.), Prog. exp. Tumor Res. Vol. 20, 156–165. Basel: Karger 1979.

    Google Scholar 

  15. Ljunberg, O., Lange, J.: Skin tumors of Northern Pike (Esox lucius L). I. Sarcoma in a Baltic pike population. Bull. Off. Int. Epizoot.69, 1007–1022 (1968).

    Google Scholar 

  16. McGavran, M. H., Smith, M. G.: Ultrastructural, cytochemical and microchemical observations on cytomegalovirus (salivary gland virus) infection of human cells in tissue cultures. Expl. Mol. Path.4, 1–10 (1965).

    Google Scholar 

  17. Mulcahy, M. F., O'Rourke, F. J.: Lymphosarcoma in the pike,Esox lucius L. (Pisces, Esocidae) in Ireland. Proc. R. Ir. Acad.63, 103–129 (1963).

    Google Scholar 

  18. Richards, R. H., Buchanan, J. S.: Studies onHerpesvirus scophthalami infection of turbotScophthalamus maximus (L.), histopathological observations: J. Fish Dis.1, 251–258 (1978).

    Google Scholar 

  19. Roizman, B., Carmichael, L. E., Dienhardt, F., de-The, G., Nahimias, A. J., Plowright, W., Rapp, F., Sheldrick, P., Takahashi, M., Wolf, K.: Herpesviridae: definition, provisional nomenclature and taxonomy. Intervirology16 (1981).

  20. Schubert, G. H.: The infective agent in carp pox. Bull. Off. Int. Epizoot.65, 1011–1022 (1966).

    Google Scholar 

  21. Sonstegard, R. A.: Lymphosarcoma in muskellunge. In:Ribelin, W. E., Migaki, G. (eds.), Pathology of Fishes, 907–924. Madison: Univ. Wisconsin Press 1975.

    Google Scholar 

  22. Sonstegard, R. A.: Studies of the etiology and epizootiology ofLymphosarcoma inEsox (Esox lucius L. andEsox masquinongy). In:Homberger, F., Dawe, C. J., Scarpelli, D. G., Wellings, S. R. (eds.), Prog. exp. Tumor Res. Vol. 20, 141–155. Basel: Karger 1976.

    Google Scholar 

  23. Walker, R.: Fine structure of lymphocystis virus of fish. Virology18, 503–505 (1962).

    Google Scholar 

  24. Walker, R., Weissenberg, R.: Conformity of light and electron microscopic studies on virus particle distribution on Lymphocystos tumor cells of fish. Ann. N.Y. Acad. Sci.126, 375–395 (1965).

    Google Scholar 

  25. Winqvist, G., Ljungberg, O., Hellstroem, B.: Skin tumors of Northern Pike (Esox lucius L.) II. Viral particles in epidermal proliferations. Bull. Off. Int. Epizoot.69, 1023–1031 (1968).

    Google Scholar 

  26. Winqvist, G., Ljunberg, O., Ivarsson, B.: Electron microscopy of sarcoma of the northern pike (Esox lucius L.). In:Dutcher, R. M., Chieco-Blanchi, L. (eds.), Unifying Concepts of Leukemia. Bibl. Haemat. No. 39, 26–30. Basel: Karger 1973.

    Google Scholar 

  27. Yamamoto, T., MacDonald, R. D., Gillespie, D. C., Kelly, R. K.: Viruses associated with lymphocystis disease and dermal sarcoma of walleye(Stizostedion vitreum vitreum). J. Fish. Res. Board Can.33, 2408–2419 (1976).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, University of Alberta, Edmonton, Alberta, Canada

    T. Yamamoto

  2. Freshwater Institute, Fisheries and Oceans, Winnipeg, Manitoba, Canada

    R. K. Kelly & O. Nielsen

Authors
  1. T. Yamamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. K. Kelly
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. Nielsen
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

With 15 Figures

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamamoto, T., Kelly, R.K. & Nielsen, O. Epidermal hyperplasias of northern pike(Esox lucius) associated with herpesvirus and C-type particles. Archives of Virology 79, 255–272 (1984). https://doi.org/10.1007/BF01310815

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01310815

Keywords

Search

Navigation