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Experimental infection of human vascular endothelial cells by pathogenic and nonpathogenic hantaviruses

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Summary

To investigate whether the hantaviruses replicate in endothelial cells, we inoculated human umbilical vein endothelial cells with several pathogenic and nonpathogenic strains of hantavirus. Intracytoplasmic, virus-specific granular fluorescence was detected initially at three days postinoculation and nearly 100% of cells contained viral antigen at 10 days. Cytopathic effect or inclusion bodies were not observed. The in vitro demonstration of the susceptibility of endothelial cells to hantaviruses corroborates in vivo findings, and suggests that endothelial cells may serve as target cells in hemorrhagic fever with renal syndrome.

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Authors and Affiliations

  1. Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, U.S.A.

    R. Yanagihara & D. J. Silverman

  2. the Department of Microbiology, University of Maryland, School of Medicine, Baltimore, Maryland, U.S.A.

    R. Yanagihara & D. J. Silverman

Authors
  1. R. Yanagihara
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  2. D. J. Silverman
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Additional information

This work was presented in part at the First International Conference on Hemorrhagic Fever with Renal Syndrome, Seoul, Korea, May 4–6, 1989.

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Yanagihara, R., Silverman, D.J. Experimental infection of human vascular endothelial cells by pathogenic and nonpathogenic hantaviruses. Archives of Virology 111, 281–286 (1990). https://doi.org/10.1007/BF01311063

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  • DOI: https://doi.org/10.1007/BF01311063

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