Skip to main content

Advertisement

SpringerLink
Log in

Analysis of bovine herpes virus-type 1 isolates by restriction endonuclease fingerprinting

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

In an effort to determine whether distinct types of bovine herpesvirus 1 are responsible for causing specific syndromes, the polypeptides and DNA of 93 BHV1 isolates from the province of Alberta as well as vaccine strains and numerous other Canadian, U.S. and European isolates were analyzed by PAGE and restriction endonuclease fingerprinting, respectively. The polypeptide patterns showed only slight variations: only six isolates contained polypeptides that varied from the norm in their molecular weight, or were absent. Although on the basis of endonuclease patterns the isolates could be categorized into three “strain”s and nine “sub-strains”, we were unable to associate any of the strains with specific clinical signs. This suggests that the type of disease caused may be determined more by the route of infection and animal management practices than by the inherent properties of certain types of BHV1. Most of the Albertan isolates were of one BHV1 type and sub-type.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Abinanti, F. F., Plummer, G. J.: The isolation of infectious bovine rhinotracheitis virus from cattle affected with conjunctivitis observations on experimental infection. Amer. J. Vet. Res.22, 13–17 (1961).

    Google Scholar 

  2. Babiuk, L. A., Wardley, R. C., Rouse, B. T.: Defense mechanisms against bovine herpesvirus. Relationship of virus-host cell events to susceptibility to antibody-complement cell lysis. Infect. Immun.12, 958–963 (1975).

    Google Scholar 

  3. Barenfus, M., Delli Quadri, C. A., McIntyre, R. W., Schroeder, R. J.: Isolation of infectious bovine rhinotracheitis virus from calves with meningoencephalitis. J. Amer. Vet. Med. Assoc.143, 725–728 (1963).

    Google Scholar 

  4. Buchman, T. G., Simpson, T., Nosal, C., Roizman, B., Nahmias, A. J.: The structure of herpes simplex virus DNA and its application to molecular epidemiology. Anm. N. Y. Acad. Sci.354, 297–290 (1980).

    Google Scholar 

  5. Chow, T. L., Malello, J. A., Owen, N. W.: Abortion experimentally induced in cattle by infectious bovine rhinotracheitis. J. Amer. Vet. Med. Assoc.144, 1005–1007 (1964).

    Google Scholar 

  6. Dambaugh, T., Raab-Traub, N., Heller, M., Beisel, C., Hummel, M., Cheung, A., Fennewald, S., King, W., Kieff, E.: Variations among isolates of Epstein-Barr virus. Ann. N. Y. Acad. Sci.354, 309–325 (1980).

    Google Scholar 

  7. Dowdle, W. R., Nahmias, A. J., Harwell, R. W., Pauls, F. P.: Association of antigenic type of herpesvirus hominis with site of viral recovery. J. Immunol.99, 974–980 (1976).

    Google Scholar 

  8. Engles, M., Steck, F., Wyler, R.: Comparison of the genomes of infectious bovine rhinotracheitis and infectious pustular vulvovaginitis virus strains by restriction endonuclease analysis. Arch. Virol.67, 169–174 (1981).

    Google Scholar 

  9. Farley, J. E., Skare, I. B., Skare, J.: Inverted repeat sequences in infectious bovine rhinotracheitis virus DNA. Internat. Conference on Human Herpesviruses, Atlanta, U.S.A., 1980.

  10. Gillespie, J. H., McEntee, K., Kendrick, J. W., Wagner, W. C.: Comparison of infectious pustular vulvovaginitis virus with infectious bovine rhinotracheitis virus. Cornell Vet.49, 288–297 (1959).

    Google Scholar 

  11. Gratzek, J. B., Peter, C. P., Ramsey, F. K.: Isolation and characterization of Am. J. Vet. Res.27, 1567–1572 (1966).

    Google Scholar 

  12. Greig, A. S., Bannister, G. L.: Infection of the bovine udder by bovine herpesvirus. Can. J. Comp. Med. Vet. Sci.29, 57–62 (1965).

    Google Scholar 

  13. Homan, E. J., Easterday, B. C.: Isolation of bovine herpesvirus 1 from trigeminal ganglia of clinically normal cattle. Am. J. Vet. Res.41, 1212–1213 (1980).

    Google Scholar 

  14. House, J. A.: Bovine herpesvirus IBR-IPV. Strain differences. Cornell Vet.62, 431–453 (1972).

    Google Scholar 

  15. Huang, E. S., Houng, S. M., Tegtmeier, C. E., Alford, C.: Cytomegaloviruses: Gentic variation of viral genomes. Ann. N. Y. Acad. Sci.354, 332–346 (1980).

    Google Scholar 

  16. Jericho, K. W. F., Darcel, C. le Q.: Response of the respiratory tract of calves kept at controlled climatic conditions to bovine herpesvirus 1 aerosol. Can. J. Comp. Med.42, 156–167 (1978).

    Google Scholar 

  17. Kahrs, R. F., Smith, R. S.: Infectious bovine rhinotracheitis, infectious pustular vulvovaginitis and abortion in a New York dairy herd. J. Amer. Vet. Med. Assoc.146, 217–220 (1965).

    Google Scholar 

  18. Kendrick, J. W., Gillespie, J. H., McEntee, K.: Infectious pustular vulvovaginitis of cattle. Cornell Vet.48, 458–495 (1958).

    Google Scholar 

  19. Kradel, D. D., Solorzano, R. F., Dunne, H. W., Michel, R. L.: Infectious pustular vulvovaginitis in a Pennsylvania dairy herd. Vet. Med.56, 333–336 (1961).

    Google Scholar 

  20. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London)227, 680–684 (1970).

    Google Scholar 

  21. Lonsdale, D. M., Brown, S. M., Land, J., Subak-Sharpe, J. H., Koprowski, H., Warren, K. G.: Variations in herpes simplex virus isolated from human ganglia and a study of clonal variation of HSV 1. Ann. N. Y. Acad. Sci.354, 291–308 (1980).

    Google Scholar 

  22. Madin, S. H., York, C. J., McKercher, D. G.: Isolation of the infectious bovine rhinotracheitis virus. Science124, 721 (1956).

    Google Scholar 

  23. McKercher, D. G.: Studies of the etiological agents of infectious bovine rhinotracheitis and Bläschenausschlag. Am. J. Vet. Res.24, 501–508 (1963).

    Google Scholar 

  24. Misra, V., Hudson, J. B.: Minor base sequence differences between the genomes of two strains of murine cytomegalovirus differing in virulence. Arch. Virol.64, 1–8 (1980).

    Google Scholar 

  25. Misra, V., Blumenthal, R. M., Babiuk, L. A.: Proteins specified by bovine herpesvirus type-1 (infectious bovine rhinotracheitis virus). J. Virol.40, 367–378 (1981).

    Google Scholar 

  26. Pastoret, P. P., Babiuk, L. A., Misra, V., Griebel, P.: Reactivation of temperature sensitive and non-temperature sensitive infectious bovine rhinotracheitis vaccine virus with dexamethasone. Infect. Immun.29, 483–488 (1980).

    Google Scholar 

  27. Steck, F., Huggler, Chr.: Auftreten, Diagnostik und Bekämpfung der infektiösen Rhinotracheitis und pustulösen Vulvovaginitis (IBR/IPV) in der Schweiz. Schweiz Arch. Tierheilk.117, 439–451 (1975).

    Google Scholar 

  28. Straub, O. C., Matheka, M. D., Strohmaier, K.: Die Differenzierung des Virus der Rhinotracheitis (IBR) vom Virus des Bläschenausschlages (IPV) durch die trägerfreie Virus-Zonenelektrophorese in einem Glukose-Dichtegradienten. Zbl. Vet. Med.B11, 565–571 (1964).

    Google Scholar 

  29. Studdert, M. J., Barker, C. A. V., Savon, M.: Infectious pustular vulvovaginitis virus infection of bulls. Amer. J. Vet. Res.25, 303–314 (1964).

    Google Scholar 

  30. Weelemans, G., Leunen, J., Lomba, F., Gouffaux, M.: Le tropisme digestif du virus IBR. I. Ann. Méd. Vét.118, 175–184 (1974).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    Vikram Misra & L. A. Babiuk

  2. Agriculture Canada, Animal Diseases Research Institute, Lethbridge, Alberta, Canada

    C. le Q. Darcel

Authors
  1. Vikram Misra
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. A. Babiuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. le Q. Darcel
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

With 6 Figures

Rights and permissions

Reprints and permissions

About this article

Cite this article

Misra, V., Babiuk, L.A. & Darcel, C.l.Q. Analysis of bovine herpes virus-type 1 isolates by restriction endonuclease fingerprinting. Archives of Virology 76, 341–354 (1983). https://doi.org/10.1007/BF01311201

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01311201

Keywords

Search

Navigation