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Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line

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Summary

Two different cell populations, high- (MARC-145) and low-permissive cell clones (L-1) to porcine reproductive and respiratory syndrome (PRRS) virus, were derived from MA-104 cell line (parent cell: P) by cell cloning. Maximum virus yields in MARC-145, P, and L-1 cell clones were 108.5, 103.5, and 102.5 tissue culture infective dose 50 (TCID50)/0.1 ml, respectively. The MARC-145 cell clone supported replication of all 11 different porcine reproductive and respiratory syndrome virus isolates that were tested. These results indicated that the MARC-145 cells will be useful for PRRS virus replication.

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Authors and Affiliations

  1. U.S. Meat Animal Research Center, USDA, ARS, Clay Center, Nebraska

    H. S. Kim & J. Kwang

  2. Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota

    I. J. Yoon & H. S. Joo

  3. Diagnostic Virology Laboratory, National Veterinary Services Laboratories, USDA, APHIS, Ames, Iowa, USA

    M. L. Frey

Authors
  1. H. S. Kim
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  2. J. Kwang
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  3. I. J. Yoon
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  4. H. S. Joo
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  5. M. L. Frey
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Kim, H.S., Kwang, J., Yoon, I.J. et al. Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line. Archives of Virology 133, 477–483 (1993). https://doi.org/10.1007/BF01313785

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  • DOI: https://doi.org/10.1007/BF01313785

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