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Antigen retrieval, signal amplification and intensification in immunohistochemistry

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Abstract

In this overview we emphasize new methods of improving immunohistochemical results in formaldehyde-fixed tissue samples. The benefit of heat-induced antigen retrieval in demasking of concealed epitopes is demonstrated. We provide guidance on the influence of heat-induced antigen retrieval in commonly applied monoclonal and polyconal antibodies. Moreover, we show the promising methods of signal amplification using biotinylated tyramine and signal intensification of diaminobenzidine reaction products by metallic ions.

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Authors and Affiliations

  1. Institute of Pathology, Medizinische Hochschule Hannover, Konstanty-Gutschow-Strasse 8, D-30625, Hannover, Germany

    Martin Werner & Reinhard von Wasielewski

  2. Division of Cell and Molecular Pathology, Department of Pathology, University of Zürich, Switzerland

    Paul Komminoth

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  1. Martin Werner
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  2. Reinhard von Wasielewski
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  3. Paul Komminoth
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Werner, M., von Wasielewski, R. & Komminoth, P. Antigen retrieval, signal amplification and intensification in immunohistochemistry. Histochem Cell Biol 105, 253–260 (1996). https://doi.org/10.1007/BF01463928

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