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A convenient technique for the dissection of mosquito salivary glands

  • Published:
Journal of tissue culture methods

Summary

A method is described wherebyP. berghei sprorozoites may be obtained from the salivary glands of infected femaleA. stephensi mosquitoes. The method is relatively easy to master, requires minimal handling of biological material, and is done with ordinary laboratory equipment. In addition, the sporozoites are rendered relatively free of contaminating micro-organisms and the entire operation may be performed aseptically.

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References

  1. Shute, P.; Maryon, M. Laboratory technique for the study of malaria. 2nd ed. London: J and A Churchill Ltd.; 1966: 26–28.

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  2. Strome, C. P. A.; DeSantis, P. L.; Beaudoin, R. L. The cultivation of the exoerythrocytic stages ofPlasmodium berghei from sporozoites. In Vitro 15: 531–536; 1979.

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Authors and Affiliations

  1. American Foundation for Biological Research, Biomedical Research Institute, 12111 Parklawn Drive, 20852, Rockville, Maryland, USA

    C. P. A. Strome, P. L. DeSantis & J. L. Leef

  2. Immunoparasitology Department, Naval Medical Research Institute, 20014, Bethesda, Maryland, USA

    R. L. Beaudoin

Authors
  1. C. P. A. Strome
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  2. P. L. DeSantis
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  3. J. L. Leef
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  4. R. L. Beaudoin
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Strome, C.P.A., DeSantis, P.L., Leef, J.L. et al. A convenient technique for the dissection of mosquito salivary glands. Journal of Tissue Culture Methods 6, 9–11 (1980). https://doi.org/10.1007/BF01665897

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  • DOI: https://doi.org/10.1007/BF01665897

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