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Modified enzyme immunoassay to detect hepatitis C virus antibodies in oral fluid

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Abstract

Samples of oral fluid collected from 18 patients seropositive for hepatitis C virus (HCV) and 49 seronegative patients were tested for the presence of HCV antibodies with two modified serum-screening kits. The following sensitivities and specificities were obtained: HCV 3.0 assay, 72% and 98%, respectively, and Monolisa anti-HCV assay, 100% and 100%, respectively. The modified Monolisa assay demonstrated a striking concordance between serum and saliva samples. The use of oral fluids offers a convenient and noninvasive method applicable to HCV epidemiological studies and screening of high-risk groups.

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Authors and Affiliations

  1. Department of Medical Microbiology, Ninewells Hospital, DD1 9SY, Dundee, UK

    P. G. McIntyre & K. Appleyard

  2. Department of Dental Surgery & Periodontology, Dental Hospital, Dundee, UK

    J. Laszlo & G. R. Ogden

Authors
  1. P. G. McIntyre
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  2. J. Laszlo
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  3. K. Appleyard
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  4. G. R. Ogden
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McIntyre, P.G., Laszlo, J., Appleyard, K. et al. Modified enzyme immunoassay to detect hepatitis C virus antibodies in oral fluid. Eur. J. Clin. Microbiol. Infect. Dis. 15, 882–884 (1996). https://doi.org/10.1007/BF01691223

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  • DOI: https://doi.org/10.1007/BF01691223

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