Abstract
The UL52 and UL53 genes of herpes simplex virus type-1 are both located in theBamHI-L DNA fragment, with an overlap of 14 amino acids. An RNase protection experiment was designed to determine the 5′ termini of both the UL52 and UL53 mRNAs. The 5′ end of the UL52 mRNA was found to be located 100 bp upstream of its ATG initiation codon. Surprisingly, the 5′ terminus of the UL53 gene was found to be downstream of its putative initiation codon. Therefore, it was suggested that the translation of the UL53 open reading frame (ORF) starts at an internal initiation codon that is located 55 codons downstream of the putative one. A hybrid selection experiment was performed in which the UL53-specific mRNA was selected from BSC-1 cells infected with HSV-1 KOS and translated in vitro. The translation product of the UL53 message was found to be 32 kD (shorter than the original 37.5 kD ORF). The size of the protein obtained corresponds with the expected translation product starting at the downstream initiation codon. Analysis of the sequence upstream of this initiation codon reveals the presence of a promotor sequence. Therefore, we suggest that the UL53 protein is 54 amino acids shorter than was previously suggested and is located at coordinates 112,341–113,193.
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Pogue-Guile K.L., Lee G.T.Y., Shapira S.K., and Spear M.P., Virology136, 100–109, 1984.
Pogue-Guile K.L. and Spear M.P., Virology157, 67–74, 1987.
Little S.P. and Schaffer P.A., Virology112, 686–702, 1981.
Bond V.C. and Person S., Virology132, 368–376, 1984.
Machuca I., Jackumont B., and Epstein A., Virology150, 117–125, 1986.
Cai W., Gu B., and Person S., J. Virol62, 2596–2604, 1988.
Tognon M., Guansalini R., Romanelli M.G., Mansrvigi R., and Trevisani B., Virus Res18, 135–150, 1990.
Romanelli M.G., Cattozzo E.M., Faggiolio L., and Tognon M., J Gen Virol72, 1991–1995, 1991.
Debroy C., Pederson N., and Person S., Virology145, 36–48, 19485.
Ben-Hur T., Asher Y., Tabor E., Darai G., and Becker Y., Arch Virol96, 117–122, 1947.
Gordon Y., Gilden D.H., Shtram Y., Asher Y., Tabor E., Eellish M., Delvin M., Snipper D., Hadar J., and Becker Y. Arch Virol.76, 39–49, 1983.
Chou J., Kern E.R., Whitley R.J., and Roizman B., Science250, 1262–1266, 1990.
Ackerman M., Chou J. Sarmiento M., Lerner R.A., and Roizman B.J., Gen Virol58, 843–850, 1986.
MacLean A.R., Fareed M.U., Robertson L., Harland J., and Brown M.S. J Gen Virol72, 631–639, 1991.
Moyal M., Berkowitz C., Rösen-Wolff A., Darai G., and Becker Y., Virus Res26, 99–112, 1993.
McGeoch D.J., Dalrymple M.A., Davison A.J., Dolan A., Frame M.C., NcNab D., Perry L.J., Scott J.E., and Taylor P.J., Gen Virol69, 1531–1574, 1988.
Wu C.A., Nelson N.J., McGeoch D.J., and Challberg M.D., J Virol62, 435–443, 1988.
Dodson M.S. and Lehman I.R., Proc Natl Acad Sci USA88, 1105–1109, 1991.
Lowe W.L., Roberts C.T., Lasky S.R., and Le Roith D., Proc Natl Acad Sci USA84, 8946–8950, 1987.
Starksen N.F., Simpson P.C., Bishopric N., Coughlin S.R., Lee W.M., Escopedo J.A., and Williams L.T., Proc Natl Acad Sci USA83, 8348–8350, 1986.
Preston C.M. and McGeoch D.J., J Virol38, 593–605, 1981.
Laemmli U.K. Nature227, 680, 1970.
Zuker M. and Staigler P., Nucleic Acids Res.9, 133–148, 1981.
Devereux J., Haeberli P., and Smithies O., Nucleic Acids Res10, 305–321, 1984.
Ramaswamy R. and Holland T.S., Virology186, 579–587, 1992.
Hutchinson L., Goldsmith K., Snoddy D., Ghosh H., Graham F.L., and Johnson D.C., J Virol66, 5603–5609, 1992.
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Moyal, M., Becker, Y. Analysis of the transcription pattern of HSV-1 UL52 and UL53 genes. Virus Genes 9, 15–21 (1994). https://doi.org/10.1007/BF01703431
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DOI: https://doi.org/10.1007/BF01703431