Skip to main content
SpringerLink
Log in

Effects of human breast fibroblasts on growth and 17β-estradiol dehydrogenase activity of MCF-7 cells in culture

  • Report
  • Published:
Breast Cancer Research and Treatment Aims and scope Submit manuscript

Summary

The effect of conditioned medium from human breast fibroblasts on growth and 17β-estradiol dehydrogenase (E2DH) activity of the MCF-7 human breast cancer cell line has been investigated. Fibroblasts were derived from normal and tumorous (benign and malignant) breast tissue. Conditioned medium from normal derived fibroblasts inhibited the growth of MCF-7 cells, the effect being statistically significant by day 3 of culture. In contrast, conditioned medium from benign and malignant derived fibroblasts significantly enhanced the growth of MCF-7 cells by day 9 of culture. When added to MCF-7 cells for three days, conditioned medium from all three types of fibroblasts increased E2DH activity in the reductive direction (estrone (E1) → estradiol (E2)) 2–3 fold. There was little or no effect on the oxidative direction (E2 → E1). After 12 days, enzyme activity in the reductive direction was still markedly increased, the effect being greatest in conditioned medium from fibroblasts derived from malignant breast tissue, and least in conditioned medium from fibroblasts derived from benign breast tumors. These results demonstrate that human breast fibroblasts may have paracrine influences on neighbouring epithelial cellsin vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Dickson RB, Huff KK, Spencer EM, Lippman ME: Induction of epidermal growth factor-related polypeptides by 17β-estradiol in MCF-7 human breast cancer cells. Endocrinology 118: 138–142, 1985

    Google Scholar 

  2. Dickson RB, McManaway ME, Lippman ME: Estrogen-induced factors of breast cancer cells partially replace estrogen to promote tumor growth. Science 232: 1540–1543, 1986

    PubMed  Google Scholar 

  3. Huff KK, Kaufman D, Gabbay KH, Spencer EM, Lippman ME, Dickson RB: Secretion of an insulin-like growth factor-I-related protein by human breast cancer cells. Cancer Res 46: 4613–4619, 1986

    PubMed  Google Scholar 

  4. Lippman ME, Dickson RB, Bates S, Knabbe C, Huff K, Swain S, McManaway M, Bronzert D, Kasid A, Gelman ELG: Autocrine and paracrine growth regulation of human breast cancer. Breast Cancer Res Treat 7: 59–70, 1986

    PubMed  Google Scholar 

  5. Vignon F, Capony F, Chambon M, Freiss G, Garcia M, Rochefort H: Autocrine growth stimulation of the MCF-7 breast cancer cells by the estrogen-regulated 52 K protein. Endocrinology 118: 1537–1545, 1986

    PubMed  Google Scholar 

  6. Enami J, Enami S, Koga M: Growth of normal and neoplastic mouse mammary epithelial cells in primary culture: stimulation by conditioned medium from mouse mammary fibroblasts. Gann 74: 845–853, 1983

    PubMed  Google Scholar 

  7. McGrath CM: Augmentation of response of normal mammary epithelial cells to estradiol by mammary stroma. Cancer Res 43: 1355–1360, 1983

    PubMed  Google Scholar 

  8. Haslam SZ: Mammary fibroblast influence on normal mouse mammary epithelial cell responses to estrogen in vitro. Cancer Res 46: 310–316, 1986

    PubMed  Google Scholar 

  9. Haslam SZ, Levely ML: Estrogen responsiveness of normal mouse mammary cells in primary cell culture: association of mammary fibroblasts with estrogenic regulation of progesterone receptors. Endocrinology 116: 1835–1844, 1985

    PubMed  Google Scholar 

  10. Horgan K, Jones D, Mansel RE: Mitogenicity of human fibroblastsin vivo for human breast cancer cells. Br J Surg 74: 227–229, 1987

    PubMed  Google Scholar 

  11. Chang SE, Keen J, Birgitte Lane B, Taylor-Papadimitriou J: Establishment and characterization of SV40-transformed human breast epithelial cell lines. Cancer Res 42: 2040–2053, 1982

    PubMed  Google Scholar 

  12. Braunsberg H, Coldham NG, Leake RE, Cowan SK, Wong W: Actions of a progestogen on human breast cancer cells: mechanisms of growth stimulation and inhibition. Eur J Cancer Clin Oncol 23: 563–571, 1987

    PubMed  Google Scholar 

  13. Butler WB, Kelsey WH, Goran N: Effects of serum and insulin on the sensitivity of the human breast cancer cell line MCF-7 to estrogens and antiestrogens. Cancer Res 41: 82–88, 1981

    PubMed  Google Scholar 

  14. Gompel A, Malet C, Spritzer P, Lalardrie JP, Kuttenn F, Mauvais-Jarvis P: Progestin effect on cell proliferation and 17β-hydroxysteroid dehydrogenase activity in normal human breast cells in culture. J Clin Endocrinol Metab 63: 1174–1180, 1986

    PubMed  Google Scholar 

  15. Newton CJ, James VHT: 17β-hydroxysteroid dehydrogenase activity in leiomyoma and myometrium and its relationship to concentrations of oestrone, oestradiol and progesterone throughout the menstrual cycle. J Steroid Biochem 22: 487–493, 1985

    PubMed  Google Scholar 

  16. Bonney RC, Reed MJ, Davidson K, Beranek PA, James VHT: The relationship between 17β-hydroxysteroid dehydrogenase activity and oestrogen concentrations in human breast tumours and the normal breast tissue. Clin Endocrinol 19: 727–739, 1983

    Google Scholar 

  17. Van Landeghem AAJ, Poortman J, Nabuurs M, Thijssen JHH: Endogenous concentration and sub-cellular distribution of estrogens in normal and malignant human breast tissue. Cancer Res 45: 2900–2906, 1986

    Google Scholar 

  18. McNeill JM, Reed MJ, Beranek PA, Bonney RC, Ghilchik MW, Robinson DJ, James VHT: A comparison of the in vivo uptake and metabolism of3H-oestrone and3H-oestradiol by normal breast and breast tumour tissues in postmenopausal women. Int J Cancer 38: 193–196, 1986

    PubMed  Google Scholar 

  19. McNeill JM, Reed MJ, Beranek PA, Newton CJ, Ghilchik MW, James VHT: The effect of epidermal growth factor, transforming growth factor and breast tumour homogenates on the activity of oestradiol 17β-hydroxysteroid dehydrogenase in cultured adipose tissue. Cancer Letters 31: 213–219, 1986

    PubMed  Google Scholar 

  20. Taylor-Papadimitriou J, Shearer M, Stoker MGP: Growth requirements of human mammary epithelial cells in culture. Int J Cancer 20: 903–908, 1977

    PubMed  Google Scholar 

  21. Osborne CK, Hamilton B, Titus G, Livingston RB: Epidermal growth factor stimulation of human breast cancer cells in culture. Cancer Res 40: 2361–2366, 1980

    PubMed  Google Scholar 

  22. Yang J, Guzman R, Richards J, Imagawa W, McCormick K, Nandi S: Growth factor- and cyclic nucleotide- induced proliferation of normal and mammary epithelial cells in primary culture. Endocrinology 107: 35–41, 1980

    PubMed  Google Scholar 

  23. Prudhomme JF, Malet C, Gompel A, Lalardrie JP, Ochoa C, Boue A, Mauvais-Jarvis P, Kuttenn F: 17β-hydroxysteroid dehydrogenase activity in human breast epithelial cell and fibroblast culture. Endocrinology 114: 1483–1489, 1984

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Breast Clinic, St. Mary's Hospital, London, United Kingdom

    N. Shaikh & M. Ghilchik

  2. Neuroendocrine Lab, Neurochirurgische Universitäts Klinik, Schwabachanlag 6 (Kopf Klinikum), D-8520, Erlangen, FRG

    Eric F. Adams

  3. Department of Chemical Pathology, St. Mary's Hospital Medical School, London, United Kingdom

    Eric F. Adams, C. J. Newton, H. Braunsberg, N. Shaikh, M. Ghilchik & V. H. T. James

Authors
  1. Eric F. Adams
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. J. Newton
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Braunsberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. Shaikh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Ghilchik
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. V. H. T. James
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Adams, E.F., Newton, C.J., Braunsberg, H. et al. Effects of human breast fibroblasts on growth and 17β-estradiol dehydrogenase activity of MCF-7 cells in culture. Breast Cancer Res Tr 11, 165–172 (1988). https://doi.org/10.1007/BF01805840

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01805840

Key words

Search

Navigation