Abstract
The biochemical properties of collagens from the periarticular connective tissues of the human knee (ligaments, semitendinosus and gracilis tendons, and the iliotibial band) were investigated in subjects ranging from 20 to 70 years of age. Although the total collagen content of most tissues was more than 90% of the dry weight, the anterior cruciate ligament and the iliotibial band had relatively low collagen contents. There were no significant changes in the tissue collagen content with aging. However, the anterior cruciate ligament and the patellar tendon of the 20-year-old subject had a higher content of soluble collagen than the other ligaments and tendons. The iliotibial band of the 20-year-old subject contained more collagen that was soluble by a combination of salt, sodium citrate extractions and pepsin digestion. Dihydroxylysinonorleucine was the major reducible cross-link of collagen from all the ligaments. The amount of dihydroxylysinonorleucine in the anterior cruciate ligament of the 20-year-old subject was much higher than that in the other ligaments. In contrast, the tendons and the iliotibial band contained a large amount of histidino-hydroxymerodesmosine and hydroxylysinonorleucine, while the patellar tendon was the only tendon with a significant content of dihydroxylysinonorleucine. Hydroxypyridinium non-reducible cross-links were more abundant in collagens from ligaments than in collagens from the other tissues. The cross-link study and the analysis of collagen solubility showed that patellar tendon collagen more closely resembled the collagen from the anterior cruciate ligament than that from periarticular tendons. It was also shown that the anterior cruciate ligament contains relatively immature collagen compared with the other ligaments. Our findings may contribute to an improved understanding of the functions of the ligaments and tendons around the knee joint. In addition, the data may provide some useful pointers that will help in selection of periarticular tissues as appropriate substitutes for ligament replacement.
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References
Alm A, Gillquist J (1974) Reconstruction of the anterior cruciate ligament by using the medial third of the patellar ligament. Treatment and results. Acta Chir Scand 140: 289–296
Amiel D, Frank C, Harwood FL, Fronek J, Akeson WH (1984) Tendons and ligaments: a morphological and biochemical comparison. J Orthop Res 1: 257–265
Amiel D, Kleiner JB, Roux RD, Harwood FL, Akeson WH (1986) The phenomenon of “ligamentization”: anterior cruciate ligament reconstruction with autogenous patellar tendon. J Orthop Res 4: 162–172
Bailey AJ, Shimokomaki MS (1971) Age-related changes in the reducible cross-links of collagen. FEBS Lett 16: 86–88
Bergman I, Loxley R (1963) Two improved and simplified methods for the spectrophotometric determination of hydroxyproline. Anal Chem 35: 1961–1965
Cho KO (1975) Reconstruction of the anterior cruciate ligament by semitendinosus tenodesis. J Bone Joint Surg [Am] 57: 608–612
Clancy WG Jr, Nelson DA, Reider B, Narechania RG (1982) Anterior cruciate ligament reconstruction using one-third of the patellar ligament, augmented by extra-articular tendon transfers. J Bone Joint Surg [Am] 64: 352–359
Collins HR, Hughston JC, DeHaven KE, Bergfeld JA, Evarts CM (1974) The meniscus as a cruciate ligament substitute. J Sports Med 2: 11–21
DiStefano V, Nixon JE, O'Neil R, Davis O (1977) Pes anserinus transfer: an in vivo biomechanical analysis. Am J Sports Med 5: 204–208
Eriksson E (1976) Reconstruction of the anterior cruciate ligament. Orthop Clin North Am 7: 167–179
Eyre DE, Koob TJ, Van Ness KP (1984) Quantitation of hydroxypyridinium cross-links in collagen by high-performance liquid chromatography. Anal Biochem 137: 380–388
Fairbanks G, Steck TL, Wallach DFH (1971) Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10: 2606–2617
Fujii K, Tanzer ML (1974) Age-related changes in the reducible cross-links of human tendon collagen. FEBS Lett 43: 300–302
Fujii K, Kuboki Y, Sasaki S (1976) Aging of human bone and articular cartilage collagen. Changes in the reducible cross-links and their precursors. Gerontology 22: 363–370
Fujii K, Tanzer ML, Nusgens BV, Lapiere CM (1976) Aldehyde content and crosslinking of type III collagen. Biochem Biophys Res Commun 69: 128–134
Fujii K, Kajiwara T, Kurosu H (1979) Effects of vitamin B6 deficiency on the crosslink formation of collagen. FEBS Lett 97: 193–195
Fujii K, Murota K, Tanzer ML (1983) Abnormal collagen synthesis in skeletal muscle of dystrophic chicken. Biochem Biophys Res Commun 111: 933–938
Gas S, Miller E (1978) Distribution and disposition of the collagens in connective tissues. In: Gas S, Miller E (eds) Collagen in the physiology and pathology of connective tissue. Fischer, Stuttgart New York, pp 46–62
Hey Groves EW (1920) The cruciate ligaments of the knee-joint: their function, rupture, and the operative treatment of the same. Br J Surg 7: 505–515
Insall J, Joseph DM, Aglietti P, Campbell RD (1981) Bone-block iliotibial-band transfer for anterior cruciate ligament insufficiency. J Bone Joint Surg [Am] 63: 560–569
Ireland J, Trickey EL (1980) Macintosh tenodesis for anterolateral instability of the knee. J Bone Joint Surg [Br] 62: 340–345
Jones KG (1963) Reconstruction of the anterior cruciate ligament. A technique using the central one-third of the patellar ligament. J Bone Joint Surg [Am] 45: 925–932
Lange JG, McFadden P, Boden K, Amiel D (1993) The ligamentization process: a 4-year case study following ACL reconstruction with a semitendinosus graft. Arthroscopy 9: 149–153
Laurent GJ, Cockerill P, McAnulty RJ, Hastings JR (1981) A simplified method for quantitation of the relative amounts of type I and type III collagen in small tissue samples. Anal Biochem 113: 301–312
Layman DL, McGoodwin EB, Martin GR (1971) The nature of the collagen synthesized by cultured human fibroblasts. Proc Natl Acad Sci USA 68: 454–458
Lipscomb AB, Johnston RK, Snyder RB, Brothers JC (1979) Secondary reconstruction of anterior cruciate ligament in athletes by using the semitendinosus tendon. Preliminary report of 78 cases. Am J Sports Med 7: 81–83
Lipscomb AB, Johnston RK, Snyder RB (1981) The technique of cruciate ligament reconstruction. Am J Sports Med 9: 77–81
McMaster JH, Weinert CR Jr, Scranton P Jr (1974) Diagnosis and management of isolated anterior cruciate ligament tears: a preliminary report on reconstruction with the gracilis tendon. J Trauma 14: 230–235
Nicholas JA, Minkoff J (1978) Iliotibial band transfer through the intercondylar notch for combined anterior instability (ITPT procedure). Am J Sport Med 6: 341–353
Noyes FR, Butler D, Paulos L, Grood ES (1983) Intra-articular cruciate reconstruction. I. Perspectives on graft strength, vascularization, and immediate motion after replacement. Clin Orthop 172: 71–77
Piez K, Eigner EA, Lewis MS (1963) The chromatographic separation and amino acid composition of the subunits of several collagens. Biochemistry 2: 58–66
Rubin AL, Drake MP, Davison PE, Pfahl D, Speakman PT, Schmitt FO (1965) Effects of pepsin treatment on the interaction properties of tropocollagen macromolecules. Biochemistry 4: 181–190
Seyer JM, Hutcheson ET, Kang AH (1976) Collagen polymorphism in idiopathic chronic pulmonary fibrosis. J Clin Invest 57: 1498–1507
Tanzer ML (1968) Intermolecular cross-links in reconstituted collagen fibrils. Evidence for the nature of the covalent bonds. J Biol Chem 243: 4045–4054
Tanzer ML, Housley T, Berube L, Fairweather R, Franzblau C, Gallop PM (1973) Structure of two histidine-containing cross-links from collagen. J Biol Chem 248: 393–402
Tanzer ML (1976) Cross-linking. In: Ramachandran GN, Redd AH (eds) Biochemistry of collagen. Plenum, New York London, pp 137–162
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Fujii, K., Yamagishi, T., Nagafuchi, T. et al. Biochemical properties of collagen from ligaments and periarticular tendons of the human knee. Knee Surg, Sports traumatol, Arthroscopy 2, 229–233 (1994). https://doi.org/10.1007/BF01845593
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DOI: https://doi.org/10.1007/BF01845593