Abstract
The presence of phytoplasma inFragaria ananassa x Duch cv Senga Sengana showing strawberry green petals symptoms was observed by electron microscopy of phloem tissue. No phytoplasmas were found in asymptomatic strawberry plants used as controls. Nucleic acids extracted from these plants were used in nested-PCR assays with primers amplifying 16S rRNA sequences specifie for phytoplasmas. Bands of 1.2 kb were obtained and the subsequent nested-PCR with specific primers and RFLP analyses allowed to classify the detected phytoplasmas in the aster yellows group (16SrI). They belonged to the subgroup I-C of which type strain is clover phyllody phytoplasma.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beakbane AB, Mishra MD, Posnette AF and Slater CHW (1971) Mycoplasma-like organisms associated with chat fruit and rubbery wood diseases of apple,Malus domestica Borkh., compared with those in strawberry with green petal disease. Journal of General Microbiology 66: 55–62
Blattný C jun and Blattný C sen (1959) A virus green petals disease ofFragaria ananassa. Folia Microbiologica 4: 345–350
Converse RH, Adams AN, Barbara DJ, Clark MF, Casper R, Hepp RF, Martin RR, Morris TJ, Spiegel S and Yoshikawa N (1988) Laboratory detection of viruses and mycoplasmalike organisms in strawberry. Plant Disease 72: 744–749
Clark MF, Barbara DJ and Davies DL (1983) Production and characteristics of antisera to Spiroplasma citri and clover-phyllody associated antigens derived from plants. Annals of applied Biology 103: 251–259
Frazier NW and Posnette AF (1957) Transmission and host-range studies of strawberry green petals virus. Annals of applied Biology 45: 580–588
Gundersen DE, Lee I-M, Schaff DA, Harrison NA, Chang CJ, Davis RE, Kingsbury DT (1996) Genomic diversity and differentiation among phytoplasma strains in 16S rRNA groups I (aster yellows and related phytoplasmas) and III (X-disease and related phytoplasmas). International Journal systematic Bacteriology 46: 64–75
Honetslegrová J, Petrzik K, Spak J and Pelikánová J (1994) Comparison of the Czech scrophularia isolate with the Italian anagyris strain of scrophularia mottle virus. Acta Virologica 38: 21–24
Lee I-M, Davis RE and Hiruki C (1991) Genetic interrelatedness among clover proliferation mycoplasmalike organisms (MLOs) and other MLOs investigated by nucleic acid hybridization and restriction fragment length polymorphism analyses. Applied and Environmental Microbiology 57: 3565–3569
Lee I-M, Hammond RW, Davis RE and Gundersen DE (1993) Universal amplification and analysis of pathogen 16SrDNA for classification and identification of mycoplasmalike organisms. Phytopathology 83: 834–842
Lee I-M, Gundersen DE, Hammond RW and Davis RE (1994) Use of mycoplasmalike organism (MLO) group-specific oligonucleotide primers for nested-PCR assays to detect mixed-MLO infections in a single host plant. Phytopathology 84: 559–566
Lee I-M, Bertaccini A, Vibio M and Gundersen DE (1995) Detection of multiple phytoplasmas in perennial fruit trees with decline symptoms in Italy. Phytopathology 85: 728–735
Miculka B (1974) Zelenokvetost mesícního jahodníku. Zahradnické listy 67: 39
Ploaie PG (1981) Mycoplasmalike organisms and plant diseases in Europe. In: Maramorosh K and Harris KF (eds) Plant diseases and vectors: ecology and epidemiology. Vol 3 (pp 61–104) Academic Press, New York
Posnette AF (1953) Green petal, a new virus disease of strawberries. Plant Pathology 2: 17
Posnette AF and Chiykowski LN (1987) Strawberry green petal and similar diseases. In Converse RH (ed) Virus diseases of small fruits AH 631 (pp 34–46) Government Printing Office Washington DC
Schaff DA, Lee I-M and Davis RE (1992) Sensitive detection and identification of mycoplasmalike organisms by polymerase chain reactions. Biochemistry Biophysic Research Communications 186:1503–1509
Vibio M, Bertaccini A, Lee I-M, Davis RE and Clark MF (1996) Differentiation and classification of aster yellows and related European phytoplasmas. Phytopathologia mediterranea 35: 33–42
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HonetŜlegrová, J.F., Vibio, M. & Bertaccinc, A. Electron microscopy and molecular identification of phytoplasmas associated with strawberry green petals in the Czech Republic. Eur J Plant Pathol 102, 831–835 (1996). https://doi.org/10.1007/BF01877052
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01877052