Abstract
Platelet-mediated antibacterial activity against gram-negative microorganisms has been investigated. Data show that human washed platelets possess the ability to partially inhibit growth ofSalmonella typhi Ty-2 (a smooth strain) but not of two rough strains ofSalmonella minnesota (R345-Rb and R595-Re). On the other hand, no antibacterial activity was noted in the presence of the gram-positiveStaphylococcus aureus. This activity is dependent on the length of incubation of platelets with bacteria and apparently is not mediated by platelet soluble factors. Finally, lysis of platelets with Triton X-100 following their incubation with bacteria gives rise to the same degree of antibacterial activity observed in the untreated samples. This implies that the described platelet function does not rely on the possible trapping of bacteria in platelet aggregates.
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References
C. C. Clawson, G. H. R. Rao and J. G. White,Platelet interaction whit bacteria. IV. Stimulation of the release reaction. Am. J. Pathol.81, 411–419 (1975).
S. B. Smith,Platelet in host resistance: in vitro interaction of platelets, bacteria, and polymorphonuclear leukocytes. Blut25, 104–107 (1972).
C. C. Clawson and J. G. White,Platelet interaction with bacteria. II. Fate of bacteria. Am. J. Pathol.65, 381–395 (1971).
R. A. Kahn and L. J. Flinton,The relationship between platelets and bacteria. Blood44, 715–721 (1974).
C. J. Czuprynski and E. Balish,Interaction of rat platelets with Listeria monocytogenes. Infect. Immun.33, 103–108 (1981).
P. N. Walsh,Platelet washing by albumin density gradient separation (ADGS). InPlatelet function and thrombosis. A review of methods (Ed. P. M. Mannucci and S. Gorini). Plenum Press, New York-London 1982.
D. C. Morrison and R. J. Ulevitch,The effects of bacterial endotoxins on host mediation systems. Am. J. Pathol.93, 527–617 (1978).
I. Roitt,Essential Immunology (ed. 2). Blackwell. Oxford (1974).
J. Hawiger, D. K. Hammond, S. Timmons and A. Z. Budzynski,Interaction of human fibrinogen with staphylococci: presence of a binding region on normal and abnormal fibrinogen variants and fibrinogen derivatives. Blood51, 799–812 (1978).
S. J. Klebanoff,Antimicrobial mechanisms in neutrophilic polymorphonuclear leukocytes. Seminar. Hemat.12, 117–142 (1975).
G. F. Springer and J. C. Adye,Endotoxin binding substances from human leucocytes and platelets. Infect. Immun.12, 978–986 (1975).
G. F. Springer and J. C. Adye,Endotoxin interaction with phosphatides from human leukocytes and platelets and with lymphoid cells of susceptible and resistant mice. InMicrobiology (Ed. D. Schlessinger). Am. Soc. Microbiol., Washington 1977.
D. C. Morrison and Z. G. Oades,Mechanisms of lipopolysaccharide-initiated rabbit platelet responses. II. Evidence that lipid A is responsible for binding of lipopolysaccharide to the platelet. J. Immunol.122, 753–758 (1979).
J. Hawiger, A. Hawiger, S. Steckley, S. Timmons and C. Cheng,Membrane changes in human platelets induced by lipopolysaccharide endotoxin. Br. J. Haematol.35, 285–299 (1977).
C. C. Clawson,Platelet interaction with bacteria. III. Ultrastructure. Am. J. Pathol.70, 449–464 (1973).
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Miragliotta, G., Lafata, M. & Jirillo, E. Anti-bacterial activity mediated by human platelets. Agents and Actions 25, 401–406 (1988). https://doi.org/10.1007/BF01965049
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DOI: https://doi.org/10.1007/BF01965049