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Bacterial vaginosis: Microbiological and clinical findings

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Abstract

A prospective study was performed involving 101 women who consecutively attended a primary health care unit for complaints of genital malodour and/or abnormal vaginal discharge. Bacterial vaginosis was diagnosed in 34 women on the basis of four diagnostic criteria: vaginal pH > 4.7, homogeneous vaginal discharge, a positive amine test and clue cells. The sensitivity of these criteria was > 90 % except for homogeneous discharge (82 %). Their specificity was > 90% except for vaginal pH > 4.7 (46%); a specificity of 87% could have been achieved by using the criterion for vaginal pH ⩾ 5.0. There was a strong association between diagnosis of bacterial vaginosis and the concomitant occurrence ofGardnerella vaginalis, Mobiluncusspp. andBacteroidesspp. There was no difference between women with or without bacterial vaginosis as regards contraception methods (except for use of an intrauterine device), age at first intercourse, or earlier episodes of vaginal discharge. Sexual transmission of the predominant bacteria was not supported by data collected from the male consorts.

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References

  1. Blackwell, A., Fox, A. R., Phillips, I., Barlow, D.: Anaerobic vaginosis (non-specific vaginitis): clinical, microbiological, and therapeutical findings. Lancet 1983, ii: 1379–1382.

    Article  Google Scholar 

  2. Eschenbach, D. A.: Vaginal infection. Clinical Obstetrics and Gynecology 1983, 26: 186–202.

    PubMed  CAS  Google Scholar 

  3. Jones, B. M., Geary, I., Alawattagama, A. B., Kinghorn, G. R., Duerden, B. I.: In-vitro and in-vivo activity of metronidazole againstGardnerella vaginalis, Bacteroides spp. andMobiluncus spp. in bacterial vaginosis. Journal of Antimicrobial Chemotherapy 1985, 16: 189–197.

    PubMed  CAS  Google Scholar 

  4. Gardner, H. L., Dukes, C. D.:Haemophilus vaginalis vaginitis. A newly defined specific infection previously classified “nonspecific” vaginitis. American Journal of Obstetrics and Gynecology 1955, 69: 962–976.

    PubMed  CAS  Google Scholar 

  5. Dunkelberg, W. E., Hefner, J. D., Patow, W. E., Wyman, F. J., Orup, H. J.:Haemophilus vaginalis among asymptomatic women. Obstetrics and Gynaecology 1962, 20: 629–632.

    Google Scholar 

  6. McCormack, W. M., Hayes, C. H., Rosner, B., Evrard, J. R., Crockett, V. A., Alpert, S., Zinner, S. H.: Vaginal colonization withCorynebacterium vaginale (Haemophilus vaginalis). Journal of Infectious Diseases 1977, 136: 740–745.

    PubMed  CAS  Google Scholar 

  7. Pheifer, T. A., Forsyth, P. S., Durfee, M. A., Pollock, H. M., Holmes, K. K.: Nonspecific vaginitis. Role ofHaemophilus vaginalis and treatment with metronidazole. New England Journal of Medicine 1978, 298: 1429–1434.

    Article  PubMed  CAS  Google Scholar 

  8. Amsel, R., Totten, P. A., Spiegel, C. A., Chen, K. C. S., Eschenbach, D., Holmes, K. K.: Nonspecific vaginitis: diagnostic criteria and microbial and epidemiological associations. American Journal of Medicine 1983, 74: 14–22.

    Article  PubMed  CAS  Google Scholar 

  9. Spiegel, C. A., Amsel, R., Eschenbach, D., Schoenknecht, F., Holmes, K. K.: Anaerobic bacteria in nonspecific vaginitis. New England Journal of Medicine 1980, 303: 601–607.

    Article  PubMed  CAS  Google Scholar 

  10. Holst, E., Svensson, L., Skarin, A., Weströrn, L., Mårdh, P.-A.: Vaginal colonization withGardnerella vaginalis and anaerobic curved rods. In: Mårdh, P.-A., Taylor-Robinson, D. (ed.): Bacterial vaginosis. Almquist and Wiksell International, Stockholm, 1984, p. 147–152.

    Google Scholar 

  11. Spiegel, C. A., Eschenbach, D. A., Amsel, R., Holmes, K. K.: Curved anaerobic bacteria in bacterial (nonspecific) vaginosis and their response to antimicrobial therapy. Journal of Infectious Diseases 1983, 148: 817–822.

    PubMed  CAS  Google Scholar 

  12. Thomason, J. L., Schreckenberger, P. C., Spellacy, W. N., Riff, L. J., LeBeau, L. J.: Clinical and microbiological characterization of patients with nonspecific vaginosis associated with motile, curved anaerobic rods. Journal of Infectious Diseases 1984, 149: 801–809.

    PubMed  CAS  Google Scholar 

  13. Roberts, M. C., Hillier, S. L., Schoenknecht, F. D., Holmes, K. K.: Comparison of Gram stain, DNA probe, and culture for the identification of species ofMobiluncus in female genital specimens. Journal of Infectious Diseases 1985, 152: 74–77.

    PubMed  CAS  Google Scholar 

  14. Mårdh, P.-A.: Laboratory diagnosis. Bacteria, chlamydiae, mycoplasmas. In: Holmes, K. K., Mårdh, P.-A., Sparling, P. F., Wiesner, P. J. (ed.): Sexually transmitted diseases. McGraw-Hill, Inc., New York, 1984, p. 829–855.

    Google Scholar 

  15. Tokumaru, T.: Herpesviruses. In: Lennette, E. H., Schmidt, N. J. (ed.): Diagnostic procedures. American Public Health Association, Inc., New York, 1969, p. 641–676.

    Google Scholar 

  16. Holdeman, L. V., Cato, E. P., Moore, W. E. C.: Anaerobe laboratory manual. Virginia Polytechnic Institute, Blacksburg, VA, 1977.

    Google Scholar 

  17. Holst, E., Hofmann, H., Mårdh, P.-A.: Anaerobic curved rods in genital samples of women: performance of different selective media, comparison of detection by microscopy and culture studies, and recovery from different sampling sites. In: Mårdh, P.-A., Taylor-Robinson, D. (ed.): Bacterial vaginosis. Almquist and Wiksell International, Stockholm, 1984, p. 117–124.

    Google Scholar 

  18. Holst, E., Skarin, A., Mårdh, P.-A.: Characteristics of anaerobic comma-shaped bacteria recovered from the female genital tract. European Journal of Clinical Microbiology 1982, 1: 310–316.

    PubMed  CAS  Google Scholar 

  19. Totten, P. A., Amsel, R., Hale, P., Piot, P., Holmes, K. K.: Selective differential human blood bilayer media for isolation ofGardnerella (Haemophilus) vaginalis. Journal of Clinical Microbiology 1982, 15: 141–147.

    PubMed  CAS  Google Scholar 

  20. Lodder, J., Kreger-van Rij, N. J. W.: The yeasts. North-Holland Publishing Company, Amsterdam, 1967.

    Google Scholar 

  21. Mårdh, P.-A., Taylor-Robinson, D. (ed.): Bacterial vaginosis. Almquist and Wiksell International, Stockholm, 1984.

    Google Scholar 

  22. Paavonen, J., Miettinen, A., Stevens, C. E., Chen, K. C. S., Holmes, K. K.:Mycoplasma hominis in nonspecific vaginitis. Sexually Transmitted Diseases 1983, 10: 271–275.

    PubMed  CAS  Google Scholar 

  23. Holst, E., Mårdh, P.-A., Thelin, I.: Recovery of anaerobic curved rods andGardnerella vaginalis from the urethra of men, including male hetero-sexual consorts of female carriers. In: Mårdh, P.-A., Taylor-Robinson, D. (ed.): Bacterial vaginosis. Almquist and Wiksell International, Stockholm, 1984, p. 173–177.

    Google Scholar 

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Authors and Affiliations

  1. Department of Medical Microbiology, Sölvegatan 23, 223 62, Lund, Sweden

    E. Holst

  2. Institute of Clinical Bacteriology, University of Uppsala, Uppsala, Sweden

    E. Holst & P. -A. Mårdh

  3. Community Health Centre, Hantverkaregatan, Helsingborg, Sweden

    B. Wathne

  4. Department of Research in Primary Health Care, Dalby, Sweden

    B. Hovelius

Authors
  1. E. Holst
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  2. B. Wathne
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  3. B. Hovelius
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  4. P. -A. Mårdh
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Holst, E., Wathne, B., Hovelius, B. et al. Bacterial vaginosis: Microbiological and clinical findings. Eur. J, Clin. Microbiol. 6, 536–541 (1987). https://doi.org/10.1007/BF02014242

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