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Rabbit and ferret parietal cell inhibition byHelicobacter species

  • Esophageal, Gastric, And Duodenal Disorders
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Abstract

We tested sonicates ofHelicobacter pylori, H. mustelae, andH. felis for inhibition of acid secretion in rabbit and ferret isolated gastric glands. ThreeH. pylori strains, two of threeH. mustelae strains, and twoH. felis strains significantly inhibited acid secretion in rabbit cells by 95.2–93.3%, 55.9% and 96.4%, and 83.4–96%, respectively. AllHelicobacter strains examined inhibited acid secretion by ferret cells by 65.3–76.8%, 89.1–97.6%, and 85.8–92.8%.H. pylori inhibited acid secretion after stimulation with histamine and isobutylmethylxanthine or with 8-bromo-cyclic adenosine monophosphate (P<0.05 for all tests). These findings demonstrate that acid inhibition is a property common to the threeHelicobacter species tested. It occurs independently of the mammalian origin of the parietal cell, and it does not involve blockade of histamine-2 receptors.

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References

  1. Hengels KJ: Relationship betweenHelicobacter pylori and gastroduodenal physiology: Acid secretion and pepsinogens. InHelicobacter pylori, Gastritis, and Peptic Ulcer. P. Malfertheiner, H Ditschuneit (eds). Berlin, Springer-Verlag, 1990, pp 312–316

    Google Scholar 

  2. Morris A, Nicholson G: Ingestion ofCampylobacter pyloridis causes gastritis and raised fasting gastric pH. Am J Gastroenterol 82:192–199, 1987

    PubMed  Google Scholar 

  3. Graham DY, Alpert LC, Smith JL, Yoshimura HH: IatrogenicCampylobacter pylori infection is a cause of epidemic achlorhydria. Am J Gastroenterol 83:974–980, 1988

    PubMed  Google Scholar 

  4. Barthel JS, Westblom TU, Havey AD, Gonzalez F, Everett ED: Gastritis andCampylobacter pylori in healthy, asymptomatic volunteers, Arch Intern Med 148:1149–1151, 1988

    PubMed  Google Scholar 

  5. Ramsey EJ, Carey KV, Peterson WL, Jackson JJ, Murphy FK, Read NW, Taylor K, Trier JS, Fordtran JS: Epidemic gastritis with hypochlorhydria. Gastroenterology 76:1449–1457, 1979

    PubMed  Google Scholar 

  6. Gledhill T, Leicester RJ, Addis B, Lightfoot N, Barnard J, Viney N, Darkin D, Hunt RH: Epidemic hypochlorhydria. Br Med J 290:1383–1386, 1985

    Google Scholar 

  7. Fox JG, Cabot EB, Taylor NS, Laraway R: Gastric colonization byCampylobacter pylori subsp.mustelae in ferrets. Infect Immun 56:2994–2996, 1988

    PubMed  Google Scholar 

  8. Fox JG, Correa P, Taylor NS, Lee A, Otto G, Murphy JC, Rose R:Helicobacter mustelae-associated gastritis in ferrets. An animal model forHelicobacter pylori gastritis in humans. Gastroenterology 99:352–361, 1990

    PubMed  Google Scholar 

  9. Fox JG, Otto G, Taylor NS, Rosenblad W, Murphy JC:Helicobacter mustelae-induced gastritis and elevated gastric pH in the ferret (Mustela putorius furo). Infect Immun 59:1875–1880, 1991

    PubMed  Google Scholar 

  10. Radin MJ, Eaton KA, Krakowka S, Morgan DR, Lee A, Otto G, Fox J:Helicobacter felis gastric infection in gnotobiotic beagle dogs. Infect Immun 58:2606–2612, 1990

    PubMed  Google Scholar 

  11. Cave DR, Vargas M: Effect of aCampylobacter pylori protein on acid secretion by parietal cells. Lancet 2:187–189, 1989

    PubMed  Google Scholar 

  12. Vargas M, Lee A, Fox JG, and Cave DR: Inhibition of acid secretion from parietal cells by non-human-infectingHelicobacter species: A factor in colonization of gastric mucosa? Infect Immun 59:3694–3699, 1991

    PubMed  Google Scholar 

  13. Defize J, Goldie J, Hunt RH: Inhibition of acid production byCampylobacter pylori in isolated guinea pig parietal cells. Gastroenterology 96:A114, 1989

    Google Scholar 

  14. Jalanowski H, Kramer N, Hengels KJ: Effect ofHelicobacter pylori on acid secretion by isolated human gastric glands. Gastroenterology 100:A90, 1991

    Google Scholar 

  15. Berglindh T, Obrink KJ: A method for preparing isolated glands from the rabbit gastric mucosa. Acta Physiol Scand 96:150–159, 1976

    PubMed  Google Scholar 

  16. Raufman JP, Sutliff VE, Kasbekar DK, Jensen RT, Gardner JD: Pepsinogen secretion from dispersed chief cells from guinea pig stomach. Am J Physiol 247:G95-G104, 1984

    PubMed  Google Scholar 

  17. Cabaud PG, Wroblewski F: Colorimetric measurement of lactic dehydrogenase activity of body fluids. Am J Clin Pathol 30:234–236, 1958

    PubMed  Google Scholar 

  18. Leunk RD, Johnson PT, David BC, Kraft WG, Morgan DR: Cytotoxic activity in broth-culture filtrates ofCampylobacter pylori. J Med Microbiol 26:93–99, 1988

    PubMed  Google Scholar 

  19. Smoot DT, Mobley HL, Chippendale GR, Lewison JF, Resau JH:Helicobacter pylori urease activity is toxic to human gastric glands cells. Infect Immun 58:1992–1994, 1990

    PubMed  Google Scholar 

  20. Cave DR, King WW, Hoffman JS: Production of two chemically distinct acid-inhibitory factors byHelicobacter pylori. Eur J Gastroenterol Hepatol 5:S23-S27, 1993

    Google Scholar 

  21. Chen XG, Correa P, Offerhaus J, Rodriguez E, Janney F, Hoffmann E, Fox J, Hunter F, Diavolitsis S: Ultrastructure of the gastric mucosa harboringCampylobacter-like organisms. Am J Clin Pathol 86:575–582, 1986

    PubMed  Google Scholar 

  22. Isenberg JE, McQuaid KR, Laine L, Rubin W: Acid-peptic disorders.In Textbook of Gastroenterology, Vol. 1. T. Yamada (ed). Philadelphia, J. B. Lippincott, 1991, pp 1241–1244

    Google Scholar 

  23. Miller JF, Mekalanos JJ, Falkow S: Coordinate regulation and sensory transduction in the control of bacterial virulence. Science 243:916–922, 1989

    PubMed  Google Scholar 

  24. Morris AJ, Ali MR, Nicholson GI, Perez-Perez GI, Blaser MJ: Long-term follow-up of voluntary ingestion ofHelicobacter pylori. Ann Intern Med 114:662–663, 1991

    PubMed  Google Scholar 

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Author notes

  1. David R. Cave MD

    Present address: Division of Gastroenterology, Department of Medicine, St. Elizabeth's Hospital of Boston, Tufts University School of Medicine, Boston, Massachusetts

Authors and Affiliations

  1. From the Section of Gastroenterology, Evans Memorial Department of Clinical Research, Boston University School of medicine, Boston, Massachusetts

    James S. Hoffman MD, William W. King MD, James G. Fox DVM, Dale Janik MD &  David R. Cave MD

  2. Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts

    James S. Hoffman MD, William W. King MD, James G. Fox DVM, Dale Janik MD &  David R. Cave MD

Authors
  1. James S. Hoffman MD
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  2. William W. King MD
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  3. James G. Fox DVM
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  4. Dale Janik MD
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  5. David R. Cave MD
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Hoffman, J.S., King, W.W., Fox, J.G. et al. Rabbit and ferret parietal cell inhibition byHelicobacter species. Digest Dis Sci 40, 147–152 (1995). https://doi.org/10.1007/BF02063958

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  • DOI: https://doi.org/10.1007/BF02063958

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