Skip to main content
SpringerLink
Log in

Occurrence of antibody against rat hepatic sinusoidal endothelial cells in sera of patients with autoimmune hepatitis

  • Liver: Infectious, Inflammatory, And Metabolic Disorders
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

To determine whether an antibody against hepatic sinusoidal endothelial cells was present in sera of patients with autoimmune hepatitis (AIH) type 1, we measured the serum IgG bound to the glutaraldehyde-fixed cultured rat sinusoidal endothelial cells by enzyme-linked immunosorbent assay. IgG bound to the cells was detected significantly more in patients with autoimmune hepatitis type 1 (97.1%) than in those with primary biliary cirrhosis (13.0%), chronic hepatitis C (5.9%) or B (7.9%), or healthy controls (0%). IgG-F(ab')2 fragments from autoimmune hepatitis patients also bound to the cells, and this binding was observed after absorption of the fragments with rat hepatoma cells, but not after absorption with bovine carotid endothelial cells. Culture of sinusoidal endothelial cells in the presence of IgG from AIH patients significantly reduced the number of viable attached cells. In conclusion, anti-sinusoidal endothelial cell antibody occurred in the sera from patients with autoimmune hepatitis type 1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Mackay IR, Weiden S, Hasker J: Autoimmune hepatitis. Ann NY Acad Sci 124:767–780, 1965

    PubMed  Google Scholar 

  2. Johnson PJ, McFarlane IG: Meeting report: International autoimmune hepatitis group. Hepatology 18:998–1005, 1993

    PubMed  Google Scholar 

  3. Manns M, Gerren G, Kyriatsoulis A, Staritz M, Meyer zum Buschenfelde KH: Characterization of a new subgroup of autoimmune chronic active hepatitis by autoantibodies against a soluble liver antigen. Lancet 1:292–294, 1987

    PubMed  Google Scholar 

  4. Toda G, Ikeda Y, Hashimoto N, Yamazaki M, Torii M, Oka H: Liver cell membrane antibody detected by protein A and isolated rabbit liver plasma membrane in sera of patients with chronic liver diseases. Clin Exp Immunol 54:661–670, 1983

    PubMed  Google Scholar 

  5. Toda G, Ikeda Y, Kashiwagi M, Iwamori M, Oka H: Hepatocyte plasma membrane glycosphingolipid reactive with sera from patients with autoimmune chronic active hepatitis: Its identification as sulfatide. Hepatology 12:664–670, 1990

    PubMed  Google Scholar 

  6. McFarlane IG: Autoimmunity in liver disease. Clin Sci 67:569–578, 1984

    PubMed  Google Scholar 

  7. Quadros NP, Roberto-Thomson PJ, Gallus AS: IgG and IgM anti-endothelial cell antibodies in patients with collagen-vascular disorders. Rheumatol Int 10:113–119, 1990

    PubMed  Google Scholar 

  8. Rosenbaum J, Pottinger BE, Woo P, Black CM, Loizou S, Byron MA, Pearson JD: Measurement and characterization of circulating anti-endothelial cell IgG in connective tissue diseases. Clin Exp Immunol 72:450–456, 1988

    PubMed  Google Scholar 

  9. Cines DB, Lyss AP, Reeber M, Bina M, DeHoratius RJ: Presence of complement-fixing anti-endothelial cell antibodies in systemic lupus erythematosus. J Clin Invest 73:611–625, 1984

    PubMed  Google Scholar 

  10. Savage COS, Pottinger BE, Gaskin G, Lockwood CM, Pusey CD, Pearson JD: Vascular damage in Wegener's granulomatosis and microscopic polyarteritis: presence of anti-endothelial cell antibodies and their relation to anti-neutrophil cytoplasm antibodies. Clin Exp Immunol 85:14–19, 1991

    PubMed  Google Scholar 

  11. Penning CA, Cunningham J, French MAH, Harrison G, Powell NR, Hughes P: Antibody-dependent cellular cytotoxicity of human vascular endothelium in systemic sclerosis. Clin Exp Immunol 57:548–556, 1984

    PubMed  Google Scholar 

  12. Leung DYM, Collins T, Lapierre LA, Geha RS, Pober JS: Immunoglobulin M antibodies present in the acute phase of Kawasaki syndrome lyse cultured vascular endothelial cells stimulated by gamma interferon. J Clin Invest 77:1428–1435, 1986

    PubMed  Google Scholar 

  13. Leung DYM, Moake JL, Havens PL, Kim M, Pober JS: Lytic anti-endothelial cell antibodies in haemolytic-uremic syndrome. Lancet 1:183–186, 1988

    Google Scholar 

  14. Holt CM, Lindsey N, Moult J, Malia RG, Greaves M, Hume A, Rowell NR, Hughes P: Antibody-dependent cellular cytotoxicity of vascular endothelium: Characterization and pathogenic associations in systemic sclerosis. Clin Exp Immunol 78:359–365, 1989

    PubMed  Google Scholar 

  15. Okamoto H, Okada S, Sugiyama Y, Tanaka T, Sugai Y, Akahane Y, Machida A, Mishiro S, Yoshizawa H, Miyakawa Y, Mayumi M: Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5′-noncoding region. Jpn J Exp Med 60:215–222, 1990

    PubMed  Google Scholar 

  16. Abuaf N, Lunel F, Giral P, Borotto E, Laperche S, Poupon R, Opolon P, Huraux JM, Homberg C: Non-organ specific autoantibodies associated with chronic C virus hepatitis. J Hepatol 18:359–364, 1993

    PubMed  Google Scholar 

  17. Scheuer PJ: Primary biliary cirrhosis. Proc R Soc Ned 60:1257–1260, 1967

    Google Scholar 

  18. Ludwig J, Members of the Working Party: Terminology of chronic hepatitis, hepatic allograft rejection, and nodular legions of the liver: Summary of recommendations developed by an International Working Party, supported by the World Congress of Gastroenterology, Los Angeles, 1994. Am J Gastroenterol 89:S177-S181, 1994

    PubMed  Google Scholar 

  19. Knook DL, Blansjaar N, Sleyster ECH: Isolation and characterization of Kupffer and endothelial cells from the rat liver. Exp Cell Res 109:317–329, 1977

    PubMed  Google Scholar 

  20. Hjelm H, Hjelm K, Sjoquist J: Protein A fromStaphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins. FEBS Lett 28:73–76, 1972

    PubMed  Google Scholar 

  21. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275, 1951

    PubMed  Google Scholar 

  22. Hashimoto N, Watanabe T, Ikeda Y, Toda G, Yamada H, Yoshikawa Y, Mitsui H, Kurokawa K: Down-regulation of prostaglandin E2 receptors in regenerating rat liver and its physiological significance. Biochem Biophys Res Commun 177:226–232, 1991

    Google Scholar 

  23. Emori T, Hirata Y, Ohta K, Sichiri M, Marumo F: Secretory mechanism of immunoreactive endothelin in cultured bovine endothelial cells. Biochem Biophys Rec Commun 160:93–100, 1989

    Google Scholar 

  24. Nolan JS, Packer L: Monolayer culture techniques for normal human diploid fibroblasts, Methods Enzymol 32:561–574, 1974

    PubMed  Google Scholar 

  25. Muro H, Shirasawa H, Maeda M, Nakamura S: Fc receptors of liver sinusoidal endothelium in normal rats and humans. Gastroenterology 93:1078–1085, 1987

    PubMed  Google Scholar 

  26. Vento S, Eddleston ALWF: Immunological aspects of chronic active hepatitis. Clin Exp Immunol 68:225–231, 1978

    Google Scholar 

  27. Meyer zum Buschenfelde K-H, Miescher PA: Liver specific antigens: Purification and characterization. Clin Exp Immunol 10:89–102, 1972

    PubMed  Google Scholar 

  28. Jensen DM, McFarlane IG, Portmann BS, Path MRC, Eddleston ALWF, Williams R: Detection of antibodies directed against a liver-specific membrane lipoprotein in patients with acute and chronic active hepatitis. N Engl J Med 299:1–7, 1987

    Google Scholar 

  29. Hopf U, Meyer zum Buschenfelde K-H, Arnold W: Detection of a liver membrane autoantibody in HBsAg-negative chronic active hepatitis. N Engl J Med 294:578–582, 1976

    PubMed  Google Scholar 

  30. Treichel U, Poralla T, Hess G, Manns M, Meyer zum Buschenfelde K-H: Autoantibodies to human asialoglycoprotein receptor in autoimmune chronic hepatitis. Hepatology 11:606–612, 1990

    PubMed  Google Scholar 

  31. Kosugi I, Muro H, Shirasawa H, Ito I: Endocytosis of soluble IgG immune complex and its transport to lysosomes in hepatic sinusoidal endothelial cells. J Hepatol 16:106–114, 1992

    PubMed  Google Scholar 

  32. Mishiro S, Hoshi Y, Takeda K, Yshikawa A, Gotanda T, Takahashi K, Akahane Y, Yashizawa H, Okamato H, Tsuda F, Peterson DA, Muchmore E: Non-A, non-B hepatitis specific antibodies directed at host-derived epitope: implication for an autoimmune process. Lancet 336:1400–1403, 1990

    PubMed  Google Scholar 

  33. Sipos J, McFarlane BM, Williams R, McFarlane IG: Sensitive avidin biotin based technique for identifying liver membrane antigens in tissue sections. J Clin Pathol 41:1217–1222, 1988

    PubMed  Google Scholar 

  34. Westphal JR, Boerbooms AMTH, Schalkwijk CJM, Kwast H, De Weijert M, Jacobs C, Vierwinden G, Ruiter DJ, Van de Putte LBA, De Waal RMW: Anti-endothelial cell antibodies in sera of patients with autoimmune diseases: Comparison between ELISA and FACS analysis. Clin Exp Immunol 96:444–449, 1994

    PubMed  Google Scholar 

  35. Wall RT, Harlan JM, Harker LA, Striker GE: Homocysteine-induced endothelial cell injuryin vitro: A model for the study of vascular injury. Thromb Res 18:113–121, 1980

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113, Tokyo, Japan

    Katsuken Han MD, Naoaki Hashimoto MD, PhD, Yusei Ikeda MD, PhD, Yasushi Shiratori MD, PhD, Hirokazu Kato MD, PhD, Gotaro Toda MD, PhD, Yutaka Komatsu MD, Haruki Yamada MD, Atsushi Tanaka MD & Kiyoshi Kurokawa MD, PhD

  2. Department of Internal Medicine, Tokyo Kosei-Nenkin Hospital, Shin'juku-ku, 162, Tokyo, Japan

    Katsuken Han MD, Naoaki Hashimoto MD, PhD, Yusei Ikeda MD, PhD, Yasushi Shiratori MD, PhD, Hirokazu Kato MD, PhD, Gotaro Toda MD, PhD, Yutaka Komatsu MD, Haruki Yamada MD, Atsushi Tanaka MD & Kiyoshi Kurokawa MD, PhD

  3. Department of Internal Medicine, Omiya Red Cross Hospital, Yono-si, 338, Saitama, Japan

    Katsuken Han MD, Naoaki Hashimoto MD, PhD, Yusei Ikeda MD, PhD, Yasushi Shiratori MD, PhD, Hirokazu Kato MD, PhD, Gotaro Toda MD, PhD, Yutaka Komatsu MD, Haruki Yamada MD, Atsushi Tanaka MD & Kiyoshi Kurokawa MD, PhD

  4. First Department of Internal Medicine, Jikei University School of Medicine, Minato-ku, 105, Tokyo, Japan

    Katsuken Han MD, Naoaki Hashimoto MD, PhD, Yusei Ikeda MD, PhD, Yasushi Shiratori MD, PhD, Hirokazu Kato MD, PhD, Gotaro Toda MD, PhD, Yutaka Komatsu MD, Haruki Yamada MD, Atsushi Tanaka MD & Kiyoshi Kurokawa MD, PhD

Authors
  1. Katsuken Han MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Naoaki Hashimoto MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yusei Ikeda MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yasushi Shiratori MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hirokazu Kato MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Gotaro Toda MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yutaka Komatsu MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Haruki Yamada MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Atsushi Tanaka MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Kiyoshi Kurokawa MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported in part by grants from the Ministry of Education, Science and Culture of Japan, Mitsui Life Social Welfare Foundation and Asahi Life Insurance Foundation.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Han, K., Hashimoto, N., Ikeda, Y. et al. Occurrence of antibody against rat hepatic sinusoidal endothelial cells in sera of patients with autoimmune hepatitis. Digest Dis Sci 40, 1213–1220 (1995). https://doi.org/10.1007/BF02065526

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02065526

Key words

Search

Navigation