Skip to main content
SpringerLink
Log in

Adenosine deaminase, 5′-nucleotidase, xanthine oxidase, superoxide dismutase, and catalase activities in gastric juices from patients with gastric cancer, ulcer, and atrophic gastritis

  • Original Articles
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

Adenosine deaminase (ADA), 5′-Nucleotidase (5NT), Xanthine oxidase (XO), Cu-Zn Superoxide dismutase (SOD) and Catalase (CAT) activities were determined in gastric juices from patients with gastric cancer, ulcer, gastritis and from healthy subjects. Enzyme activities were given as units per ml gastric juice and units per mg protein in gastric juice. ADA, 5NT and XO activities were found lower and protein concentrations were found higher in the cancer group than controls. There was however no significant difference between Cu-Zn SOD activities of the cancer and control groups. In all groups including control one, we could not find catalase activities in most of the samples. On the other hand, ADA, 5NT activities and protein concentrations in the gastric juice were lower in the gastritis group than control group. In the ulcer group, we found higher Cu-Zn SOD and XO activities and lower 5NT activity and protein concentrations compared with control values. In an attempt to establish statistical correlations between mean enzyme activities, pH and protein concentrations in the gastric juices of the groups, we found noticeable intra and inter-correlations, which indicated possible relations between DNA and free radical metabolizing enzymes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Vo TKO, Druez C, Delzenne N, Taper HS, Roberfroid M: Analysis of antioxidant defence systems during rat hepatocarcinogenesis. Carcinogenesis 9:2009–2013, 1988

    Google Scholar 

  2. Corrocher R, Carasil M, Bellisola G, Gabrielli GB, Nicoli N, Guidi GC, Sandre GD: Severe impairment of antioxidant systems in human hepatoma. Cancer 58:1658–1662, 1986

    Google Scholar 

  3. Goldstein BD, Czerniecki B, Witz G: The role of free radicals in tumor promotion. Environ Health Perspect 81:55–57, 1989

    Google Scholar 

  4. Hoffman CEJ, Webster NR, Wiggins PA, Chisholm EM, Giles GR, Leveson SH: Free radical detoxifying systems in human colorectal cancer. Br J Cancer 51:127–129, 1985

    Google Scholar 

  5. Van Balgooy JNA, Roberts E: Superoxide dismutase in normal and malignant tissues in different species. Comp Biochem Physiol 62B:263–268, 1979

    Google Scholar 

  6. Foulds L: Neoplastic Development, Vols 1 and 2. London, Academic Press, 1969/1975

    Google Scholar 

  7. Pitot HC: Biological and enzymatic events in chemical carcinogenesis. Annu Rev Med 30:25–39, 1979

    Google Scholar 

  8. Farber E, Cameron R: The sequential analysis of cancer development. Adv Cancer Res 35:125–126, 1980

    Google Scholar 

  9. Cerutti PA: Prooxidant states and tumor promotion. Science 227:375–381, 1985

    Google Scholar 

  10. Kozumbo WJ, Trush MA, Kensler TW: Are free radicals involved in tumor promotion? Chem Biol Interact 54:199–207, 1985

    Google Scholar 

  11. Player TJ, Mills PL, Horton AA: Age-dependent changes in rat liver microsomal and mitocondrial NADPH-dependent lipid peroxidation. Biochem Biophys Res Common 78:397–402, 1977

    Google Scholar 

  12. Freeman BA, Crapo JD: Biology of disease, free radicals and tissue injury. Lab Invest 47:412–426, 1982

    Google Scholar 

  13. Hecker E, Fusening N, Marks WT: Carcinogenesis: A comprehensive survey, Vol 7. New York, Raven Press, 1982

    Google Scholar 

  14. Kaplan JH, Groses JN: Liver and blood cell catalase activity of tumor bearing mice. Cancer Res 26:1190–1194, 1972

    Google Scholar 

  15. Ohuma T, Maldia G, Holland JF: Hepatic catalase activity in advanced human cancer. Cancer Res 26:1806–1818, 1966

    Google Scholar 

  16. Peskin AV, Koen YM, Zbazsky IB: Superoxide dismutase and glutathione peroxidase activities in tumors. FEBS Lett 1:41–45, 1978

    Google Scholar 

  17. Prajda N, Morris HP, Weber G: Imbalance of purine metabolism in hepatomas of different growth rates as expressed in behavior of xanthine oxidase (1.2.3.2.). Cancer Res 36:3639–3646, 1976

    Google Scholar 

  18. Weber G, Hager JC, Lui MS, Prajda N, Tzeng DY, Jackson RC, Takeda E, Eble JN: Biochemical programs of slowly and rapidly growing human colon carcinoma xenografts. Cancer Res 41:854–859, 1981

    Google Scholar 

  19. Koizumi H, Lizuka H, Aoyagi T, Miura Y: Adenosine deaminase in human epidermis from healthy and psoriatic subjects. Arch Dermatol Res 275:310–314, 1983

    Google Scholar 

  20. Koizumi H, Iizuka H, Aoyagi T, Miura Y: Characterization of adenosine deaminase from normal human epidermis and squamous cell carcinoma of the skin. J Invest Dermatol 84:199–202, 1985

    Google Scholar 

  21. Koizumi H, Iizuka H, Aoyagi T, Morioka M, Sakurada K: Adenosine deaminase activity in adult T cell leukemia. Proc Jpn Soc Invest Dermatol 5:51–52, 1980

    Google Scholar 

  22. Iizuka H, Koizumi H, Kimura T, Miura Y: Adenosine deaminase activity in peripheral lymphocytes of psoriasis and Sezary's syndrom. J Dermatol (Tokyo) 7:165–169, 1980

    Google Scholar 

  23. Lal H, Munjal SK, Wig U, Saini AS: Serum enzymes in head and neck cancer III. J Laryngol Otol 101:1062–1065, 1987

    Google Scholar 

  24. Camici M, Tozzi MG, Allegrini S, Corso AD, Sanfilippo O, Daidone MG, Marco CD, Ipata PL: Purine salvage enzyme activities in normal and neoplastic human tissues. Cancer Biochem Biophys 11:201–209, 1990

    Google Scholar 

  25. Kate JT, Wijnen JT, van der Goes RGM, Quadt R, Griffioen G, Bosman FT, Khan PM: Quantitative changes in adenosine deaminase isozymes in human colorectal adenocarcinomas. Cancer Res 44:4688–4692, 1984

    Google Scholar 

  26. Murray JL, Perez-Soler R, Bywaters D, Hersh EM: Decreased adenosine deaminase (ADA) and 5′ nucleotidase (5NT) activity in peripheral blood T cells in hodgkin disease. Am J Hematol 21:57–66, 1986

    Google Scholar 

  27. Meier J, Coleman MS, Hutton JJ: Adenosine deaminase activity in peripheral blood cells of patients with haematological malignancies. Br J Cancer 33:312–319, 1976

    Google Scholar 

  28. Tung R, Silber R, Quagliata F: Adenosine deaminase activity in chronic lymphocytic leukemia: Relationship to B and T cell subpopulations. J Clin Invest 57:756–761, 1976

    Google Scholar 

  29. Dasmahapatra KS, Hill HZ, Dasmahapatra A, Saure S: Evaluation of adenosine deaminase activity in patients with head and neck cancer. J Surg Res 40:368–373, 1986

    Google Scholar 

  30. Russo M, Giancane R, Apice G, Galanti B: Adenosine deaminase and purine nucleoside phosphorylase activities in peripheral lymphocytes from patients with solid tumors. Br J Cancer 43:196–200, 1981

    Google Scholar 

  31. Kojima O, Majima T, Uehara Y, Yamane T, Fujita Y, Takahashi T, Majima S: Alteration of adenosine deaminase levels in peripheral blood lymphocytes of patients with gastric cancer. Jpn J Surg 15:130–133, 1985

    Google Scholar 

  32. Raz A, Collard JG, Inbar M: Decrease in 5′ nucleotidase activity in malignant transformed and normal stimulated cells. Cancer Res 38:1258–1262, 1978

    Google Scholar 

  33. Lopez J, Zucker-Franklin D, Silber R: Heterogeneity of 5′ nucleotidase activity in lymphocytes in chronic lymphocytic leukemia. J Clin Invest 52:1297–1300, 1973

    Google Scholar 

  34. Quagliata F, Faig D, Conklyn M, Gorski J: Studies on the lymphocyte 5′ nucleotidase in chronic lymphocytic leukemia, infectious mononucleosis, normal populations and phytohemagglutinin-stimulated cells. Cancer Res 34:3197–3202, 1974

    Google Scholar 

  35. Namiot Z, Rutkiewicz J, Stasiewicz J, Gorski J: Adenosine deaminase activity in the human gastric mucosa in relation to acid secretion. Digestion 45:172–175, 1990

    Google Scholar 

  36. Minami M, Yoshikava M: A simplified assay method of superoxide dismutase activity for clinical use. Clin Chim Acta 92:337–342, 1979

    Google Scholar 

  37. Lowry O, Rosebrough N, Farr L, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1951

    Google Scholar 

  38. Giusti G: Adenosine deaminase.In Methods of Enzymatic Analysis. VH Bergmeyer and Weinheim (eds). Deerfield Beach, Florida, Verlag chemie, 1974, p 1092

    Google Scholar 

  39. Donald WM: EnzymesIn Textbook of Clinical Chemistry. NW Tietz (ed). Philadelphia, Saunders, 1986, pp 718–720

    Google Scholar 

  40. Sun Y, Oberley LW, Li Y: A simple method for clinical assay of superoxide dismutase. Clin Chim 34(3):497–500, 1988

    Google Scholar 

  41. Prajda N, Weber G: Malignant transformation linked imbalance decreased XO activity in hepatomas. FEBS Lett 59:245–249, 1975

    Google Scholar 

  42. Aebi H: CatalaseIn Methods of Enzymatic Analysis, Vols 1 and 2. HU Bergmeyer (ed). New York, Academic, 1974, pp 673–677

    Google Scholar 

  43. Donofrio J, Coleman MS, Hutton JJ, Daoud A, Lampkin B, Dyminski J: Overproduction of adenine deoxynucleosides and deoxynucleotides in adenosine deaminase deficiency with severe combined immunodeficiency disease. J Clin Invest 62:884–887, 1978

    Google Scholar 

  44. Hersfield MS, Kredich NM: Resistance of adenosine kinase-defficient human lymphoblastoid cell line to effects of deoxyadenosine on growth.S-adenosylhomocysteine hydrolase inactivation, and DNA accumulation. Proc Natl Acat Sci USA 771:4292–4296, 1980

    Google Scholar 

  45. Gerber JG, Faulds,, Payne NA, Niej AS: Adenosine: A modulator of gastric acid secretionin vivo. J Pharmacol Exp Ther 231:109–113, 1984

    Google Scholar 

  46. Gerber JG, Nies AS, Payne NA: Adenosine reseptors on canine parietal cells modulate gastric acid secretion to histamine. J Pharmacol Exp Ther 233:623–627, 1985

    Google Scholar 

  47. Ushijima I, Mizuki Y, Yamada M: Development of stress-induced gastric lesions involves central adenosine A1 receptor stimulation. Brain Res 339:351–355, 1985

    Google Scholar 

  48. Westerberg VS, Glavin GB, Geiger JD: Intracerebroventricular administration of (-)-phenylisopropyladenosine protects rats against stress-induced ulcer formation. Proc West Pharmacol Soc 29:425–427, 1986

    Google Scholar 

  49. Gerber JG, Payne NA: Endogenous adenosine modulates gastric acid secretion to histamine in canine parietal cells. J Pharmacol Exp Ther 244:190–194, 1988

    Google Scholar 

  50. Baron MD, Pope B, Luzio JP: The membrane topography of ecto-5′-nucleotidase in rat hepatocytes. Biochem J 236:495–502, 1986

    Google Scholar 

  51. Meisel AD, Natarajan C, Sterba G, Diamond HS: Cyclic nucleotide function by adenosine deaminase inhibition. Adv Exp Med Biol 122:251–257, 1979

    Google Scholar 

  52. Wolberg G, Zimmerman TP, Hienstra K: Adenosine inhibition of lymphocyte-mediated cytolysis. Possible role of cyclic adenosine monophosphate. Science 187:957–959, 1975

    Google Scholar 

  53. Wilson JM, Mitchell BS, Daddona PE, Kelley WN: Differential effects of deoxyadenosine and deoxyguanosine on DNA synthesis in human T-lymphoblasts. J Clin Invest 64:1475–1484, 1979

    Google Scholar 

  54. Herschfield MS: Apparent suicide inactivation of human lymphoblastS-adenosylhomocystein hydrolase by 2-deoxyadenosine and adenine arabinoside: A basis for direct toxic effects of analogs of adenosine. J Biol Chem 254:22–25, 1979

    Google Scholar 

  55. Birnboim HC: DNA strand breakage in human leukocytes exposed to a tumor promoter, phorbol myristate acetate. Science 215:1247–1249, 1982

    Google Scholar 

  56. Schwarz M, Peres G, Kunz W, Furstenberger G, Kittstein W, Marks F: On the role of superoxide anion radicals in skin tumor promotion. Carcinogenesis 5:1663–1670, 1984

    Google Scholar 

  57. Nakamura Y, Colburn NH, Gindhart TD: Role of reactive oxygene in tumor promotion: Implication of superoxide anion in promotion of neoplastic transformation in JB-6 cells by TPA. Carcinogenesis 6:229–235, 1985

    Google Scholar 

  58. Yam J, Frank L, Roberts RJ: Oxygen toxicity: Comparison of lung biochemical responses in neonatal and adult rats. Pediatr Res 12:115–119, 1978

    Google Scholar 

  59. Singh A, Singh H: Time scale and nature or radiation biochemical damage: Approached to therapy. Prog Biophys Mol Biol 39:69–107, 1982

    Google Scholar 

  60. Myers CE, McGuire WP, Lisa RH: Adriamycin: The role of lipid peroxidation in cardiac toxicity and tumor response. Science 197:165–167, 1977

    Google Scholar 

  61. Brozdz M, Luciak M, Jendryczko A, Magner K: Changes in lung activity of superoxide dismutase and copper concentration during lung tumorigenesis by hydralasine in Swiss mice. Exp Pathol 32:119–122, 1987

    Google Scholar 

  62. Perchellet EM, Abney NL, Perchellet J-P: Stimulation of hydroperoxide generation in mouse skins treated with tumor-promoting or carcinogenetic agentsin vivo andin vitro. Cancer Lett 42:169–177, 1988

    Google Scholar 

  63. Beauchamp C, Fridovich I: Superoxide dismutase: improved assays and an applicable to acrylamide gels. Anal Biochem 44:276–287, 1971

    Google Scholar 

  64. Paul LA, Fulton AM, Heppner GH: Reactive oxygene-mediated damage to murine mammary tumor cells. Mutat Res 215:223–234, 1989

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, From The Ankara University, Faculty of Medicine, Ankara, Turkey

    Ilker Durak PhD, Necati Örmeci MD, Ömer Akyol MD, Orhan Canbolat MD, Mustafa Kavutçu BA & Mahmut Bülbül MD

Authors
  1. Ilker Durak PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Necati Örmeci MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ömer Akyol MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Orhan Canbolat MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mustafa Kavutçu BA
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mahmut Bülbül MD
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Durak, I., Örmeci, N., Akyol, Ö. et al. Adenosine deaminase, 5′-nucleotidase, xanthine oxidase, superoxide dismutase, and catalase activities in gastric juices from patients with gastric cancer, ulcer, and atrophic gastritis. Digest Dis Sci 39, 721–728 (1994). https://doi.org/10.1007/BF02087413

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02087413

Key words

Search

Navigation