Abstract
Granuloma formation was studied in rats following s.c. implantation of polyether sponges soaked in carrageenin. Sponges, which had been boiled in ethanol, produced larger granulomata and more exudate than unboiled sponges, but prostaglandin (PG) concentrations were lower in exudates from boiled sponges.
Sub-cutaneous injection of the ethanolic sponge extract inhibited the delayed phase of carrageenin-induced rat hind paw oedema. A counter irritant action was ruled out by the demonstration that local administration of the extract also inhibited hind paw oedema.
Ultra-violet spectrophotometry of the anti-inflammatory extract indicated that the main constituent was probably a phenolic material, commonly used as an antioxidant in the manufacture of polymers. The antioxidant nature of the extract was confirmed by its inhibition of the autoxidation of adrenaline.
The results are discussed in the light of recent reports on the anti-inflammatory properties of phenolic antioxidants and their effects on the PG biosynthetic pathway.
Similar content being viewed by others
References
H. Selye,The Diseases of Adaptation: Introductory Remarks, Rec. Progr. Horm. Res.8, 117–142 (1953).
P.N. Saxena,Effect of Drugs on Early Inflammatory Reaction, Arch. int. pharmacodyn.126, 228–237 (1960).
E. Kulonen andM. Potila,Effect of the Administration of Antirheumatic Drugs on Experimental Granuloma in Rat, Biochem. Pharmac.24, 219–225 (1975).
G.A. Higgs, E.A. Harvey, S.H. Ferreira andJ.R. Vane,The Effects of Antiinflammatory Drugs on the Production of Prostaglandins in vivo, inAdvances in Prostaglandin and Thromboxane Research, vol. 1 (Eds, B., Samuelsson and R. Paoletti; Raven Press, New York, 1976), p. 105.
I.L. Bonta, M.J. Parnham andM.J.P. Adolfs,Reduced Exudation and Increased Tissue Proliferation During Chronic Inflammation in Rats Deprived of Endogenous Prostaglandin Precursors, Prostaglandins14, 295–307 (1977).
M.J. Parnham, M.J.P. Adolfs andI.L. Bonta,The Effect of Metyrapone on Granuloma Induced by Carrageenin-Impregnated Sponges in Normal and Essential Fatty Acid Deficient Rats, J. Pharm. Pharmac. (in press).
A.W. Ford-Hutchinson, M.J.H. Smith, P.N.C. Elliott, J.P. Bolam, J.R. Walker, A.A. Lobo, J.K. Badcock, A.J. Colledge andF.J. Billimoria,Effects of a Human Plasma Fraction on Leucocyte Migration into Inflammatory Exudates, J. Pharm. Pharmac.27, 106–112 (1975).
H.P. Misra andI. Fridovich,The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase, J. Biol. Chem.247, 3170–3175 (1972).
I.L. Bonta,Endogenous Modulators of Inflammation, Agents and Actions, Suppl. 3, 121 (1977).
S. Goldstein, R. Demeo, I. Shemano andJ.M. Beiler,A Method for Differentiating Non-Specific Irritants from Anti-Inflammatory Agents, Using the Carrageenin Abscess Test, Proc. Soc. Exp. Biol. Med.123, 712–715 (1966).
R.W. Shanahan,Local Activity of Anti-Inflammatory and Irritant Agents on Rat Paw Oedema Induced by Carrageenin, Arch. int. Pharmacodyn.175, 186–192 (1968).
L. Levy andT.L. Kerley,The use of DPPD (N,N′-Diphenyl-p-phenylenediamine) as an Anti-Inflammatory Agent, Life Sci.14, 1917–1925 (1974).
H. Sobański, J. Krupińska, B. Cebo, J. Mazur andA. Kieć-dembinska,Antioxidants as Agents Potentiating the Anti-Inflammatory Action of Indomethacin, Acta Biol. Med. Germ.35, 1547–1552 (1976).
F.A. Kuehl, J.L. Humes, R.W. Egan, E.A. Ham, G.C. Beveridge andC.G. Van Arman,Role of Prostaglandin Endoperoxide PGG 2 in Inflammatory Processes, Nature265, 170–173 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Parnham, M.J., Bonta, I.L., Adolfs, M.J.P. et al. A polyether sponge constituent which is antioxidant, anti-inflammatory and increases prostaglandin concentrations at the inflamed site. Agents and Actions 7, 539–544 (1977). https://doi.org/10.1007/BF02111127
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02111127