Abstract
Guinea pigs were inoculated by intranasal inoculation with unadapted, influenza virus A/England/42/72, and virus was recovered from nasal washings between 3 and 10 days post-inoculation. Infected animals did not exhibit a febrile response to infection, did not produce local antibody and produced only relatively low levels of serum antibody. However, they developed delayed-type hypersensitivity to influenza virus, demonstrable by both skin tests and macrophage migration inhibition tests, which was similar to that of man. The relevance of the influenza virus specific delayed hypersensitivity in immunity to infection was examined in this model. Guinea pigs previously infected with virus or passively immunized with hyperimmune serum were relatively resistant to reinfection with influenza virus A/England/42/72. Inoculation of guinea pigs with spleen cells from immune donor animals, together with or without immune serum, did not give or enhance resistance to challenge virus infection. The results do not suggest a role for delayed hypersensitivity response in immunity to influenza virus infection.
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References
Beveridge, W.I.B., Burnet, F.M.: A cutaneous reaction in influenza viruses. Med. J. Australia1, 85–89 (1944)
Couch, R.B., Douglas, R.G., Rossen, R., Kasel, J.A.: Role of secretory antibody in influenza. In “The Secretory Immune System” pp. 93–113 U.S. Department of Public Health (1969)
David, J.R., Al-Askari, S., Lawrence, S., Thomas, L.: Delayed hypersensitivity in vitro. I. The Specificity of inhibition of cell migration by antigens. J. Immunol.93, 264–273 (1964)
Dolin, R., Richman, D.D., Murphy, B.R., Cauci, A.S.: Cell-mediated immune responses following influenza in man. Clin. Res.24, 342A (1976)
Fazekas de St. Groth, S., Witchell, S.J., Lafferty, K.J.: An improved assay for neutralization antibodies against influenza viruses. J. Hyg. (Camb.).56, 415–426 (1958)
Freestone, D.S., Hamilton-Smith, S., Schild, G.C., Buckland, R., Chinn, S., Tyrrell, D.A.J.: Antibody responses and resistance to challenge in volunteers vaccinated with live, attenuated, detergent split and oil adjuvant A2/Hong Kong/68 (H3N2) influenza vaccines. J. Hyg. (Camb.).70, 531–543 (1972)
Gitnick, G.L., Vincent, M.M., Auletta, A., Ley, A., Tzan, N., Sever, J.L.: Production of rubella haemagglutinating antigen in suspension cultures. Appl. Microbiol.15, 1244–1247 (1967)
Habershon, R.B., Molyneux, M.E., Slavin, G., Lowei, G., Tyrrell, D.A.J.: Skin tests with influenza virus. J. Hyg. (Camb.).71, 755–759 (1973)
Hobson, D., Beare, A.S., Ward-Gardner, A.: Haemagglutinin-inhibiting serum antibody titres as an index of response of volunteers to intranasal infection with a live, attenuated strain of influenza virus. Proceedings of the Symposium of Live Influenza Vaccines, Zagreb (1971) pp. 73–84 (Yugoslav Academy of Science and Arts, 1972)
Jennings, R., Denton, M.D., Potter, C.W.: The hamster as an experimental animal in the study of influenza. I. The role of antibody in protection. Med. Microbiol. Immunol.162, 217–226 (1976)
Jennings, R., Phair, J.P., Denton, M.D., Potter, C.W.: The hamster as an experimental animal for the study of influenza. II. The role of spleen cells in protection. Med. Microbiol. Immunol.162, 227–237 (1976)
Jurgensen, P.F., Olsen, G.N., Johnson, J.E., Swenson, E.W., Ayoub, E.M., Henney, C.S., Waldman, R.H.: Immune responses of the human respiratory tract. II. Cell-mediated immunity in the lower respiratory tract to tuberculosis and mumps and influenza viruses. J. Infect. Dis.28, 731–735 (1973)
McLaren, C., Potter, C.W.: Immunity to influenza in ferrets. VII. Effect of previous infection with heterotypic and heterologous influenza viruses on the response of ferrets to inactivated influenza virus vaccines. J. Hyg. (Camb.).72, 91–100 (1974)
Meiklejohn, G., Kempe, C.H., Tahalman, W.G., Lennette, E.H.: Evaluation of monovalent influenza vaccines. II. Observations during an influenza A-prime epidemic. Amer. J. Hyg.55, 12–21 (1952)
Potter, C.W., Oxford, J.S., Shore, S.L., McLaren, C., Stuart-Harris, C.H.: Immunity to Influenza in ferrets. I. Response to live and killed virus. Brit. J. Exp. Path.53, 153–167 (1972)
Potter, C.W., Rees, R.C., Shore, S.L., Jennings, R., McLaren, C.: Immunity to influenza in ferrets. IX. Delayed hypersensitivity following infection or immunization with A2/Hong Kong virus. Microbios.10, 7–21 (1974)
Reed, L.J., Muench, H.: A simple method for estimating 50 per cent endpoints. Amer. J. Hyg.27, 493–497 (1938)
Russell, S.M.D., McCahon, D., Beare, A.S.: A single radial haemolysis technique for the measurement of influenza antibodies. J. Gen. Virol.27, 1–10 (1975)
Schulman, J.L., Kilbourne, E.D.: Induction of partial specific immunity in mice by a single infection with influenza A virus. J. Bact.89, 170–174 (1965)
Small, P.A., Waldman, R.H., Bruno, J.C., Gifford, G.E.: Influenza infection in ferrets: role of serum antibody in protection and recovery. Inf. Imm.13, 417–424 (1976)
Virelizier, J.L.: Host defences against influenza virus: the role of anti-haemagglutinin antibody. J. Immunol.115, 434–439 (1976)
Waldman, R.H., Mann, J.J., Small, P.A. Jr.: Immunization against influenza: prevention of illness in man by aerosolized inactivated vaccine. J.A.M.A.207, 520–524 (1969)
Werner, G.H.: Immunité croisse'e hétérotypique chez la souris entre virus grippaux humains de types A et A2 C.R. Acad. Sci.263, 1913–1916 (1966)
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Phair, J.P., Kauffman, C.A., Jennings, R. et al. Influenza virus infection of the guinea pig: Immune response and resistance. Med Microbiol Immunol 165, 241–254 (1979). https://doi.org/10.1007/BF02152923
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DOI: https://doi.org/10.1007/BF02152923