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Portal hemodynamic changes after hepatocyte transplantation in acute hepatic failure

  • Original Paper
  • Published:
Journal of Biomedical Science

Abstract

Hepatocyte transplantation has been proposed as an alternative for rescuing patients with acute hepatic failure. However, portal hemodynamic changes and issues of safety after hepatocyte transplantation in acute hepatic failure have not been systemically evaluated because of the lack of a suitable experimentation system. In this study, we created a novel spring-guidewire introducer needle to simplify the technique for long-term portal cannulation in F-344 rats. The portal cannula was capable of being used for blood sampling, infusion of hepatocytes, and measurement of portal hemodynamic changes. One week after portal cannulation, rats were injected withD-galactosamine (1.35 g/kg, i.p.) to induce hepatic failure. Hepatocytes (2×107) were infused intraportally 24–26 h after induction of liver injury. Portal pressures were recorded for up to 60 min after hepatocyte transplantation. Intraportal infusion of 2×107 hepatocytes caused an instantaneous onset of portal hypertension. The magnitude of the rise in portal pressure was similar in both normal rats and rats with acute hepatic failure (33.0±7.1 vs. 37.7±0.5 mm Hg; p=0.23). However, the resolution rate of portal hypertension was remarkably delayed in rats with acute hepatic failure, and the portal pressure was significantly higher than that in normal rats 60 min after hepatocyte transplantation (25.0±2.8 vs. 14.5±2.4 mm Hg; p=0.007). In conclusion, we have established a simple new technique for long-term portal cannulation of rats. Our studies provide critical insights into the delayed resolution of portal hemodynamics after hepatocyte transplantation in subjects with acute hepatic failure.

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Authors and Affiliations

  1. Graduate Institute of Clinical Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, China

    Chun-Hsien Yu, Wei-Tsung Chen & Mei-Hwei Chang MD

  2. Hepatitis Research Center, National Taiwan University Hospital and National Taiwan University College of Medicine, China

    Hui-Ling Chen

  3. Laboratory Animal Center, National Taiwan University Hospital and National Taiwan University College of Medicine, China

    Yah-Luen Lin

  4. Department of Pediatrics, Taipei Municipal Women's and Children's Hospital, China

    Chun-Hsien Yu

  5. Department of Radiology, Taipei Municipal Jen-Ai Hospital, Taipei, Taiwan

    Wei-Tsung Chen

  6. Department of Pediatrics, National Taiwan University Hospital, 7 Chung-Shan S. Road, 100, Taipei, Taiwan

    Yen-Hsuan Ni & Mei-Hwei Chang MD

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  1. Chun-Hsien Yu
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  2. Hui-Ling Chen
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  3. Wei-Tsung Chen
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  5. Yah-Luen Lin
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  6. Mei-Hwei Chang MD
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Yu, CH., Chen, HL., Chen, WT. et al. Portal hemodynamic changes after hepatocyte transplantation in acute hepatic failure. J Biomed Sci 11, 756–763 (2004). https://doi.org/10.1007/BF02254360

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  • DOI: https://doi.org/10.1007/BF02254360

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